Hi,
I am trying very hard to get a clear MMA embedded block. I am appreciate
if there is any advice on doing it. I am using 100% MMA (liquid) and 0.5%
Perkadox 16 (powder) to do the embedding. I have tried to embed the sample
in different conditions. However, it seems to be extremely hard to g
Hi Kerry,
I have well bars with wells of 30 x 50 mm 30 x 30 mm which will should handle
your task. I can also custom make larger ones if necessary if you an wait a few
months. I offer all colleagues to first demo the apparatus with no obligation.
If you are not familiar with my sytem visit:
ht
Does anyone use recycled and/or fresh Formula 83 as a clearant on their Peloris
Tissue Processor?? If so, can you tell me what final threshold percentage you
use.
Thanks,
Ann
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Hello Everyone,
In my continuing quest to find out how to finish putting the bulb assembly back
together on my fluorescence microscope I've found out some additional
information.
The power supply and bulb assembly were made by a company called "OptiQuip". I
have called the company, which sti
HAPPY LAB WEEK EVERYONE! HOPE YOU ALL GET TREATED WELL!!!
Christine Tambasco, HT (ASCP)
ALbany Medical Center NY
> To: histonet@lists.utsouthwestern.edu
> From: kerry.l.cr...@gsk.com
> Date: Mon, 20 Apr 2009 18:31:51 -0400
> Subject: [Histonet] Mold for OCT samples
>
> I've had a reque
I've had a request for a mold that is large enough to freeze a cross
section of human jejunum in OCT. Suggestions would be appreciated.
Kerry
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I live in south central WI just south of Madison, WI and just north of
Rockford, Ill. I'm looking for pool or as needed work as an HT or Mohs Tech.
I will consider all work within
a 100 mile radius. Short-term outside of this area. I've done clinical and
research Histology and am experienced
This may be a question for Gayle, or someone else who specializes in
bone. I will be doing a project involving the facial bones of 8
week-old rabbits. We want to look at regeneration of bone in this area.
I have some experience with BRDU in mouse tissues.
My questions are:
1.) Will decalcificat
Good Afternoon, Anyone having trouble with fiber contamination from
disposable cytofunnels filter? Sometimes it is worse than others and it
is interferring with our silver stains to the point where they are
unreadable. We used to buy the individually wrapped funnels but,
recently we have been us
Animal brain is the same. IF you aior dry overnight and then into the
oven before starting your stains you will not lose sections.
Colleen Forster
U of MN
Barbara Albert wrote:
We've found this also. Even 30-60 minutes air drying helps alot with human
brain. We use regular slides and NBF.
I'm trying to find a source for protease XIV other than Sigma. Any suggestions?
Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007
Patsy,
If the animal was treated with BrdU initially, then yes I have. Unfortunately
All of my papers and reference materials are boxed up at the moment (preparing
to move) but I have done this procedure so many times that I can pretty much
reconstruct it from memory.
1) Hydrate slides to dd
Mary:
The averages are 25 blocks/hour sectioning and 60 blocks/hour embedding. Under
separate cover I am sending productivity values for other tasks.
Another thing is what you are expecting of the multi-tasking,
take-care-of-everything person you are describing. That is a special situation
and t
Imelda,
Chlorantine fast red 5B, AKA direct red 81 and Sirius red 4B, CI 28160 is
listed by VWR International UK(tel 800-932-5000).
Bryan
- Original Message -
From: "McGonnell, Imelda Mary"
To:
Sent: Monday, April 20, 2009 9:38 AM
Subject: [Histonet] suppliers of chlorantine fast
I am working on developing bone in the chick embryo and have a dwindling
stock of chlorantine fast red 5B solution, which is a good marker of
developing bone in histological sections. Does anyone know of a current
supplier of this chemical, I am finding it very hard to find one.
Many thanks
Imelda
We are fortunate to have a wonderful gross photography set up. Our
residents take many pictures. The photos are downloaded and kept in
our LIS system. One nice thing for taking photos is for the mapping of
bone tumors.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas C
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