[Histonet] Wright-Giemsa stain

2010-01-22 Thread Marshall, Kimberly
I am from a small Hospital where due to the size, Histology does the Bone Marrow's. We go to the procedure, make the smears, as well as do the Wright-Giemsa stain. I am having a hard time getting the Wright's stain dark enough for my Pathologist. I have added stain time, checked the pH of my s

[Histonet] IHC stain for TFEB

2010-01-22 Thread Justin Peters
Does anyone know any reference labs that would be able to perform an IHC stain for TFEB? Justin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Histology Manager job opportunity

2010-01-22 Thread Melissa Ribeiro
My client, a 300-bed hospital in Southeastern Connecticut has an immediate opening for a HISTOLOGY MANAGER. The Histology Manager is responsible for managing and supervising 2 full-time Histology staff members, and ensuring that the department runs effectively. Additional responsibilities in

RE: [Histonet] Joel - re: tendons

2010-01-22 Thread Liz Chlipala
Vicki is correct for larger samples I process manually with graded alcohols - overnight for each step till I get to the last 100% alcohol, I do 3 100% for larger samples (we process large 2 x 3 tissue both soft tissue and bone routinely). You might think that these tissues will be over processed b

[Histonet] re;Biomedical repair

2010-01-22 Thread Cheri Miller
Thanks to all of you who responded. I had no idea I would catch so many peoples attention, especially Vendors (yikes). I will think twice before I send another one for sure. I have heard good things about Hawkeye Biomedical from many of you and they are currently working with me on getting what

[Histonet] Joel - re: tendons

2010-01-22 Thread Vicki Kalscheur
Joel: Increase all stations of processing time, as Liz suggested. For large sections of tendon, or when looking at length with both tendon attachments, I process by hand and soak in paraffin changes over-night (oven). Unless you have a bone attachment, decalcification should be unnecessary. Te

Re: [Histonet] FISH Qualifications/Training

2010-01-22 Thread Rene J Buesa
Usually counting is done by a trained histotechnologist. Some companies, like Abbott, have training/certification programs. A pathologist always has to interprete the results, and sign the case. René J.     --- On Fri, 1/22/10, Remy Lopez wrote: From: Remy Lopez Subject: [Histonet] FISH Qual

[Histonet] FISH Qualifications/Training

2010-01-22 Thread Remy Lopez
Good Morning! Does anyone know what are the requirements or qualifications for people to be able to read HER 2 FISH slides? Does it have to be a pathologist? Are there any courses/training available for this? Thanks Remy ___ Histonet maili

Re: [Histonet] tamponated formaldehyde

2010-01-22 Thread Rene J Buesa
Sue: In Spanish, French and Italian a buffer solution is called a "tampon" solution. So "tamponated", which is a word nonexistent in English, "wants" to mean a "buffered" solution. René J. --- On Thu, 1/21/10, Sue Reilly wrote: From: Sue Reilly Subject: [Histonet] tamponated formaldehyde To

[Histonet] Reference lab for "Pro Ex C"

2010-01-22 Thread Sebree Linda A
Hello histonet, We are in immediate need of a reference lab that performs Pro Ex C on human FFPE tissue. Thank you, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 __

[Histonet] xylene alternatives

2010-01-22 Thread MaryAnn Dixon
Hi Histonetters, I would like to start using a xylene alternative in my stain line but are unfamiliar with the latest products. I've heard of slidebrite and propar only. All opinions and suggestions are greatly appreciated. Thanks! MaryAnn MaryAnn Dixon BS, HT (ASCP)cm Biological Scientist Surg