[Histonet] Destain Hematoxylon

Hi, I want to destain hematoxylin (Shandon's Gill II). How to do that? Thanks in advance, V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Which kind of pre-coated slides is good for brain sections?

Just want to buy some slides for our brain sections. Pre-fixed before cryostat section. normally 10 um -40 um. Thanks very much ! s/#Disclaimer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Journal of histotechnology paper searching

hello,   I was sarching for a paper from Journal of histotechnology. Unfortunately the paper doesn't show up on medline and I can't seem to  find an archive for J Histotech that goes back that far. I wish somebody could help me .  The paper is:  Okia, Zelda, Tihan, Tarik, Kane, Philip.

Re: [Histonet] Journal of histotechnology paper searching

If you are a member of the NSH you can ask the article from them and they will send you a pdf file René J. --- On Mon, 5/4/09, 伍治 woods1...@yahoo.cn wrote: From: 伍治 woods1...@yahoo.cn Subject: [Histonet] Journal of histotechnology paper searching To: Histonet@lists.utsouthwestern.edu Date:

[Histonet] 2009 Louisiana Society for Histotechnology Annual Symposium/Convention

Hello Histonetters, The Louisiana Society for Histotechnology is pleased to announce the 26th Annual Symposium/Convention: Your Histeaux Surplus Package June

RE: [Histonet] Oil red o mounting media

We use Crystalmount. You don't even have to coverslip. Just make sure you evenly distribute the mountant on the slide and let it dry. We usually put it on a hot plate for a bit. This leaves a hardened shell around the tissue. The slides can be read like this too. If you really need a coverslip,

RE: [Histonet] xylene

We had a paraffin plug in the waste container, which caused a malfunction of the unit and contamination of our reagents. We paid for CBG to come out and perform and thorough PM before we used it again. We have had the unit for about 7 or 8 years and this is the first time this happened. Laurie

[Histonet] ER/PR scoring guidelines

Can someone provide me with the guidelines (Canadian) for scoring ER/PR and what the preferred antibody is for determining this. I see there are several clones. Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] STP420

That makes sense. Know I can see where the processor may be responsible for the carry over. This is a good thing to know if and when looking for new tissue processors. Mike -Original Message- From: Mark Tarango [mailto:marktara...@gmail.com] Sent: Saturday, May

[Histonet] Lab Safety

Would anyone be willing to share what they do for lab safety as far as teaching, and keeping up with what is requested by CAP? Since I have chemicals, I have been nominated to be in charge of the Chemical Hygiene manual which now consists of all training for the lab and so forth. What are you

[Histonet] XXXL GLOVES

Hi All, We have a morgue attendent who does not fit into the XXL gloves. Does anyone know a vendor who can provide XXXL GLOVES latex (powder free) prefered, but will take any other brand too. I do really appreciate the help! Thanks Nirmala Srishan Holy Name Hospital Teaneck NJ 07666

[Histonet] re: destain hematoxylin

1) Acid alcohol: I use several drops of concentrated HCL in 95% ethanol (1% HCL in 70% alcohol well too). Simply dip your rinsed slides (or sections) into solution a couple of times, rinse and check them... a dip in water with about 10drops of ammonia should follow as well... 2) Saturated picric

[Histonet] direct-conjugates for IHC

Hello everyone! I work with a scientist who insists on using primary antibodies directly conjugated with FITC or Texas Red for IHC. In my past experience these directly conjugated antibodies didn't give a strong enough signal for use in IHC (I've seen them used only for FACS analysis). I

Re: [Histonet] (canine) eosinophil IHC

Any good HE stain will reveal the eosinophils. René J. --- On Mon, 5/4/09, Jan Shivers shive...@umn.edu wrote: From: Jan Shivers shive...@umn.edu Subject: [Histonet] (canine) eosinophil IHC To: histonet histonet@lists.utsouthwestern.edu Date: Monday, May 4, 2009, 3:41 PM Hello all, I'm asking

Re: [Histonet] Destain Hematoxylon

Acid alcohol. It may not remove all of it but it will remove the majority. On Mon, May 4, 2009 at 5:32 AM, V. Neubert histonet.nos...@vneubert.comwrote: Hi, I want to destain hematoxylin (Shandon's Gill II). How to do that? Thanks in advance, V. Neubert

Re: [Histonet] direct-conjugates for IHC

Jennifer, In the past, upon occasion when need arose, I'd use directly conjugated primary antibodies.  Mainly biotin or dig or peroxidase but also FITC directly to primary.  Then can look at it fluorescently or come back with an anti-FITC of some kind (several are good). Used these for my