Hey, I thought you meant that famous sandwich ...Bacon Lettuce and Tomato -
guess it must be lunchtime
On Tue, Sep 29, 2009 at 3:20 PM, Kathleen Jones kjo...@upei.ca wrote:
Hello Histonet
I am wondering if anyone out there is familiar with the Barbeito-Lopez
Trichrome stain. I am using it
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Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent
Health is faithful to the healing ministry of Jesus Christ, providing high
quality care for the body, mind and spirit of every person.
The information contained in this
Hi Histonetters,
If anyone has a good protocol for staining plastic sections, could they share
it? We have samples embedded in an epoxy resin for TEM but they love the
semi-thick sections on the glass slide and asked if we could do something other
than a toluidine blue O, the standard stain
Hi Lesley:
There is a fairly large literature on this subject. I my opinion, most
of the fancier stains are not worth the effort and the results are
disappointing. In a separate mailing, I will send you some references.
Geoff
Lesley Bechtold wrote:
Hi Histonetters,
If anyone has a good
Hello all,
We're looking for a place that will do IHC for Pasturella multocida or where we
can purchase some antibody to do the test ourselves.
Anyone have any ideas?
Thanks
Denise Long Woodward, MS, HTL (ASCP) QIHC
University of Connecticut
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I use McNeal as a substitute for HE. I use it on MMA embedded bone.
It is to intense (for us) to be used on JB-4. I think you might need to
try a few different stains to select the best.
*/ /*
*/-- You are what you do - not what you say --/*
*/Karen Bowden
Staff Research Associate II
Our lab recently had our CLIA inspection and the surveyor informed me that we
need to discard any dry chemicals with an opened date of more than 10 years,
even though none of the dry chemicals have an expiration date. This was not
written up as a deficiency, but it was suggested we follow up on
Our dilemma has been that we aren't sure how they should be disposed of. We
have chemicals that are probably more than 30 years old.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
From: histonet-boun...@lists.utsouthwestern.edu on behalf of
My laboratory had many chemicals that were over 10 years old when the
facility started to prepare for ISO 17025 accreditation status four
years ago.
The facility's current policy for stock chemicals without expiration
dates is to set a 5-year expiration period from the date the substance
is
Hi Kelly and Lynn,
Can you not ask the surveyor for their reference research literature to
indicate that 10 years and older, the chemicals in your list would not be
good at all? And if you need to dispose of them, could the surveyor please
let you know how to dispose of it? If they want it
When we renovated our lab, we also had many chemicals to discard. We
used the same company that hauls our waste xylene away. The company we
used was Safety-Kleen, based in Saginaw, Michigan. They came in, made a
list of all the chemicals and then gave us a lovely price for
disposal/hauling
Under separate cover I sent an article for Stains for Plastic Embedded Tissue
Sections which originally appeared in Stain Technology 42:9-14 (1974) by
Humphrey and Pittman. I received it as an LKB applicaton note 303. The article
describes an HE stain for plastic sections, using methylene
HISTOTECH
Part Time Per Diem Position Available..
Mon-Fri 20-40 hours a week depending on amount of work and needs of the lab (
330am- 730am 30-80 blocks... Embed and cut Path Asst. will stain,
coverslip, QC and hand out the slides.
Call me if you would like more info. Please,
In response to the use of Pin 4 staining on various platforms, I have
used the PIN 4 from Diagnostic Biosystems on the Ventana Benchmark XT
and the results were fantastic!
Martha Brereton
860-442-0711
This message (and any included attachments) is from Lawrence Memorial
Corporation,
Allied Search Partners, is now accepting resumes for a qualified Histology
Supervisor in Corpus Christi, TX area.
To apply for this position please send resume to
aly...@alliedsearchpartners.com for intial prescreening of resume. Upon
receipt of resume one of our recruiters will hold a
What CLIA regulation did they use for reference for that statement?
Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220
From: histonet-boun...@lists.utsouthwestern.edu
I was going to comment about how important some people seem to feel to find
something to add to the inspection report even when it is unsubstantiated,
about how many of those chemicals are extracted from mines where they have
existed for eons, how the only important thing is to make sure that
I always have a problem determining the expiration date of sodium chloride!
Jim
___
James E. Staruk HT(ASCP)
www.masshistology.com
www.nehorselabs.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
The problem is letting people with little or no knowledge of chemistry make
chemical decisions. Some chemicals are unstable on the shelf, some are not.
Methyl green decomposes in a year or two, tetracycline decomposes in 5 years,
indigo lasts for millennia. Indigo used by Bar Kochba's
Can somebody share a Non specific esterase Enzyme hsitochemistry protocol that
works well with frozen muscle tissue. I have a protocol that I have used for
ages but it does not give me a very strong reddish brown staining on my
angular fibers. I will be happy to have it and compare. Thank you.
We have some Biocare Intellipath reagents that we are looking to get rid
of/donate. No charge but I can not pay to ship them. We are located in metro
Detroit. If anyone is interested please send me an email or call.
Thanks
Tony
bor...@trinity-health.org
248-858-6231
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