Hi All,
Has anyone ever used this antibody in FFPE mouse or rat tissue? How
easy of an antibody is it to work with? Other comments? We may be
doing this for an upcoming project and I just wanted to know what I
could be getting myself into. Thanks!
Jen Campbell
Does anyone know of a company that repairs equipment in the Maryland area? I'm
in Bethesda and my old Tissue-Tek embedding center won't hold the temperature.
Thanks for any help,
Ruth Yaskovich
National Institutes of Health
Bethesda, Maryland
___
Is there a procedure or some standard method validation process out there
for starting up a new tissue processor? Maybe one that would be approved
by regulatory agency?
I am not sure whether this would just be left up to the pathologists to
decide what they would prefer.
Thank you,
Carole
This question is for those of you who perform fine needle aspirations.
What stain are you using for your immediate evaluation? Or do you give an
immediate evaluation/adequacy?
Thanks.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park
Hi, We use Tech One Biomedical Services, Inc. as a third party PM and
repair service. They are very nice to work with and not excessive with
their fees. We have set up PM's with them for a lot of our older
equipment. Toll free number is 866-497-3033 or www.techoneweb.com . Try
them for your
Hi Everyone,
I have yet another IHC-related question. So after antigen retrieval,
and then allowing my slides too cool, I place them in wash buffer before
loading them on to the autostainer. Would leaving the slides in the
wash buffer in the fridge for an extended period of time (say, 1
I guess yes.
Unless I can say exactly which ingredient will affect my staining, I
would not leave them in any solution for such a long time!
Hi Everyone,
I have yet another IHC-related question. So after antigen retrieval,
and then allowing my slides too cool, I place them in wash
Hello Histonetters,
I ran into an issue when staining some TMA slides the other day. This question
isn't about how to fix the staining, or lack there of. I would like to know
whether or not it is possible to repeat the same protocol on the same slides
after removing the coverslips. If any of
Hi, I am posting this question for Lisa,
Our Microbiology department would like to find a good calcafluor
staining method and a good Auramine/Rhodamine staining method for FFPE
tissues. Any suggestions? Would you be able to post this for me?
Thanks, Lisa
Lisa Manning MLT, BSc.
Pathology
Does anyone have VZV Controls? A block would be great, but at this point I will
take anything. Can anyone point me in the right direction in finding this?
Thanks
_
Your E-mail and More
OK Guys, here's another JCAHO/CLIA controversy. Has anyone (Mostly MOHS labs)
been sited for this? Currently we only save tissue specimens 24 hours in case
the Dr. decides to send some to path after the fact. (don't get me going on
morphology quality). Does anyone have a clear definition of
I'd highly recommend Randy Stephens.
randysteph...@mac.com
Jan Mahoney, Omaha, NE
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perkocha, Luke
Sent: Wednesday, December 16, 2009 12:57 PM
To:
I would never do that. Why subject your sections to such a treatment?
René J.
From: Jennifer Campbell jcampb...@vdxpathology.com
To: histonet@lists.utsouthwestern.edu
Sent: Wed, December 16, 2009 1:34:40 PM
Subject: [Histonet] leaving IHC slides in wash buffer
Dear Histonetters,
I am getting background staining with my Immunohistochemistry protocols that
require CC1 and CC2. I am thinking of adding AB block to the protocols. Do
you have any other suggestions?. I use the ULTRA stainers.
Thank you very much in advance,
Milton A. Gomez, HTL
Hi Guys!
I've used a potassium hydroxide solution to 'soften' nails after fixation but
before processing. I cannot find my procedure!!
Can anyone help?
Cheryl Kerry, HT(ASCP)
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
We use the Diff quik (or whatever spelling you use!)stain on air-dried
smears.
There is also a rapid Poor man's HE stain, using the Diff Quik
solutions, that you can also use. You can then restain with the normal
PAP stain back in the lab (Hirschowitz et al Acta Cytolog (1994)
38:499-501)
We try
These are brand new installs. it does it only for the CC1 and CC2 Protocols.
Milton A. Gomez, HTL (ASCP)
Technical Supervisor
Immunohistochemistry Department
ARUP Laboratories, Inc.
500 Chipeta Way
Salt Lake City, UT 84108-1221
Desk Phone: 801-583-2787, ext.3869
Lab. Phone: 801-584-5257/5242
Hi-
I am doing immunohistochemistry on fresh frozen tissue using the
capillary gap method on Tecans. The preferred fixative for my antigen
is a cold acetone fix. I am using MeOH/H2O2 to kill peroxidase before
the blocking step and it's resulting in many small bubbles forming on
the tissue
you can use the same slides , repeating all the steps except the antigen
retrieval ( if it is the same ) Ideally you should use new slides, but on tiny
bxs, outside slides, or very limited area of interest, that is not aways
possible.
Rena Fail
- Original Message
From: Thomas Pier
we use 20% sodium hydroxide. Works great
- Original Message -
From: Cheryl tkngfl...@yahoo.com
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, December 16, 2009 4:08 PM
Subject: [Histonet] looking for KOH nail procedure
Hi Guys!
I've used a potassium hydroxide solution to
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