Hello!
Can anyone help us with CD markers? We want to use some CD markers on mouse
testis, and the antibodies data sheets explain that only freeze samples,
cryosectioned and post-fixed with acetone can be used. We tried to do this
protocols, but without any result. We frozen the sample
What markers and specific clones do you need help with specifically? Also mouse
testis really needs a PFA fix. Even if you do fresh frozen sections I suggest a
short (10 min) fix in PFA rather than acetone.
-Original Message-
From: Marti Gaudes, Merce mma...@cmrb.eu
Date: Wed, 04 Mar
Does anyone know a good anti-cholin acetyltransferase (ChAT) antibody for
mouse spinal cord to do immunohistochemistry (frozen section)? I see a
couple of vendors with more than a couple of clon names.
Moreover, somebody recommended this neurotransmitter as a motor neuronal
marker. If you can
Try some freshly prepared cresyl violet solutions?
2009-03-04
TF
发件人： Walters, Katherine S
发送时间： 2009-03-04 04:09:07
收件人： histo...@pathology.swmed.edu
抄送：
主题： [Histonet] Cresyl violet problems
To the brainy histo-people,
I have a student doing a cresyl violet stain on some frozen
Hello histonetters,
I have the following, in perfect working condition, items for sale:
tissue processor VIP1000 benchtop, just refurbished
Shandon 24-3 slide stainer
Surgipath PC3001, PC3002 embedding center
ducktless hood with new carbon filters
hood the a super quite vent
Hi,
I have some mouse hindlimbs that have catheterized with Omnipaque. The
Omnipaque contains 302 ml of iohexol which is equivalent to 140 mg of
organic iodine per ml. The mice will be sacrificed immediately after
being radiographed. They would like them to be processed in paraffin. I
am
Good Morning Victoria
I believe Market Lab carries plastic cassette holders.
Karen J Kay, MLT
Pathology Supervisor
Chinook Health Laboratory
Chinook Regional Hospital
960 - 19 st South
Lethbridge, Alberta, Canada
T1J 1W5
(403) 388 - 6061 (Phone)
(403) 388 - 6067 (Fax)
Message: 6
Date: Tue,
If there are not 12 to be found, document what you did, how you did it, and how
many you found.
You cannot find what is not there, but make sure you tried your best.
René J.
--- On Wed, 3/4/09, Smith Wanda wanda.sm...@hcahealthcare.com wrote:
From: Smith Wanda wanda.sm...@hcahealthcare.com
Hello histonetters,
Would you teach me what is best stain for elastic fiber in rat lung except
weigert's stain?
I want to look at arteries.
Thanks in advance,
Weihua
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hello everyone,
i have a problem which i dont know how to tackle, the
problem is that i have purchased vector blue as a chromogen and it is xylene
soluble can anyone suggest me how to avoid this as funds are low i cant
purchase xylene substitutes.
second question
Please help! We have been trying to cut whole eyes from a pig. They need to
be nice enough for a publication. We are having the devil of a time because no
matter what we do there are wrinkles. If we turn the waterbath up to stretch
things out the retina detaches. Do any of you have
Really? Liquid laundry soap? Does this help with all wrinkles in large
specimens?
Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397
--- On Wed,
Yes, really with any type of large (and small) sections. The liquid soap will
reduce the surface tension of water (more than heat) allowing the sections to
expand easily. Try it..
René J.
--- On Wed, 3/4/09, Va Paula Sicurello vapat...@yahoo.com wrote:
From: Va Paula Sicurello
My suggestion would be to follow the procedure of the old ocular guru
from the AFIP, Peter Emanuale. He taught me how the section human and
rabbit eyes. It involves using 2 water baths, one of which is at room
temp... and you should not use charged/coated slides.
Check our his chapter on ocular
If I am not mistaken vectamount is an aqueous mountant, dpx is
xylene/toluene based and requires going thru alcohols and xylene to mount.
One thing I do and you can try it, is airdry the slides and the use
permanent mounting media without going thru alcohols and xylene, keep in
mind there is a
Hi Vanessa,
I have read in at least one online protocol and have also been advised by
our excellent and experienced (former) histotech who used to be here at UB
NOT to warm the slides without air-drying first. We typically air-dry the
slides, yes, standing up, overnight, before warming them.
An additional step that often is helpful is to submit pericolonic adipose
tissue with vessels... frequently you can find microscopic lymph nodes/lymphoid
aggregates.
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Hi,
Does anyone out there know if there is a monoclonal anti human NK cell antibody
that is exclusive, ie does not also mark CD8 T cells etc?
Kim
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do a search on the histonet archive
i posted a method quite a few years back
processed and cut eyes (human and piggy) on a daily basis for almost 10
years
go find it - it may help
patience, determination, thicker sections, 2 x pre-float out first on dist
water and then very dilute alcohol to
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