Hi, I am doing MMA plastic embedding and have a problem with getting a
cloudy embedded sample. I am using MMA plus perkadox 16. Not too sure
whether the cloudiness is caused by perkadox 16. I would actually like to
try to do the embedding using MMA and benzoyl peroxide. I would appreciate
if
Hi all,
I'm trying to do some double labelling to show that a protein resides in the ER
but I'm having some trouble getting nice reticulated ER staining in fixed
cells. So far I have tried fixing in 4% PFA (15min on ice) followed by
permeabilization with Tritonx100 (0.25%; 10min; room temp) or
Hello Histo-Netters,
We have an HT job opening here in Longmont Colorado, located north of Denver
and just NE of Boulder. Please apply through the website. I have attached the
link. All new and experianced HT's please apply. Also please add my name to
your application so we can track the
Hello Everybody,
I was wondering what type of institutional support y'all have at your various
institutions. I work for a research foundation and they want to drop my salary
and have the researchers grants pay my salary and almost everything else. I am
a fee-for-service lab and the CEO wants
Hello Everybody,
I was wondering what type of institutional support y'all have at your various
institutions. I work for a research foundation and they want to drop my salary
and have the researchers grants pay my salary and almost everything else. I am
a fee-for-service lab and the CEO
Hi-
I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm
able to find at this point are the metal molds with the 'wings' on them.
Does anyone know a source? With all the mergers etc it's been a little
challenging.
Thanks in advance
Vikki
Try EM Sciences the distribute alot of the unique old type histology
supplies.
Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg. Rm #2133
East Lansing, MI 48824-3320
Phone: (517) 884-5026
Fax: (517) 432-1368
Email:
My boss has thought up a new project for me so I need to know what I
need to know about Mycoplasma bovis for IHC. He wants me to cost out
the procedure. I normally do only TWO IHCs - CWD and BVD - so I'll be
charting new mental territory with this one. Can anyone guide me on
where to start
Hi there,
Check out this database. It contains a contact list for the labs that are
currently doing the Myco bovis IHC.
http://ihc.sdstate.org/
-J
Jenee S. Odani, D.V.M., Dipl. ACVP
Veterinary Medical Officer
Hawaii State Veterinary Laboratory/DAI
99-941 Halawa Valley Street, Aiea, HI,
Dear Vikki,
Sakura still carries the metal base molds that are used with Embedding
Rings. They do not have the wings that the ones for use with the Unicassettes
have. Their Sakura part numbers begin from 4121 and higher. If you use the
electronic catalog on Sakura's website
Does anyone out in histology have any documentation from CMS, ASCP, or NSH that
breaks down the job duties or areas for what is covered under Tech
and professional part of the CPT billing codes. For example is Grossing part of
the a profee or tech part of the bill.
Rick Garnhart HT(ASCP)
Hi all,
In my HE staining I always used 1% acid alcohol. (Working with conc. HCl is
not fun!)
There is the Orth's solution available already made, but it is 3%. Do you use
that? How do you use it?
What do you use in your regulat HE stain? I am using it on an old Jung
AUTOSTAINER.
Michelle
Peggy,
If the cement you mention is or similar to PMMA, then you will not be able to
cut thin sections using the sliding/sledge microtome. If I was you, I would not
decal it at all as it will only make the bone less dense than the cement and
will definitely waste your time if you embed it
Maria asks about fetal demise placenta:
We gross in all fetal demise placenta (usually four blocks).If the clinicians
desire genetics, they take?samples from the fetus and the placenta up on the
floor. (There is only a three day window for growing fibroblasts).
The fetus gets a gross only
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