Re: [Histonet] COX-2

2009-08-29 Thread Richard Cartun
It was in USA Today, but will be published in this week's issue of JAMA. RWC Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860)

Re: [Histonet] h.pylori silver staining

2009-08-29 Thread Rene J Buesa
Shahram: Under separate cover I am sending you the procedure. René J. --- On Sat, 8/29/09, Shahram Sabeti sabeti_shah...@yahoo.com wrote: From: Shahram Sabeti sabeti_shah...@yahoo.com Subject: [Histonet] h.pylori silver staining To: histonet@lists.utsouthwestern.edu Date: Saturday, August 29,

RE: SPAM-LOW: Re: [Histonet] COX-2

2009-08-29 Thread Patsy Ruegg
Richard, I did a lot of work on cox2 a while ago on ffpe tissue and I was not ever able to get results I was satisfied with. I would be really interested in this as well, sometimes it just takes finding the right clone and the right pretreatment, I am not there yet with cox2 even though several

RE: SPAM-LOW: [Histonet] Best IHC Stainer

2009-08-29 Thread Patsy Ruegg
I agree with you Amos, but some instruments are more friendly to using other's antibodies and detections than others. In my opinion the Dako autostainer, Leica Bond and the BC IHC autostainers are very good open systems, and even some of them are better at supporting animal and human research.

[Histonet] (no subject)

2009-08-29 Thread Aazath Raj
Dear All, we have got a new leica multistainer ST 5020.we are using it for both HE and as well as PAP.Out of 34 station i have only 15 station for HE.Is anybody having a Staining protocol for HE which fits in 15 station with 2 water wash bath. Aazath Technical Officer Apollo

AW: [Histonet] (no subject)

2009-08-29 Thread Gudrun Lang
Hi Aazath, This is our HE protocol, it's for 16 stations. And I'm interested in your PAP-protocol. Would you be so nice and send it to me? 25 min oven 2 x 5 min Xylol-substitute 1 x 1 min 96% 1 x 1 min 80% 1 x 1 min 50% 1 x 1 min dest. water 1 x 10 min Mayers Hemalaun (exact) 1 x 5 min tapwater

RE: [Histonet] h.pylori silver staining

2009-08-29 Thread WILLIAM DESALVO
A Warthin-Starry procedure works great. The kit and procedure on the DAKO Artisan is fast, reliable and provides good results. William DeSalvo, B.S., HTL(ASCP) Date: Sat, 29 Aug 2009 07:32:10 -0700 From: rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; sabeti_shah...@yahoo.com

Re: [Histonet] EM processing

2009-08-29 Thread Va Paula Sicurello
I have never heard of an EM lab making their own resin for embedding. We almost all buy the resin kits or the separate components to make either a Epon 812 equivalent or Spurrs. The amount of time it takes to process is usually tissue dependent. On average, it takes one day to process (if

Re: [Histonet] COX-2

2009-08-29 Thread shive003
In the meantime, you can google COX-2 aspirin colorectal and get caught up on the various articles written on the topic in the recent past. Jan Shivers UMN VDL On Aug 29 2009, Richard Cartun wrote: It was in USA Today, but will be published in this week's issue of JAMA. RWC Richard W.

RE: [Histonet] h.pylori silver staining

2009-08-29 Thread Weems, Joyce
And to that we add the Alcian blue, HE modification portion of the Genta stain procedure. Works perfectly. Joyce -Original Message- From: histonet-boun...@lists.utsouthwestern.edu on behalf of WILLIAM DESALVO Sent: Sat 8/29/2009 2:38 PM To: rjbu...@yahoo.com; histonet;