[Histonet] Mounting sections, rolling sections and peeling off sections

Yes this is a very common approach... we experience difficulty partly because we are cutting thin sections on large samples from wounded tissue... the wounded tissue is a bit more fragile; also, if the section is large, it is very hard to use the small brush to hold it during cutting... in the

Re: [Histonet] Floor materials

Vinyl floors can be almost lethally slippery if wax gets on them its ok if staff wear flat shoes but woe betide someone coming in with heels! On 4/28/09, Feher, Stephen sfe...@cmc-nh.org wrote: We're building a pathology lab and we're at project phase where flooring materials is being

[Histonet] UC Berkeley students in need of help

Fellow Histoneters, Can you spare less 2 minutes of your time to help out a group of 4 MBA students at UC Berkeley? We are conducting market research, by way of a simple online survey, for a class project. Our focus is on ways in which labs can cut significant costs on reagents and

Re: [Histonet] Floor materials

I know they are expensive, but I think that epoxy floors are best in any kind of lab that uses solvents.  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: louise renton louise.ren...@gmail.com To: Histonet@lists.utsouthwestern.edu Sent: Wednesday, April 29,

RE: [Histonet] Rolling sections

Jennifer, It sounds like the sections are a little thick. Have you tried just barely blowing on the tissue with your breath to warm the OCT? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dearolf,

RE: [Histonet] Destaining HE slides

Anything mildly basic will quickly destain eosin. Running tap water will do it, usually in 10 minutes or so. 0.1% NaOH or KOH in water or in 70% ethanol will do it quickly. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Destaining HE slides

Thanks to everybody for their destaining tips! -Kathleen Monfils, Paul wrote: Anything mildly basic will quickly destain eosin. Running tap water will do it, usually in 10 minutes or so. 0.1% NaOH or KOH in water or in 70% ethanol will do it quickly.

RE: [Histonet] Rolling sections

May I suggest trying the following. I too have experienced cryosectioned slices rolling up. 1) try cutting sections at -20 instead of -25 (it may be too cold) 2) try cutting sections at 6 microns instead of 8-12 microns 3) allow some frost to build up on the metal plate surface and gently with

Re: [Histonet] Rolling sections

If your cryostat is set up correctly (ie, the roll plate isn't causing the rolling up), then I would try 100% EtOH instead of 70%, I have no idea why it works but sometimes, it does. Also, when our cryostat starts doing this, I just clean the blade and the knife and walk away for a few

[Histonet] Re: mid-atlantic regional histo meeting

Histonetter's: Looking to get those needed CEU's or just want to learn something new? The states of PA, MD, NJ and DE are hosting a regional one day event on Saturday,May 16th, 2009 ...for Virginians and state and NSH members, at the Old Dominion University Higher Education Center, in Virginia

[Histonet] Colorado Society of Histotechnology - Meeting May 8th 9th in Colorado Springs

Hi, The Colorado Society of Histotechnology (CSH) meeting will be held at the Cheyenne Mountain Resort in Colorado Springs, May 8th and 9th, 2009. If you are interested in attending the meeting and have not registered, please visit our website at http://www.coloradohisto.org/2009/meeting.htm.