No, but you could substitute phosphotungstic acid. It's the metal salt
(tungsten, molybdenum) that's required for the stain, not the acid part.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
There has already been a lot of responses to this, but I thought I'd
add mine anyway.
For all our specimens in-house that are delivered from surgery, we
have three identifiers present: name, DOB and MR number. We
eliminated the use of SSN (from our reports as well), since we all
know how
Does anyone on the east coast have a Lica CM 3600 Cryomarocut cryostat? Need
to see one in operation.
Thanks.
Terry
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It is not because it is an acid. Phosphomolybdic acid has properties that
hydrochloric acid don't have. Short: NO
René J.
--- On Wed, 12/2/09, dcoj...@tampabay.rr.com dcoj...@tampabay.rr.com wrote:
From: dcoj...@tampabay.rr.com dcoj...@tampabay.rr.com
Subject: [Histonet] masson trichrome -
OK, light microscopy.
I think frozen sections of formalin fixed (formalin with calcium)
material is the way to go here. Wash out the formalin as it will react
with the dicrhomate then fix with Os or Os-dichromate.
Altman's fix is equal parts of 2% aqueous osmium and 5% aqueous
potassium
Debbie Boyd in Virginia asks about slide crushers:
At the National Society meeting in Birmingham recently, a company called
Creative Waste Solutions displayed a slide crusher. It grinds them up and the
slide labels too until it is just like very roughly ground sugar. The
instrument costs
Good Morning,
I am sure this question has popped up before, but, when you are running
H.pylori by immunohistochemistry do you have to run a patient pos and
patient neg for each patient. If you have 10 patients do the run consist
of 20 slides each patient having a pos and neg. What does the
Here we run a pos control which is on each patient slide and one Neg
control for every block such as A, B, Etc I believe this is the CAP
recommendation
You can look at all the specific guidelines by going to the CAP website
and looking at questions ANP.22550 and ANP.22570
Hope this helps
Hi All,
I know this might be a dumb question.
Has anyone been able to perform CO-IF staining with two rabbit antibodies with
confidence in specificity of the signals? I am worried that using the same
specie antibody with cause specificity issues. I'm interested in staining
p-S6RP and p-44/42
Regardless of what others may be experiencing with a similar station, I think
that you should check the filters of yours and the air circulation.
As to formalin substitutes I am sending you under separate cover an article on
the subject.
René J.
--- On Thu, 12/3/09, Kathy M. Gorham
I have just purchased two Mopec MB600 grossing stations and we do not have this
problem. Our pathologist actually rave about the unit.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham
Formaldehyde fumes require filters which have been treated with Potassium
Permanganate. Normal activated charcoal filter will not trap formaldehyde
fumes.
--
Best regards!
Cliff Berger
Decal Chemical Corp
1-800-428-5856 - Office
914-588-5019 - Cell
On 12/3/09 12:35 PM, Kathy M. Gorham
Our lab tried the self contained grossing station with filters
(bench-top) and we had troubles with it adequately filtering formalin
fumes (even though the spec sheet stated that was what it was for...from
MOPEC). We ended up installing a fume hood to use for gross prosection,
waste discard, etc.
I suggest having your bio-med or engineering department check the
generator exhaust belts that power the ventilation to see if they have
worn, slipped or have broken...
R.Brian Fischer
Histology Lead Tech
Community Hospital of the Monterey Peninsula
PO Box HH Monterey Ca. 93942
831-625-4791
Hi Jeri,
He should be fine. Passing the HTL demonstrates considerable knowledge
which is applicable to the clinical (human) lab world. Also, I'm of the
opinion that animal tissue - in general - is more difficult to section
than human tissue.
Not actually knowing the person - which makes a
His skill set should be fine for any lab setting. I have worked in hospital
labs and animal only private labs and had no problems.
Christie
From: j...@opssearchgroup.com
To: histonet@lists.utsouthwestern.edu
Date: Thu, 3 Dec 2009 14:48:31 -0500
Subject: [Histonet] (no subject)
Can someone help me with a protocol for making citrate buffer (pH 6.0)
for IHC antigen retrieval?
Justin Peters, HTL (ASCP)
IHC Supervisor
Bostwick Laboratories(tm)
For Absolute Confidence(r)
4355 Innslake Drive
Glen Allen, Virginia 23060
Phone:(804) 967-9225 ext. 1831
Cell:
http://www.ihcworld.com/_protocols/epitope_retrieval/citrate_buffer.htm
Jan Shivers
- Original Message -
From: Justin Peters jpet...@bostwicklaboratories.com
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, December 03, 2009 3:36 PM
Subject: [Histonet] buffer protocols
Can
We have also experienced an increase in formalin vapor levels since the
installation of our Thermo Grossing Station. It is self-contained and utilizes
potassium permanganate filters for formalin neutralization. Has anyone had any
quality control issues with these filters? Our last set blew
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