Sakura Tissue Tek VIP5!!
I have 2 of these sturdy workhorses and am very happy
Have been associated with VIP5's for as long as they have been around -
wonderful machines!
AnnieinAbuDhabi
aka.Annie out of Africa
2009/8/5 Igor Deyneko igor.deyn...@gmail.com
Dear Histonetters!
I need your advice
Greetings!
We have been using Clear-Rite 3 here at our lab, and we are happy with
the product. Our supplier says it will be on back-order for some time
now. Our crack supply folks are looking for another source. I'm taking
another route to find out what products out there are comparable.
Are
Bill:
Xylene substitutes are not all pretty much the same. There are d-Limonene
derivatives (something to stay absolutely away from) and alkane derivatives
with many known and unknown constituents.
Since you are about to change and not have any preference, your best option
both in processing
I would contact Anatech Ltd. they have a substitute Pro-Par Clearant
www.anatechltdusa.com - they are all very knowledgeable and would be able to
help you I am sure. I don't use substitutes in my lab at this time, however
I would contact them if I were ready to make the switch.
Amy S.
Bill,
I have used Formula 83 from CBG Biotech for several years now and like it.
There is no strong odor, dries fast and is recyclable.
http://www.cbgbiotech.com
Linda
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937)
Sorry that is incorrect as companies also offer a substitute that is an
isopar derivative that is not d-limonene and has almost no order. I have
used for several years and is very good. A number of suppliers offer it.
The isopar product is a petroleum distillate and is a known product although
From personal experience some possible solutions:
* Infiltration issues ( is your paraffin fresh, carry over on
reagents)
* Blocks being too warm or too wet
* Once had this issue when cleaning of the processor wasn't being
properly followed.
Hope this helps a bit.
I use Formula 83 from CBG. I used Clear-Rite for quite a few years, but
Formula 83 works as close to Xylene as I have found.
Joyce Cline, H.T. (ASCP)
Technical Specialist
Hagerstown Medical Lab.
301-665-4980
fax 301-665-4941
-Original Message-
From:
Hi Rene,
Why do you not like the d-limonene solvents?
I work with them and the results seem fine. I have to mention that I use an
AutoTechnicon Duo and cannot place it in a fume hood to vent the xylene
vapors. Without proper ventilation the safety people willnot allow the use of
Xylenen.
We also use d-limonene (sold as CitriSolv). We used Hemo-De before. I also
would be curious you (and others) do not like d-limonene.
Peggy
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Va Paula
The last time I was around a citrus based clearing agent was in the 80's
and it gave me severe headaches. I didn't have any problems with it's
performance, just the health aspects. I was using a closed processor at
the time, but no hood..
Victor
Victor Tobias
Clinical Applications Analyst
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of
zippedinsunsh...@aol.com
Sent: Wednesday, August 05, 2009 2:09 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] please
My answer is in the attached article I just published. Please read the
d-Limonene discussion seciton.
René J.
--- On Wed, 8/5/09, Sherwood, Margaret msherw...@partners.org wrote:
From: Sherwood, Margaret msherw...@partners.org
Subject: RE: [Histonet] Clear-Rite 3
To: Va Paula Sicurello
Kathy Maddox in Lake Charles, Louisiana asks:
I need to buy some Helicobacter Pylori special stain control slides. The ones
I have previously bought were from rat tissue and our pathologists don't like
them. Can anyone suggest a company that I can purchase them from?
Most of the pathology
William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good
Samaritan Hospital, Kearney, Nebraska, notes that Clear-Rite 3 is at
least temporarily unavailable, and he is looking for a substitute.
(Anybody know what's going on with Clear-Rite 3?)
Clear-Rite 3 is an aliphatic (rather than a
Classification: UNCLASSIFIED
Caveats: NONE
I have 2 Leica ASP 300 tissue processors. I use Thermo Scientific Shandon
Xylene Substitute (formally known as Histosolv). I have used this for many
years with excellent results. I have never had any problems using this in the
processor. We had 2
Hi Teri,
We have been discussing this option and left it at that.
I have noticed that the livers, with more lipids look much closer to the
textbook images.
Michelle,
Could it be what you are seeing is normal healthy (actively feeding) liver in
mice and the missing cytoplasm is where the
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