Hi Mark,Mouse CD4, clone 4B12 and Rabbit monoclonal CD8, clone SP16 is a good
choice for your triple. Lots of success!Chris Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands Date: Fri, 22 May 2009 10:22:14 -0700
From:
Hi alle
i am writing to see if you can provide me any useful protocol in removing the
calcium from whole rat skull fixed in PFA for frozen sections later on.
I noticed there is use of 12% formic acid mixed in 6.25% formalin;
or 4% HCl mixed with 8% formic acid;
or,
Hello,
Anyone out there tried IHC/DAB on sections and thereafter stained with
luxol fast blue-cresyl violet? I'm looking at neurogensis in the brain
(optic tectum) of fish and want to be able to get a good idea of which
OT layers the DAB+ nuclei are lying in. Any other suggestions for a
Hello,
We are accepting bids from EM labs for the processing and imaging of
10 rat brains. We will need 20-25 images (cortex and possibly
hippocampus) from each brain. Brains will be perfusion fixed, stored
in post-fixative solution, and shipped to the EM lab for secondary
fixation, processing,
Anjan,
I am more of a fan of steamer or waterbath than pressure cooker, in
my= hands at altitude the pressure cooker can be very harsh and make
my tissue= s fall off the slide or get chewed up badly. I know the
pressure is s= upposed to be controled by the cooker but in
