RE: [Histonet] Gram AFB staining FNA smears

2009-05-07 Thread Kemlo Rogerson
Agree; I guess the difference is that FNAC and Micro samples haven't been subjected to processing like the tissue block sections. The tissue stains have been shown to work on something that has been fixed, dehydrated, set in hot wax, cut, rehydrated and then stained. The FNAC and Micro samples

Re: [Histonet] wright-giemsa stain on tissue

2009-05-07 Thread Rene J Buesa
Medea: Under separate cover I am sending an article I wrote on Giemsa for FFPE tissues. René J. --- On Wed, 5/6/09, Medea McGraw mmcg...@phenopath.com wrote: From: Medea McGraw mmcg...@phenopath.com Subject: [Histonet] wright-giemsa stain on tissue To: histonet@lists.utsouthwestern.edu Date:

[Histonet] Microtome Alignment

2009-05-07 Thread kristen arvidson
Does anyone know where I can an alignment device? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Microtome Alignment

2009-05-07 Thread Bartlett, Jeanine (CDC/CCID/NCZVED)
Newcomer sells them. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Thursday,

Re: [Histonet] Microtome Alignment

2009-05-07 Thread Akemi Allison-Tacha
David Davis from CO designed an alignment device and can be purchased from Newcomer supply or I think his e-mail is still the following: davidda...@uchsc.edu Good Luck, Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los

[Histonet] New HTL Program!

2009-05-07 Thread Kimberly Feaster
West Virginia University School of Medicine Histotechnology Program is now accepting applications for fall 2009 enrollment! The WVU Histotechnology Program is offered as an area of emphasis within the Medical Laboratory Science major. Students graduate with a Bachelor’s of Science degree in

[Histonet] Cryo question

2009-05-07 Thread Jennifer S Finke
To whom it may concern: I have a question about OTC emb= edded tissues. They were embedded sometime ago and kept at -20C. Is there a= way to re-embed, and re-hydrate the tissue? Thank you, Jennifer Finke-Dwyer Department of Pharmacology and Experi= mental

RE: [Histonet] New HTL Program!

2009-05-07 Thread Kemlo Rogerson
Congratulations from the UK. As you know we've had this in place for many years and resulted in the profession having a higher profile; I hope the rest will follow for you. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension

[Histonet] Request for Staining Advice: Rabbit Calvaria underlying Cerebral Hemishpere

2009-05-07 Thread Sean McBride
Colleagues, I have been charged with the task of evaluating an hydroxyapatite coated biomaterial implanted in the calvaria of a rabbit model. The calvaria and cerebral hemisphere were excised en bloc, and the objective of the experiment is to assess not only new bone formation and

RE: [Histonet] Request for Staining Advice: Rabbit Calvaria underlying Cerebral Hemishpere

2009-05-07 Thread Shakun Aswani
Try Goldner's Trichrome Stain. I have protocol if you want -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sean McBride Sent: Thursday, May 07, 2009 8:05 AM To: histonet@lists.utsouthwestern.edu Subject:

RE: [Histonet] Microtome Alignment

2009-05-07 Thread Bernice Frederick
Microm sells a device called a histocollimeter, however, it only attaches to their microtomes to my knowledge. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original

RE: [Histonet] Microtome Alignment

2009-05-07 Thread Jodie Robertson
Newcomer Supply. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Thursday, May 07, 2009 6:24 AM To: histonet Subject:

[Histonet] how to unregister from histo-net

2009-05-07 Thread koswalt
Could anyone help me. I am trying to un-listg with the histo-net services. Please let me know. Thanks, Kim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Remove from e-mail

2009-05-07 Thread koswalt
Please remove me from your e-mail lists. Thank You, Kim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] NADPH-diaphorase

2009-05-07 Thread Breisch, Eric
Hi, hoping that someone might be able to share with us where they purchase NADPH-diaphorase for the NADPH stain. Thank you. EAB Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy

Re: [Histonet] Microtome Alignment

2009-05-07 Thread Victor Tobias
I just went to Newcomer Supply to see what you get for $700, not much. Go to Ace Hardware and get each employee one of these. It will work just as well and works on any microtome. Buy your staff lunch with what you save.

Re: [Histonet] NADPH-diaphorase

2009-05-07 Thread Amy Porter
Try Sigma-Aldrich. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: port...@msu.edu Web: www.humanpathology.msu.edu - Original

RE: [Histonet] Microtome Alignment

2009-05-07 Thread Martin, Gary
As an old carpenter I quickly adapted a speed square to do pretty much the same thing as the $775.00 unit or the torpedo level. The speed square has the advantage of squaring the block holder to a true flat surface (the base of the microtome) it works quite well ... it is positive and quick. See

Re: [Histonet] FW: prover' pojaluista

2009-05-07 Thread Rene J Buesa
Strictly speaking, IHC reactions do not depend on water temperature temperature. What you describe does not include dist. water between the IHC steps therefore the water temp. should not be affecting your reactions. If you use dist water after HIER you have to remember placing the slides into

Make a Tiny URL of that thing...RE: [Histonet] Microtome Alignment

2009-05-07 Thread Morken, Tim
Victor wrote: Go to Ace Hardware and get each employee one of these... http://www.acehardware.com/product/index.jsp?productId=1286367cp=fbc=1pg=3kw=levellmdn=Price+Rangefbn=StorePrice%7CUnder+%2425.00fr=StorePrice%2FACE%2F%2F2500parentPage=searchsearchId=37640061873 You all

[Histonet] pinning specimens for fixation

2009-05-07 Thread Bryan Watson
We have a surgeon who likes to send us specimens that are pinned down to a board of some sort. He uses whatever makeshift board he can find and he wants us to order something for that purpose. Does anybody use anything like this something strong enough to hold a specimen pinned down without the

[Histonet] Holding Cell Lines in Methanol

2009-05-07 Thread secash
We are doing a 5 day Hypoxia Chamber Experiment on Cancer Cell Lines Slides. They are fixed in methanol at -20c for 20 minutes when they come out. If I cannot do the experiment at that time (due to a funeral), can these slides be stored in the methanol at -20c overnight without it adversely

RE: [Histonet] pinning specimens for fixation

2009-05-07 Thread Garcia, Lori, Sr. Scientist
Hi Bryan, Mopec makes disposable cutting boards that can be cut to the right size. Here is the link: http://www.mopec.com/product/626/small_disposable_cutting_boards/ Also, thick cork board works very well. Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] Gram AFB staining FNA smears

2009-05-07 Thread Maxim Peshkov
Angela: We stains our cytology slides for Gram and AFB with the same reagents as histology. Only misses dewax and final dehydration, clearing and coverslip steps. Results are like both, the pathologists and cytopathologist. Maxim Peshkov, Russia, Taganrog.

Re: [Histonet] pinning specimens for fixation

2009-05-07 Thread Bryan Llewellyn
Try coroplast panels from your local home renovation/hardware store and cut to the size you want with a utility knife. Also, make sure that the surgeon uses plastic pins, not steel, to avoid rust. Since they will probably float, turn them over so the tissue is in the fixative. Bryan -

[Histonet] disposal of body parts

2009-05-07 Thread Carol Bryant
Hello Histonetters! I work in a small multispecialty clinic laboratory where the majority of our specimens are derm, gi biopsies, etc. Today we had an amputated finger sent for gross only from our ambulatory surgery center and the patient wishes to have it back. Does CAP have any specific

RE: [Histonet] NADPH-diaphorase

2009-05-07 Thread Tony Henwood
Eab, We purchase our NADH (reduced form) Cat No. N8129 from Sigma-aldrich Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street,

RE: [Histonet] Turnbull's Blue

2009-05-07 Thread Lee Peggy Wenk
Turnball blue (i.e., Schmorl stain) demonstrates argentaffinic components, such as melanin or argentaffin cells in the GI tract. Prussian blue (i.e., the iron stain) demonstrates iron (hemosiderin). What are you trying to demonstrate? Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospitals Royal Oak,