AW: [Histonet] C4d Antibody

Hi César we use mo-a-C4d, clone CL314 (Quidel A213) at 1.25 ug/ml (1:800) for cryosections of kidney biopsies (indirect immunofluorescence), it won't work on FFPE material (at least in our hands). For FFPE, Biomedica's rb-a-C4d (BI-RC4D) works fine at ca. 6 ug/ml (1:30) after HIER in a high pH

[Histonet] Substitutes for Technovit 7100

Hi all, I am lookin for a substitute for Technovit 7100 in our research (currently on fish female gonad). Any help or suggestions about this point would be very very appreciated. Best regards Jorge Tornero IEO - Cádiz- Spain ___ Histonet mailing list

Re: [Histonet] C4d Antibody

We get our from Alpco. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced!

[Histonet] RELIA JOB ALERT Brand New Start-up Specialty Lab in Poughkeepsie NY!!

Hi Histonetters! I hope everyone is having a great day. I have a new opportunity I want to tell you about! I am working with a brand new specialty lab opening in the Poughkeepsie area. They are in need of 2 histotechs 1-junior and 1 senior tech. for full time permanent day shift positions. The

[Histonet] hematoxylin Question

Hello All, How long is hematoxylin (we use Richard-Allen Heme 2) good for after it is poured into a staining container?  The stain gets used so infrequently I would like to keep it as long as I can.  We currently dump it weekly regardless of use.  Thank you.   -Kristen

AW: [Histonet] C4d Antibody

Our's is from Zytomed, a polyclonal rabbit (a12-5000). We work with the benchmark XT, 1:25 with amplifier. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Cesar Francisco Romero Gesendet:

[Histonet] Thanks for all the formalin waste responses

    Hi All I just wanted to say thanks to everyone who has responded to my question regarding formalin waste going down the drain. I appreciate it! Have a great day! Jessica Piche-Grocki, HT(ASCP) ___ Histonet mailing list

RE: [Histonet] hematoxylin Question

What I do Kristen if I use it infrequently is I get a large brown bottle, label it with working hematoxylin. After I use the hematoxylin for whatever I am using it for I pour it in the brown bottle, cap it tightly and store it on the shelf or in a cabinet. Whenever I need it again I get it

[Histonet] IF

Hi everyone, When carrying out IF on frozen sections how long after the cutting the sections should you leave the slides to dry and where before starting the staining procedure? Thanks a mill, V ___ Histonet mailing list

Re: [Histonet] IF

We don't let our unfixed sections air-dry (room temp) long before fixing them - a few minutes? For some people here I let them sit a few minutes then just store them in the -80 for up to a weeks weeks before they fix them and commence with their staining procedure. If the animals were

Re: [Histonet] IF

sorry, that should read for up to a few weeks. --On Tuesday, March 24, 2009 1:00 PM -0400 Merced Leiker lei...@buffalo.edu wrote: We don't let our unfixed sections air-dry (room temp) long before fixing them - a few minutes? For some people here I let them sit a few minutes then just store

[Histonet] Re: IF

Vanessa, The answer of how long is suitable, the answer is it depends. Routinely, many will air dry over night, even over a fan, in order to achieve the best adherence to the glass slide. We have found that we lose immunoreactivity with some antigens if we let it go that long. Our routine has

[Histonet] Reference or service lab that does PDGFR-a staining?

Hi, does anyone know of a reference or service lab that does immuno staining for PDGFR-a,b (platelet derived growth factor receptor alpha and/or beta. Please note my new street address and phone number Tim Morken Technical Support Manager Immunohistochemistry Anatomical Pathology Thermo

RE: [Histonet] Reference or service lab that does PDGFR-a staining?

USLabs performs this test. Jaime -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Tuesday, March 24, 2009 10:39 AM To: histonet Subject: [Histonet] Reference or service lab that does

RE: [Histonet] re: water

Does anyone know of a method to change the pH of tap water without going through a lot of expense? Possibly a filter to put directly on the water line. Thanks, Toni -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on

[Histonet] LabVision line of instrumentation, antibodies and reagents

I was wondering if there are any clinical labs that use the LabVision line of instrumentation, antibodies and reagents. We are a small hospital that does about 30 predilute antibodies and are considering changing from our current vendor/instrument. I would appreciate good, bad experiences. I

[Histonet] RE: Histonet Digest, Vol 64, Issue 42 JCAHO - Universal Protocol (Amy Self)

We do 3 to 4 FNA's on a daily basis and no matter the site thyroid, liver ..Etc we go through the time out procedure every time, yes it may a little bit overkill for a palpable mass or using ultrasound guidance but none the less you must do it just like labeling the lido cane when it is the

[Histonet] re:microtome questions

I'd go for the old AO 820. They can be used by any and everyone and its nearly impossible to break them. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FW: formalin neutralizers

Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: Burton, Lynn Sent: Tue 3/24/2009 10:31 AM To: histonet-bou...@lists.utsouthwestern.edu Subject: formalin neutralizers What is everyone using to neutral their formalin? Who has the best

RE: [Histonet] LabVision line of instrumentation, antibodies and reagents

Hi Rene, I love LabVision products! Hopefully they won't be ruined by Fisher(I would put in names of all acquisitions but it would take too long to type)... Noelle (LabVision cheerleader) Noёlle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services Department of Pathology Laboratory

[Histonet] Re: CD31 anti-mouse on paraffin sections

Hi, Has someone tried an antibody that would detect CD31 on mouse tissues using 10% NBF and not Zinc formalin? Thanks, Lizbeth Kelly, HT(ASCP), Q-IHC Histology Department Human Genome Sciences, Inc. 14200 Shady Grove Road Rockville, MD 20850 240-314-4400 X2325 Fax: 301-354-4176

Re: [Histonet] IF

30 min at room temperature René J. --- On Tue, 3/24/09, Vanessa J. Phelan vjp2...@columbia.edu wrote: From: Vanessa J. Phelan vjp2...@columbia.edu Subject: [Histonet] IF To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, March 24, 2009, 12:17 PM Hi everyone,

[Histonet] California Symposium

The final program is available for the California Society for Histotechnology annual symposium. The symposium is scheduled for May 14-17 in Millbrae. If you are interested I can send you a word document or a pdf file. It will be posted on the CSH website in the next day or so.

Re: [Histonet] hematoxylin Question

We use ours for a few weeks, but we do filter it before use. kristen arvidson arvidsonkris...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 03/24/2009 07:48 AM Please respond to arvidsonkris...@yahoo.com To histonet histonet@lists.utsouthwestern.edu cc Subject [Histonet]

[Histonet] QIHC certificate

Hi, all I have a question about how to QIHC certificate. We have several bilogical scientists work as full-time employee doing IHC staining. But none of them has HT certificate. In this case, can they get QIHC certificate? What kind of courses need they to take? Thank you, Ann Fu Lab

[Histonet] questions re: fixing in general and Histochoice in particular

Hi all. Having a bit of an internal debate here, so I would like to get the opinions of some of you in Histoland, please. Here are the questions: 1.When fixing with 10% NBF, for how long should you fix and what volume ratio of fixative:tissue should you use? 2. At what

Re: [Histonet] questions re: fixing in general and Histochoice in particular

1. Generally 24 hrs for 2-3 mm thick piece. At least a 10:1 formalin:tissue volume. 2. Room temp or 4oC - I've heard debates, and I've heard it doesn't matter, but we do it at 4oC. --On Tuesday, March 24, 2009 4:54 PM -0400 Jacqui Detmar det...@lunenfeld.ca wrote: Hi all. Having a bit

[Histonet] Looking for a CD20 that works on mouse tissue

Hello Histonetters, Have a new project that wants to stain CD20 in mouse samples. One is brain and the other heart. Has anyone done this CD marker in mouse? if so, would you be willing to share with us! Thanks in advance, Colleen Forster U of MN

RE: [Histonet] LabVision line of instrumentation, antibodies and reagents

Hello, We do IF's on renal biopsies and skin. We were getting our antibodies and Protein Block from ThermoShandon which is now Lab vision. We are now not getting our supplies when needed. We have Protein Block on order and were told we would not get any until end of April, which was then

[Histonet] IHC Stainer: Microm HMS 710i

I am looking for help/advice for using our automated immunostainer the Microm HMS 710i .(Our user manual says Richard Allan Sci and the labels on the side say LabVision, we have calls into Thermo's LabVision department but they are unsure if they can assist us) Specifically, one of our problems

RE: [Histonet] FW: formalin neutralizers

This is what we use: Neutralization and Disposal of Formalin Fixative 10% Formalin can be neutralised with sodium bisulfite or concentrated ammonia. The reaction with ammonia results in the formation of hexamethylenetetramine (commonly known as hexamine or methenamine). This can then be safely

RE: [Histonet] QIHC certificate

From the ASCP Board of Registry webpage: www.ascp.org/bor Click on Qualifications Click on IHC There are 3 routes. To summarize, all experience must be within the last 5 years, in a US lab, Canadian lab, or CAP/JC accredited lab: 1. ASCP certified technologist + 6 months full time IHC experience