[Histonet] pkh

Hi all, We have collected a specific lung cell via FACS from mice, stained the cells with PKH, and then returned the cells to congenic mice via 1. tracheal injection or 2. tail vein injection. We can see the PKH positive cells in the recipient lungs on microscopy, but we are having a real

[Histonet] Fw: Pathos Tissue Processor vs Tissue Xpress

--- On Wed, 10/14/09, Rene J Buesa rjbu...@yahoo.com wrote: From: Rene J Buesa rjbu...@yahoo.com Subject: Pathos Tissue Processor vs Tissue Xpress To: Kiranjit Grewal kira...@sbcglobal.net Date: Wednesday, October 14, 2009, 9:28 AM Under separate cover I am sending you an article where

RE: [Histonet] lid gasket for processor

You might be able to shrink it a little by putting the gasket in the refrigerator for a while. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Diana McCaig Sent: Wednesday, October 14, 2009 6:34 AM To:

RE: [Histonet] Fw: Pathos Tissue Processor vs Tissue Xpress

Hi Donna: Thank you for your comment. As you write if the Pathos Classic is still available what I wrote is still valid. Unfortunately when somebody writes an article the conclusions remain valid as long as the subjects don't change, but the approach remains. As you can see I have forwarded

Re: [Histonet] Isotype background

I wouldn't get rid of the isotype control altogether; it's fulfilling it's purpose in trying to tell you something. Have you tried a no-primary control in parallel with your isotype control to see what the secondary is binding to - the tissue or bound goat IgG? What type of tissue is it? Maybe

[Histonet] HerCep

Does anyone have any experience using the Dako HerCep kit? And do you have any problems with cytoplasmic staining? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] unsubscribe, please

I wish to unsubscribe and return in 6 weeks. Thank you, Susan E. Bryant Knoxville Dermatopathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RELIA Histology Job Alert Histotechnologist needed in Atlanta

Hi Histonetters! I have a new position that I want to let everyone know about. I am assisting a client located in Atlanta GA that is in need of a histotechnologist. This is a full time permanent position on the night shift (12:30a-8:30a). My client offers excellent benefits and compensation and

Re: [Histonet] unsubscribe, please

Good for you! You should probably FOLLOW THE LINK IN EVERY EMAIL JUST LIKE WE SAY EVERY TIME SOMEONE WRITES IN TO UNSUBSCRIBE. Emily ...the thrill of being close to that hidden knowledge. That's the way I feel when I read Nabokov. Encrypted within his words, encoded indecipherably, ambiguously,

Re: [Histonet] HerCep

Depending on the tissue fixation processing, you can see some cytoplasmic staining. How does the cell pellet control look? We seldom see cytoplasmic staining on that. Are you tracking that your retrieval buffer is 95-100 after you add the slides starting your retrieval times when the buffer temp

Re: [Histonet] unsubscribe, please

LOL! Oh my I can't stop laughing...must be a serious, stressful day... --On Wednesday, October 14, 2009 12:56 PM -0400 Peter Carroll carro...@umdnj.edu wrote: FOLLOW THE LINK IN EVERY EMAIL JUST LIKE WE SAY EVERY TIME SOMEONE WRITES IN TO UNSUBSCRIBE Histonet Fun-Fact: Hits for the

RE: [Histonet] unsubscribe, please

Remember when we did do it that way and we had a count of how many ways you can spell it - unscribe, unsuscribe, etc! Ah the good ole days... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M

[Histonet] Bielschowsky/Luxol Fast Blue stain

Hi, Does anyone have a method for doing a combination of Bielschowsky Luxol Fast Blue? If anyone has the method any comments on it I would appreciate it. Thanks Sharon Allen HSC, Winnipeg, MB, CA sal...@hsc.mb.ca This email and/or any documents in this transmission is intended for the

[Histonet] Isotype background

Hi Adam, I always do the isotype control parallel with a no-primary control in parallel with my real Ab. May I suggest you to use Protein block serum free and pure Ab diluent without adding 2% donkey serum? Try this and let me know your results. If histonetters think I am wrong, fill free

[Histonet] Dayton Ohio Position Open

Hey All A fulltime Histotech position is open at Dayton Children's Hospital in Dayton Ohio. We are a small hospital. We have one part time, one full time (that could be you) and myself. We process about 5000 cases a year, we average about 15-40 blocks a day. Monday's are kinda heavy with

[Histonet] Validation of IHC

Our lab is inquiring about how other labs are validating their IHC stains. We currently are processing specimens both in a microwave and conventional processors. Are labs validating every type of program on the conventional and microwave processors? (i.e. small biopsies vs. larger tissue

[Histonet] Re: Isotype background

Hi Adam, We have had the exact same experience you have sometimes when we're using Goat isotype and Rabbit isotype negative controls. Sometimes you can fiddle with the protocol and minimize some of the background, and sometimes you can't. Rabbit and goat antibodies/Igs are notoriously sticky.

[Histonet] PHH3

Anyone in histo-land who has worked up PHH3 (anti-phospo-Histone H3, mitosis marker). I would like to perform this immuno on paraffin sections that are formalin fixed, using the Ventana Benchmark XT platform. My primary workups will be one brain tumors. Thanks for any help that you may be