Re: [Histonet] fish scales

2009-12-12 Thread John Kiernan
Fish scales are calcified. Depending on the type of fish, they may contain dentin, bone or both. My sources: Two zoology textbooks and also a Wikipedia entry, Scales (zoology) that reads easily. Even boneless fishes like sharks have calcified teeth and skin. John Kiernan Anatomy, UWO London, C

Re: [Histonet] basic IHC guide

2009-12-12 Thread John Kiernan
Dear Anthony Sandoval, It's a breath of fresh air to see someone using Histonet to ask about a book rather than ask for a "protocol". For simple but thorough explanations and easily followed instructions I strongly recommend Polak, J.M., and Van Noorden, S. (1997) Royal Microscopical Society M

Re: [Histonet] basic IHC guide

2009-12-12 Thread Rene J Buesa
Contact DAKO because they have a IHC guide available that is pretty good. René J, --- On Sat, 12/12/09, Anthony Sandoval wrote: From: Anthony Sandoval Subject: [Histonet] basic IHC guide To: "histo net" Date: Saturday, December 12, 2009, 4:00 PM Hello Histonet, I am pretty new to IHC and w

RE: [Histonet] basic IHC guide

2009-12-12 Thread Liz Chlipala
Get the book from Dako - Its called Immunohistochemical Staining Methods - 4th edition. It's a great reference and its free. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.

[Histonet] basic IHC guide

2009-12-12 Thread Anthony Sandoval
Hello Histonet, I am pretty new to IHC and would like to study a basic text book. Can anyone give me a recommendation? Thank you for the support! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/li

[Histonet] Using acetic acid alone as a fixative

2009-12-12 Thread gayle callis
Dear Histonetters, I have a person using acetic acid as a fixative for frozen sections containing bioluminescent proteins (GFP and tdTomato, a red fluorescent protein). I have always been taught to use acetic acid in combination with other chemical agents (e.g. as in Bouins or Carnoys) b

[Histonet] Re: Help with Large Eye Specimen

2009-12-12 Thread Robert Richmond
Sally Breeden, HT(ASCP) at the NM Dept. of Agriculture Veterinary Diagnostic Services in Albuquerque asks: >>I do not have the equipment to do a whole horse eye (with levels) on >>"lantern" slides. We rarely have call for this and my boss would like to know >>if there is a vet school or referenc