Hi Wladislav,
this technique is called FICTION. I'm sure, that you have done a literature
search on this.
If you need pepsin for your FISH depends on the fixation-status of the
tissue for permeabilization and digestion of the histon-proteins. I guess
formaldehyde fixed tissue cannot do without it.
I've just found a publication, where the IF is done before FISH. Look at
this:
http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1770804blobtype=pdf
bye Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
Jason:
In my opinion, any recommendation to re-use a retrieval buffer just isn't
'good science'. The problem is that one cannot know, with any certainty,
when a solution loses it effectiveness. The primary reason that the re-use
approach has been advocated is because this device consumes a
We were having issues with placenta contamination aswell. Our Dr.s now wrap the
placenta sections in tissue paper and this seems to have cut down on the
problem.
Sheila Adey HT MLT Port Huron Hospital Michigan
Date: Fri, 1 May 2009 15:41:37 -0500
From: mpe...@grhs.net
To:
That processor spins the tissue around and causes much more agitation than
with other tissue processors. Tissue gets out of the cassettes easier. It
makes for faster processing, but you have to use mesh cassettes or wrap
placenta and other small friable tissues.
Mark
On Fri, May 1, 2009 at 1:41
I don't know whether you are decalcifying tissue or not, but I have had
similar problems with tissue that was over-decalcified. It was particularly
bad when decalcified with commercial decalcifying agent which contained
formic along with several chelating agents. The end result was tissue that
This is a late response, but I have IHC staining for E. histolytica on
formalin-fixed, parafffin-embedded tissue in my laboratory.
Richard
Richard W. Cartun, Ph.D.
Director, Histology Immunopathology
Director, Biospecimens
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour
