As a pathologist, I am a strong proponent of ample gross photography in the
cutting room. When I first started in my current place, I thought that not
much gross photography was being done. This has increased in recent years in
our center. I always encourage our residents to take digital gross
phot
like Mike,
we only photograph unusual specimens. Seems photographing specimens has
become less and less important. Kind of like autopsies.
JTT
- Original Message -
From: "Mike Pence"
To: "Steven Joy" ;
Sent: Friday, April 17, 2009 4:20 PM
Subject: RE: [Histonet] Gross Photography
Hello Histonetters,
The Louisiana Society for Histotechnology is pleased to announce the
26th Annual Symposium/Convention:
"Your Histeaux Surplus Package"
June 12 & 13, 2009
at the
Bourbon Orleans Hotel
717 Orleans St.
New Orleans, LA 70117
www.bourbonorleans.com
The LSH block of r
Hello,
I am looking for a recommendation for a new CD15 .. our current clone is
LeuM1 and I would like to know the preferred one out there ... also I am
looking at working up a Troma-1 and I was curious if anyone is doing it
the with DAKO Envision plus system?? Anyone??
Thanks!
Tiana
This email a
I have a tech who is interested in learning to service (PM) microtomes.
Has there been an NSH or regional workshop on this topic recently? Can
anyone suggest a vendor or other program, if not NSH?
First off let me preface this noting that this is my first ever reply,
so hopefully I am doin
I only photograph specimens that are not "routine" type specimens.
Something that you might see only a few times a year or that once in a
lifetime specimen. We also will get request from the surgeon to
photograph a specimen for them at gross.
Mike
-Original Message-
From: histonet-boun...
Is there a range of practice among centers as to what specimens are
photographed at gross, does anyone photograph pretty much all specimens?
Steve Joy, BSc. MLT
Research and Development Technologist
5B2.03 Anatomical Pathology
University of Alberta Hospital
8440-112 st
Edmonton Ab
T6G 2B7
Phone:
Does anyone have any suggestions or a working immunofluorescence protocol
for staining mouse FFPE tissues with HIF-1alpha rabbit polyclonal or Ref-1
rabbit polyclonal? Both from Santa Cruz.
Thank you, Adam
Adam Bazama
baza0...@umn.edu
Lillehei Heart Institute
Histology Microscopy Resear
Hello All,
I have inherited a Biowave DFR-10 (a fancy Ted Pella microwave) but I did not
inherit the owner's manual.
Does anyone have a copy that they can send me?
Thanks and have a nice weekend.
Paula :-)
Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (V
Thank you all for your suggestions! Though, if anyone still has ideas,
please don't consider this thank you as a closing of the subject - I (and
I'm sure, others who are in my situation) would love to hear any and all
variations that work. Now I'm off to cut more tissue so I can run more
stains usi
Hello Histonetters,
The Louisiana Society for Histotechnology is pleased to announce
the 26th Annual Symposium/Convention:
"Your Histeaux Surplus Package"
June 12 & 13, 2009
at the
Bourbon Orleans Hotel
717 Orleans St.
New Orleans, LA 70117
www.bourbonorleans.com
The LSH bl
Jennitfer,
Found this in the archives from "Joe The Toe".
I remember the procedure. 1. Take off the cover slip and soak in xylene
or substitute for 15 minutes. 2. Apply enough Mount Quick to cover
sections and place in an 80 degree oven for 30 minutes or until
hardened. 3. place slide in tiss
Does anyone have any experience in taking a stained H&E slide (non-charged),
working backwards to rehydration, and floating the individual tissue sections
off into a waterbath to be picked up on a charged slide? I can't come up with
any possible way to successfully do this, but one of our Patho
Our laboratory is ISO 17025 certified/accredited. Having said that: Pathology
is not under "scope:" our Chemistry and Microbiology sections are under
"scope." Our certifying (external auditing)agency is AALA (American Association
for Laboratory Accreditation). They have been auditing Chem and Mi
Anybody tried doing IHC for brdu on ffpe rat intestine and skin fixed for up
to 2 years in NBF?
Thank you,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
Does anyone on Histonet use aggrecan ab to label cartilage cells? I have an
ab from Abcam ?ab3778 which is a mouse monoclonal, but they do not give any
dilution recommendations for ffpe tissue IHC or pretreatments.
Thank you,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste
Sara,
This is what we do here with animal brain tissue:
1) Brain should fix for a 24-48 hrs in NBF.
2) They are trimmed in to about 5 mm thick (for embedding cassette).
3) Place cassettes face UP on the ice tray before sectioning (not face
down).
3) The sections are cut at 4 um.
4) Waterbath t
Rich,
There have been workshops at National (NSH), Regional and State meetings on
grossing, but I do not know of any training programs per se.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
-O
I'm not sure if my prior post went through or not. If it did indeed go
through please for give me for posting it again. I didn't get any
responses and my pathologist is really breathing down my back for
anything I can come up with so he can start to train some histotechs.
Thanks again Rich Y
Hi Sara:
I, and it seems others, disagree with the idea that alcoholic formalin
is the best fixative for CNS. Buffered formalin is the fix of choice for
animal and human CNS.
Geoff
Breeden, Sara wrote:
Yes, I'm back. I need the expertise of this group.
I am trying to convince my three
We've found this also. Even 30-60 minutes air drying helps alot with human
brain. We use regular slides and NBF.
Barbara Albert
UCSF Medical Center
San Francisco
From: "Weems, Joyce"
To: "Breeden, Sara" ; histonet@lists.utsouthwestern.edu
Sent: Friday, April
Ditto Joyce.
This works with animal brain too.
PKP
From: "Weems, Joyce"
To: "Breeden, Sara" ; histonet@lists.utsouthwestern.edu
Sent: Friday, April 17, 2009 9:49:49 AM
Subject: RE: [Histonet] CNS Fixation
I have found that brain sections that are air-dried
Sara:
We always fixed human brains and CNS material in NBF and I really would not
like to use a mixed fixative that has 2 different ways of action (coagulation
and cross-linking). The problem can be solved at the sections level (6-8 µm)
and totally draining the water from underneath the section
Sara-
Welcome back! We use 10% NBF also to fix our mouse & rat brains and
spinal cords, and I use Ultrastick slides for sectioning (6 um sections
are standard here, that's what my boss likes). I bake them in a 60
degrees C oven for 30 minutes-overnight (depending on my schedule).
It's rare
I have found that brain sections that are air-dried overnight - then
dried in the oven rarely ever wash off. We fix in 10% NBF. This is human
- not sure if animal would be different.
Best, j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 3034
Yes, I'm back. I need the expertise of this group.
I am trying to convince my three pathologists that fixation in alcoholic
formalin is the best route for whole brain and spinal cord. This has
been an uphill battle and in order to prevent sections from peeling off
during staining, I am still
You really do not need a pH meter for hematoxylin or eosin. Perhaps for water?
René J.
--- On Thu, 4/16/09, Debbie Vigil wrote:
From: Debbie Vigil
Subject: [Histonet] pH meter
To: histonet@lists.utsouthwestern.edu
Date: Thursday, April 16, 2009, 2:30 PM
I am looking for a pH meter for Histolog
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