Hi all,
I am going crazy - and its not even friday yet! A little while ago (OK about
3 years) i came across a paper where the researcher had shown the transition
of macrophages to osteoclasts using immuno staining - i cannot seem to find
it on Pubmed, dont know the author date or journal.
i hope
A pH meter must be calibrated hourly or daily, not monthly!
In a research environment, with frequently changing chemicals that might damage
the glass electrode, you need to calibrate against standard (usually bought)
buffers several times within every day. pH meter electrodes are horribly
fra
Rene makes an excellent point as to the reason for having a license. I
agree that it is a good idea. Unfortunately NYS took the idea and botched
it. The NSH tried to push them in the direction of using the HT exam as
their qualifying exam, but instead they want to write their own and they are
pl
Hello
I have the same experience.
When I worked for a company and right after 9/11, we shipped antibodies to
Israel via Post office at ambient temp.
This package came back after 6-8 weeks or may be longer.
We do not know how they were stored at post office.
We tested the antibodies and could not fi
Hi,
We have a microm 505 E cryostat in the lab, the microtome part of which
a repairer came to clean. Since the return of the microtome to the
cryostat, when we turn it on we no longer get any of the error messages
specified in the manual (such as those that come up when the cryostat
has been off
Sorry I made a type "O" I meant to say Also, manufacturer's will
revalidate antibodies when they are close to their expiration date
and if that antibody has the same sensitivity. They will extend the
shelf-life date.
Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele:
I agree with Tim. This is a very common practice for antibody
manufacturer's. Also, manufacturer's will revalidate antibodies
when they are close to their exploration date and if that antibody
has the same sensitivity. They will extend the shelf-life date.
Akemi Allison BS, HT (ASCP)
Has anyone had any experience with this antibody? There are many
companies selling them and I'm not sure which would be best for my
paraffin-embedded human tissue?
Katherine Walters
Histology Director
Central Microscopy Research Facilities
85 Eckstein Medical Research Building
University of Iowa
Kim,
Test a random sampling (10%) of the antibodies and see if they work. If so,
then they are most likely fine. Document the test. Antibodies are very robust
and the warmup probably won't damage many, if any, of them.
BTW, When I worked for an antibody manufacturer I did a test once as part o
Marie,
If the person is licensed as a technologist with no ASCP certification
but at least 5 years of experience, he/she qualifies under rule
64B3-5.002(C)(3). See below.
64B3-5.002 Supervisor.
Qualifications and Responsibilities.
(1) Qualification. Degrees or semester hours of academic credit r
There doesn't appear to be anything wrong with the staining schedule.
Mayer published more than one alum hematoxylin and one of them is a strong
regressive type, and would produce the staining you see. Is it possible
that has been used instead of his progressive one? If it has, make the
foll
Hello Valerie,
I believe you may have been given misinformation regarding the knife
sharpening abrasives. You can order through Leica Microsystems using
catalog number 14041819700 for a single bottle or 14937000 for a case of 6
bottles.
Please feel free to contact me if you have any questions.
From: Hannen, Valerie
Sent: Friday, December 04, 2009 9:43 AM
To: 'histo...@list.utsouthwestern.edu'
Subject: Coarse abrasive
Help!! My section chief just got word that Leica is no longer producing
coarse abrasive. We only have steel blades in our department
Cell Marque , has a concentrated and a Ready to use. They both work great.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
You have to consider that the thicker the section the more intense the staining
will be.
If you are using now 1 minute and they are still too dark, reduce the staining
time even further.
Otherwise I don't see anything wrong with the rest of the protocol.
René J.
--- On Mon, 12/7/09, Josephine Ga
Anyone out there know were Glypican 3 antibody for IHC may be purchased from.
Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 719-365-8357
Ph: 719-365-6926
Fax: 719-365-6373
rick.garnh...@memorialhealthsystem.com
Mission
My teacher, long retired Miss Mavis Marie, a MT that loved Histology, taught us
that if we needed to do the staining the next day, to leave them in 70%
alcohol. We did find that sometimes, not always, if left in distilled H2O
overnight, it would wash off, but never did when left in 70%.
Hope t
The Moral here is, -don't just automatically toss your perceived mistakes, look
at them and see what happened!
Janet
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa
Sent: Sun 12/6/2009 11:50 AM
To: gu.l...@gmx.at; 'Robert Richmond'; P
Hi all,
My (frozen-section, fixed) slides are coming out much too dark (overstained
purple) and I'm not sure why. They are 15-20 micrometer slices of rat
gastrocnemius muscle. Can someone please look over our current protocol and
tell me what I'm doing wrong? Thanks! Here it is:
1. Perfuse animal
I will be out of the office starting 12/07/2009 and will not return until
12/08/2009.
__
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email
Seriously... you wouldn't drink milk that had sat in a warm fridge all
weekend... but you use reagents and antibodies that had?
Greg Dobbin wrote:
I agree with Renee. It will proabaly be at least as costly to have to
re-validate everything!
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic
One of the researchers here summed it up nicely when he reported that most
discoveries in the lab are not heralded with cries of "Eureka!", but rather
with "hmmmnow that's interesting..."
Teri Johnson, HT(ASCP)QIHC
Stowers Institute for Medical Research
Kansas City, MO
I agree with Renee. It will proabaly be at least as costly to have to
re-validate everything!
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PEC1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
Perhaps yes or perhaps no, that is the problem.
>From now on you cannot know if something that did not work was because of the
>fridge malfunction.
Advise? (Costly advise?): discard everything and get an alarm system for your
fridge after you repair it (or get a new one)..
René J.
--- On Mon, 12
Amazing! How hot do you keep the room temp there?
I would toss them, one false test on a patient is more valuable than all
those lost reagents.
Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996
Kim Merriam
Se
Looking for someone close to Pittsburgh, PA (or within a 300 miles) with a
Leica CM3600 that I could come by and look at.
Thanks,
Terry
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Help!
My refrigerator died over the weekend. This fridge had most of my antibodies
and IHC reagents. When I came in, the temperature inside the fridge was a
balmy 37C!
Do you think any of my reagents are still usable?
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
_
No, it is not a good idea. In many occasions leaving the dewaxed sections in DW
for prolonged periods of time can cause the section to peel off (not always,
but sometimes).
The idea is to dewax and do the special stains immediately after.
René J.
--- On Mon, 12/7/09, Charles, Roger wrote:
Fro
Cindy:
And not being able to hire anybody without the license is absolutely great and
why the license was created in the firs place, namely, to avoid having low paid
people doing histology work in detriment to others more qualified.
The salary competition posed by those not licensed is the cause
I wouldn't make that a habit. It eventually saturates the cells and then
they fall off like the previous poster mentioned. Also, especially leaving
them in water for a whole weekend you risk contamination. Not good
practice. If you absolutely have to leave them in something after they
have been
What would be the point??
Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Charles, Roger
Sent: Monday, De
I don't see any point in leaving deparaffinized and rehydrated(!) slides
any longer in water. The tissue might even fall off.
What special stains are you goint to do?
Hello all,
I need some information on special stains in regards to handling of slides.
Would it be good practice to deparaf
Hello all,
I need some information on special stains in regards to handling of slides.
Would it be good practice to deparaffinize and rehydrate slides then allow them
to sit in water over night or over the weekend before doing the actual staining?
Thanks
Roger
Roger Charles
Microbiologist
Penn
Tom,
I agree with you about Nate qualifications. Unfortunately in NYS if you
bring in outside work your tech MUST be licensed in NY. The copy of the
license need to be displayed in the lab. I haven't found any way to hire
employees without a license.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
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