[Histonet] OT - adoptive t cell therapy

Hi all, this is a very OT subject, but does anyone know about the above therapy in regards to adenocarcinoma? (i know that trialls are being done on melanomas presently). i would be grateful for any info -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South

[Histonet] Bone labelling for Microdamage

Is it ok to process cortical bone, embed in MMA, section and grind on the Exakt system. Last do the bone labelling stain? Or do I have to do the bone labelling stain prior to embedding, sectioning and mounting on slides? Any suggestions would be greatly appreciated. -- Karie L Reaser A.S. New

Re: [Histonet] Bone labelling for Microdamage

Karie, If you are looking for microdamage or are using basic fuchsin, I have been taught to bulk stain with 1% basic fuchsin in your processing solutions (i.e. 1% BF in 70% EtOH, 1% BF in 80% EtOH, 1% BF in 95% EtOH, and 1% BF in 100% EtOH) and then finish as you would with the remaining

Re: [Histonet] MMA Sections

Karen, How about coating your slides with Haupt's. I have used this solution exclusively for all my undemineralized thin section bone work. Also, I only need to dry my slides overnight using a dense aluminum slide press (available via Dorn and Hart Microedge). Basically, the slide press

[Histonet] tech schedules

I am posting this for my department manager. He is wondering how other labs schedule their techs. In other words, do the techs work fixed times (ie, come in always at 5 am) or do they rotate schedules (ie, come in at 5 am for one month, then 6 am the next month, etc). I will pass along your

[Histonet] Karie - microdamage in bone

Karie, Basic fuchsin works well for microcracks - stain before processing;cutting; grinding. Schedule used will depend in size of bone and species. You want to avoid over-staining. If time allows, check literature in: Journal of Orthopedic Research; Bone; and American Journal of Veterinary

Re: [Histonet] tech schedules

We do not rotate schedules here nor have we at any facility I've worked at over the years in this area.   Sheila Haas Laboratory Supervisor Micro Path Laboratories   From: Martha Ward mw...@wfubmc.edu To: histonet@lists.utsouthwestern.edu Sent: Tue, December

RE: [Histonet] SWOT Analysis

We have to do one for each new position or vacated position. I think that they are very helpful in justifying the needs of the department. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Scott, Allison D

[Histonet] large coverslips

Hello I'm looking for large glass coverslips, (about 1.5 inches square would be great, thickness #1 or 2). I've only been able to find 3 inch coverslips at brainresearchlab.com (from the histonet archive!). Has anyone seen any others out there? Emily ...the thrill of being close to that hidden

RE: [Histonet] Cryostat Poll

We have a LEICA CRYOSTAT... its EXCELLENT! Yes- we have a contract and NO never had issues... Maria Katleba HT(ASCP) MS Queen of the Valley Medical Center Napa CA 94558 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu