[Histonet] cryostat adapter

2009-01-30 Thread Leslie Chen
Hello, I have been trying to find a cryostat adapter for 6 months with no luck. Does anyone know where I can find an adapter to hold plastic embedding rings such as this: http://www.emsdiasum.com/microscopy/products/histology/imaages2/62350__.jpg We have a Leica and I've called Leica, called Therm

[Histonet] Fw: Derm processing times

2009-01-30 Thread jennifer cresor mike hough
- Original Message - From: jennifer cresor mike hough To: histonet@lists.utsouthwestern.edu Sent: Thursday, January 29, 2009 2:21 PM Subject: Derm processing times Hello all, I am looking for some feed back on tissue processing times that have worked well. The processor would be str

RE: [Histonet] Who can gross??

2009-01-30 Thread JoeNocito
I'm not short, just vertically challenged JTT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, January 30, 2009 12:52 PM To: Ford Royer; histonet Subject: RE

RE: [Histonet] JB4 users

2009-01-30 Thread Pamela Marcum
I agree with Gayle. GMA does not work well with mouse femurs. Not only are the issues with infiltration a problem, even when using mixtures of ethanol to pure GMA it still will not penetrate well and polymerizes for to quickly and too hot to make a good block. I personally find it too soft for

RE: [Histonet] Who can gross??

2009-01-30 Thread Bartlett, Jeanine (CDC/CCID/NCZVED)
Hey, I am one of those "short people" and I do NOT have tiny little teeth OR nasty little feet. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...

RE: [Histonet] Who can gross??

2009-01-30 Thread Ford Royer
I hear you Judy... you have to pick them up just to say hello! ;-) (Now before there is a flame of political correctness coming back at us... everyone lighten up. I AM one of those short people, and I can make fun of myself if I want too). ;-) Ford M. Royer, MT(ASCP) ...who use to have to sit

Re: [Histonet] Who can gross??

2009-01-30 Thread Peter Carroll
> Those are exactly the sources discussed in the archives. B-but... why check the archives when we can just ask the entire group the same questions repeatedly? ;-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthweste

RE: [Histonet] Who can gross??

2009-01-30 Thread Charles.Embrey
Those are exactly the sources discussed in the archives. -Original Message- From: Norm Burnham [mailto:norm.burn...@propath.com] Sent: Friday, January 30, 2009 12:09 PM To: Charles.Embrey; sheila adey Cc: histonet Subject: RE: [Histonet] Who can gross?? Better sources include: CAP Anatom

RE: [Histonet] Who can gross??

2009-01-30 Thread Norm Burnham
Better sources include: CAP Anatomic Pathology checklist or CLIA regulations -- which are quite specific as to "who can gross". Cheers, Norm Burnham ProPath www.propath.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.

RE: [Histonet] Who can gross??

2009-01-30 Thread Judith L. Williams
Now that folks is a beautiful Friday statement and comeback - those darn short techs really cause problems don't they :/ hehehehe Judy On Fri, 30 Jan 2009, Charles.Embrey wrote: As short histo techs what kind of aids are you using, stepladders or stools? Sorry, I couldn't resist. There is a

[Histonet] Presenters for HT/HTL Review Session for GSH

2009-01-30 Thread Shirley Powell
The names of the presenters for the HT/HTL review session was not on the program I first sent out on histonet. The review will be presented by Carl Sagasser, BS,HT(ASCP) Educational Coordinator and Taiquanda Winbush, AS, HT(ASCP) Clinical Laboratory Coordinator with the Darton College Histology On

RE: [Histonet] Who can gross??

2009-01-30 Thread Charles.Embrey
As short histo techs what kind of aids are you using, stepladders or stools? Sorry, I couldn't resist. There is a ton of information on the histonet archive about this. A search should yield all the info you seek and more. Charles Embrey, PA(ASCP) -Original Message- From: histonet-boun

RE: [Histonet] quiq decalcifier

2009-01-30 Thread gayle callis
Ben, Please clarify what you mean here and what species you are working with. Are you working with mouse heads with skull and whole brain intact? Or are there residual skull fragments or the does the brain contain some calcified areas (latter is something I personally have not experienced or hea

RE: [Histonet] JB4 users

2009-01-30 Thread gayle callis
GMA in general, does not infiltrate into calcified mouse femurs as well as methyl methacrylate, and controlling polymerization is not easy with calcified bone in GMA. You have already tried extended infiltrations, but didn't change the results. I suggest you change to a Technovits 9100 kit, and do

[Histonet] Who can gross??

2009-01-30 Thread sheila adey
Hi All, We are short histo techs and are using aids to help more and more. Our manager believes they can be doing the grossing of a histo tech as long as they have a tech or Dr to consult with. Can someone remind me of the guide lines for grossing. One has a BSc, is that enough? Thanks in a

Re: [Histonet] quiq decalcifier

2009-01-30 Thread Peter Carroll
> Embedded BRAIN tissue decalcifier? You know, I must have forgotten that today is friday, because I really passed up the opportunity to crack some fairly good puns at Ben's inquiry ;) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

RE: [Histonet] quiq decalcifier

2009-01-30 Thread Ford Royer
Fairly common procedure Rene with the percentage of boneheads in the population. I’ve got kids who use to live in my house who were boneheads. ;-) ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax

Re: [Histonet] consults with slide preparation

2009-01-30 Thread Rene J Buesa
He who signed the final diagnosis on this consultation ought to keep the slides because they are the "proof" of such final diagnosis. If you just did the IHC procedures to be sent to them so they would do the final diagnosis, then they keep the slides. René J. --- On Thu, 1/29/09, Houston, Rona

Re: [Histonet] quiq decalcifier

2009-01-30 Thread Rene J Buesa
Embedded BRAIN tissue decalcifier? Never heard this before! Leave and learn! René J. --- On Thu, 1/29/09, Ben Spirto wrote: From: Ben Spirto Subject: [Histonet] quiq decalcifier To: Histonet@lists.utsouthwestern.edu Date: Thursday, January 29, 2009, 12:20 PM Could someone tell me the best way

[Histonet] immunoportal?

2009-01-30 Thread Gudrun Lang
Hi all, has anyone information about the immunoportal website? It always shows errors, when I try to get in. www.immunoportal.com Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouth

Re: [Histonet] Safranin O stain in plastic sections

2009-01-30 Thread Peter Carroll
Gotta second this. I've done a lot of PMMA and/or Spurrs-embedded sectioning with it with good results. Jack Ratliff wrote: Yes, it works quite well for both demineralized and undemineralized bone sections embedded in resin. I perform MMA embedding (not a kit) and get really nice undemineraliz

Re: [Histonet] Safranin O stain in plastic sections

2009-01-30 Thread Jack Ratliff
Yes, it works quite well for both demineralized and undemineralized bone sections embedded in resin. I perform MMA embedding (not a kit) and get really nice undemineralized results. The resin I use allows me the opportunity to deplastify the sections just as one would deparaffinize when wor

[Histonet] just some ideas

2009-01-30 Thread Terri Braud
Message: 9 Date: Thu, 29 Jan 2009 13:19:25 -0700 From: "Sorenson, Jon (Nampa)" Subject: [Histonet] Document Control Thanks, Jon Jon Sorenson Histology Coordinator Mercy Medical Center jonsoren...@chiwest.com 208-463-5267 Does anyone in this group have any good ideas, either software or proce

[Histonet] Safranin O stain in plastic sections

2009-01-30 Thread Markus F. Meyenhofer
Hello all. Does Safranin O staining work on plastic sections (bone, decalcified)? Any help would be appreciated. Regards, Markus ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] JB4 users

2009-01-30 Thread Denise Crowley
We are a research core Histology facility accepting specimens from within the MIT community. One of our researchers is trying to embed mouse femurs in JB4 for us to section on our Thermo Finesse microtome utilizing a tungsten carbide blade.The tissue just crumbles out of the resin and

RE: [Histonet] Document Control

2009-01-30 Thread Vacca Jessica
If you have Outlook-tasks, you can set up reminders and even attach the p&p there as well, for you to review. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Thursday, January 29, 2009 4: