[Histonet] Jerry sent you a private message on Tagged :)

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Re: [Histonet] off e-mail list new account

Webmaster! A beer! "Canned food is dead foodhere kids can't study. They're going blind. Mothers are out in pants showing themselves off when they ought to be at home cooking fresh vegetables." --Charles Atlas, Saga magazine interview, 1964 On Fri, Jun 5, 2009 at 4:43 PM, Peter Carroll wro

Re: [Histonet] immunohistochemistry

I second Mr. Troutman's recommendation using silane slides, = but air dry overnight. Once the slides have been baked and paraffin re= moved, place the slides in citrate buffer and heat them in a closed plastic coplin jar for 12 hours at 75 degrees C. Some epitopes can be

RE: [Histonet] immunohistochemistry

Good advice since actively boiling buffer puts mechanical stresses on section, with bone more easily loosened by this action. It may help to preheat the buffer to 95C before slide/section immersion into retrieval buffer. A friend who is an IHC expert always did this, plus she used plastic coplin ja

Re: [Histonet] immunohistochemistry

I would recommend the following: Citrate for 35 min at 95 deg. Cool down for 10 min. (This is our protocol.) Be sure you are using charged slides and I would let them air dry for at least 1 hour before heating and deparaffinizing. That might help, too. Unfortunately, I don't think there i

Re: [Histonet] Frozen Artifacts

Hi Nicole, You must tell the list what the artifacts are before we can help you. You're note is a little too vague. Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center (CRIC) 3350 La Jolla Village Dr., MC151 San Diego, CA 921

Re: [Histonet] off e-mail list new account

Webmaster, please be kind enough to serve us all tea and snacks, too. It's Friday afternoon! ;) James Aflleje wrote: Webmaster please be kind to take me off your e-mail address. Thanks, James Aflleje HTL ASCP, Director of Laboratory, INTEGRATED-PATHOLOGY ___

[Histonet] off e-mail list new account

Webmaster please be kind to take me off your e-mail address. Thanks, James Aflleje HTL ASCP, Director of Laboratory, INTEGRATED-PATHOLOGY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

[Histonet] Frozen Artifacts

Hello Histonet... I am using frozen brain sections and I have terrible frozen artifacts. I receive the tissue from a brain bank so I have no control over the method of freezing. Is there ANYTHING that I can do at this point to at least reduce these artifacts? I've tried formalin fixation post-c

[Histonet] Re: Toludine blue stain

You mention plastic? Which one are you using? I have more than one toluidine blue staining method depending on the plastic I am using. The T blue/sodium borate was used mostly for EM resins, but not for methyl or glycol methacrylates. Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT _

Re: [Histonet] Toluidine blue staining

To get consistent staining you must standardize your procedure. I make 1% aqueous Tol. Blue and 2% aqueous borax (sodium tetraborate) in separate containers. Each day I mix equal parts of each solution so I always use fresh stain. My final conc. of stain is 0.5%, doubling to 1% makes no differe

[Histonet] re: Cut off times for tissue to be processed

We try to start our tissue processors at 5:30 p.m. every night (our processors for large specimens have 4 hours of formalin on them). I say "try" because sometimes residents or fellows bring late specimens down to our grossing room from the OR where they have already been grossed which results

[Histonet] Toluidine blue staining

Hello. Our lab has had highly variable results using toluidine blue to stain 1.5 micron thick cross sections of plastic embedded nerve tissue from both mouse and human nerve biopsy. It seems like the stain is darker in the biopsy tissue than the mouse but both sometimes stain equally poorly. We ha

[Histonet] re: Cut off times for tissue to be processed

I am trying to develop a policy for cut off times for putting tissue into the processor and wanted find out if anyone out there would like to share what they have. Please email me directly-thank you in advance. Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center 5666 East St

Re: [Histonet] Lab Water Quality

18.2 mega-ohms of electrical resistance. --On Friday, June 05, 2009 12:19 PM -0400 Veterinary Services Laboratory wrote: I have one more question, what is the best electrical conductivity for lab water. We use softened reverse osmosis deionized water and consistently get an EC value of ~ 2.

[Histonet] Lab Water Quality

I have one more question, what is the best electrical conductivity for lab water. We use softened reverse osmosis deionized water and consistently get an EC value of ~ 2. is this okay, if not, are their any suggestions. Thanks Sharon Ms. Sharon Drayton Veterinary Services Laboratory

Re: [Histonet] Storage of tissues in PFA before dehydrationandembedding

I would store in 30% ethanol instead of 70%. Mouse tissues are so brittle after dehydration and infiltration so keep the dehydration step in 70% EtOH the length of time indicated by your processor (usually 30 minutes or so). Also mouse skin is normally very thin, so if you fix for 4h at RT it sh

[Histonet] brilliant blue & safranin O

Has anyone done this stain for chromatin and spindles in cell culture? My results in the search for this technique are very sketchy. Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 kath

Re: [Histonet] immunohistochemistry

Does the marrow stay on but the cortical bone comes off? Are they paraffin sections? What is your decal method? Do you use coated slides? Do you "melt" your sections onto the slides before deparaffinization? Even so, I suspect the reason is your antigen retrieval solution is too hot and too vigor

[Histonet] Leica Peloris and ASP300

Hello-I would appreciate input and comments from anyone using the Leica Peloris or the ASP 300, Thanks! Regards, Kate Kathleen Caleri, BS, MLT, HT (ASCP) Histology Lab Supervisor Pathology Roswell Park Cancer Institute (716) 845-1329 "Weeds are flowers too, once you get to know them."

RE: [Histonet] Cresyl Violet Counterstain of LFB

Some thoughts: 1. Are you using cresyl violet, or is it cresyl violet acetate, formerly known as cresyl echt violet. It should be the cresyl violet acetate. 2. 5 minutes may not be long enough. We use 1 hour. 3. After differentiation, the only things that should be violet are the nuclei of glial