If your cryostat is set up correctly (ie, the roll plate isn't causing the rolling up), then I would try 100% EtOH instead of 70%, I have no idea why it works but sometimes, it does. Also, when our cryostat starts doing this, I just clean the blade and the knife and walk away for a few minutes--but our rolling-up is due to static electricity. You can also ground the blade by touching it with something metal. I wouldn't change your section thickness--rolling up has nothing to do with that if you've got your cryostat set right. Also don't breathe on your sections--that's not a particularly scientific way to get what you need, and you might need RNAse free sections in the future (RNAse free, your breath is not.)
Emily prometheus, thief of light, giver of light, bound by the gods, must have been a book. -mark danielewski, house of leaves > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _________________________________________________ > Confidential information. Unauthorized use or disclosure prohibited. > Refer http://www.singhealth.com.sg/ContactUs/#Disclaimer > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet