[Histonet] fontana

Does anyone know of anything other than melanin that Fontana Masson would stain? Also, does anyone know of a stain that would label un/saturated fatty acids? Not sure if this is even possible. Thanks in advance. Kris Kalleberg ___ Histonet mailing

[Histonet] Travel positions?

Does anyone know of any travel positions? I have traveled in the past for a couple of years and I have 7 years experience all together. I am willing to go anywhere. Thanks Leah Cox ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Bcl-2

Hi everyone, I am hoping you all can help me solve a problem that I am having with my Bcl-2 stain. I can get it to stain Follicular Lymphoma tissues, but for the life of me can not get normal tonsil tissue to stain. I use a Retrieval solution with a pH of 8, use Avidin / Biotin solutions,

Re: [Histonet] Double labeling with antibodies that need different fixatives

I haven't tried it directly but I don't see why not. If it's an amplification problem you can always amplify the fluorescence. That being said I am really not a huge fan of IF on FFPE especially when it comes to vessels as many vessels have high autofluorescence not to mention the

[Histonet] Tris Buffer Preservative

Hi, I have been making up my own tris buffer for immunohistochemistry. It has been working swimmingly, with one exception. I have been doing some work on Salmonella, and apparently the researcher has been seeing bacteria in her immunofluorescent slides that she says are moving and seem to be

RE: [Histonet] fontana

Fontana-Masson also stains for Argentaffin granuals. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

Re: [Histonet] fontana

Any histological structure able to reduce silver into a black precipitate (argentaffin reaction) will be stained with the Fontana-Masson procedure. René J. --- On Wed, 2/24/10, Kalleberg, Kristopher kristopher.kalleb...@unilever.com wrote: From: Kalleberg, Kristopher

Re: [Histonet] Bcl-2

I always did HIER at pH6 for BCL-2, diluted  at 1:10 (DAKO Ab) during 30 min incubarion. FFPE tonsil as control. René J. --- On Wed, 2/24/10, Hannen, Valerie valerie.han...@parrishmed.com wrote: From: Hannen, Valerie valerie.han...@parrishmed.com Subject: [Histonet] Bcl-2 To:

Re: [Histonet] Tris Buffer Preservative

I never used preservatives but I also never stored the buffer more than 5 days. René J. --- On Wed, 2/24/10, Amos Brooks amosbro...@gmail.com wrote: From: Amos Brooks amosbro...@gmail.com Subject: [Histonet] Tris Buffer Preservative To: histonet@lists.utsouthwestern.edu

[Histonet] Sirius Red

Is there a good Sirius Red procedure used to stain collagen And where can I find the powder ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Sirius Red

Thanks for everyone's help From: John Kiernan [mailto:jkier...@uwo.ca] Sent: Wednesday, February 24, 2010 11:36 AM To: Nails, Felton Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Sirius Red You'll find brief instructions for the picro-sirius red

Re: [Histonet] Sirius Red

I use the Puchtler's Picro-Sirius Red Stain that I got from Stains File online. This stain works beautifully and the collagen is really pretty when using polorized light microscopy. The Sirius Red that I use was here in the lab so I didn't have to buy it but it is Sirius Red F3B. Andi

AW: [Histonet] fontana

Kris, Formalin pigment would give false positive staining with Fontana Masson. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Cynthia Pyse Gesendet: Mittwoch, 24. Februar 2010 18:05 An:

[Histonet] M.O.M frozen section background

Hi All, Does anyone have any tips on reducing background staining when attempting to detect mouse monoclonal antibodies on mouse tissue? We currently use Vector's M.O.M kit - it works great on PPFE tissue with nice specific staining after pepsin unmasking, but in frozen tissue the background

RE: [Histonet] Paraffin Accumulation on floor

We have a long-handled scraper and use it periodically. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

RE: [Histonet] Paraffin Accumulation on floor

We also use the long-handled scraper. Our greatest problem though, was the hallway outside of Histology where non-technical staff would be walking in heels. We eventually had that hall carpeted, which has been a great improvement. -Original Message- From:

RE: [Histonet] Paraffin Accumulation on floor

We just bought everyone skates. Seriously, we have a scraper that we use, along with the various mats. What we just did was lay the traction strips on the floor outside of the Histology lab. That was the area were most people, myself included have slipped and fallen. Ironically, we found no

Re: [Histonet] Tris Buffer Preservative

Hello, Amos, It is pretty straightforward to make fresh, why would you want to store it? We use sterile 1M Tris, pH 7.4, and 4M NaCl as stocks, they are concentrated enough that nothing obvious grows in them, dilute the volume you need, pH if needed, (add the Tween if needed), and store in

[Histonet] disinfectant for ventana xt decon process

Hi All, I am trying to get AMPHYL disinfectant for the decon procedure for ventana XT but heard that the product is discontnued by the manufacturers. If anyone is using a substitute for this, please give me the vendor and the part number. Thanks Nirmala Srishan Holy Name Hospital is

[Histonet] FISH PROBES

Does anyone know of a reliable company to purchase FISH probes from (other than Abbott)? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] FISH PROBES

IDLabs http://www.idlabs.com/home/ works great for us. Cambio is referenced a lot in the literature, but their Y-chromosome porcine probes didn't work for us. http://www.cambio.co.uk/home --On Wednesday, February 24, 2010 2:42 PM -0500 dcoj...@tampabay.rr.com wrote: Does anyone know of

Re: [Histonet] M.O.M frozen section background

What types of mouse tissue are you working with? Are they fresh frozen with post-fixation or fixed frozen? Do you block biotin? Do you have an isotype control and a no primary control?  I am thinking biotin's the likely culprit as the kit doesn't come with the very necessary biotin blocking

Re: Re: [Histonet] Double labeling with antibodies that need differentfixatives

Not sure if you were asking me or not and it's a broad answer but VE-cadherin is a good one - the best overall marker of endothelium. vWF is another one that works well for most tumors. If staining mouse organs, if you let me know what tissue you are staining I can recommend the best specific

[Histonet] job

Looking for part-time histotech Private lab in Plano Tx. 972 981 3108 The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from

[Histonet] Snap Freezing Tissue

I was wondering how others are snap freezing tissue? We have been using a histobath (a refrigerator device that cools isopentane for freezing tissue), but it has died. I would love to have another Histobath, but they are no longer carried by the vendor that I originally bought mine from. Does

Re: [Histonet] Snap Freezing Tissue

I have used the Gayle Callis method of freezing tissue for histological applications for years with great success :) The tissues look fantastic.   Essentially float out a metal pan (like the ones you sterilize surgical tools in) on a liquid nitrogen bath (in a styrofoam container or large

Re: [Histonet] Snap Freezing Tissue

Contact Jim Mullen at Hacker Instruments (800) 442-2537. He has a demo CliniRF that I'm sure you can get at a good price. Dorothy Dorothy Traczyk MTA Histology LLC In a message dated 2/24/2010 4:55:55 P.M. Eastern Standard Time, laurie.colb...@huntingtonhospital.com writes: I was

RE: [Histonet] M.O.M frozen section background

You made me worried there for a minute, Charles - I've spent a lot of time cryosectioning rather than using PPFE! But luckily dehydrating, rehydrating and post-fix seemed to completely get rid of the background I was experiencing. No idea why it works, but it did. (We cryoprotect In sucrose, then

[Histonet] Decalcification and processing of large bone

Dear all,   I am going to decal and process PIG femur and lumbar vertebrates.  I only have experience on soft tissues.  Anyone can suggest any protocols in decalcification and processing?  Many thanks in advance.   Cheers, Victor ___ Histonet

Re: [Histonet] Paraffin Accumulation on floor

I have noticed that most slips happen in the junctions between the paraffin covered floors and uncoated floors, such as doorways to outside halls. The change in traction does it. Also, hard soled shoes are fine, as long as they have a build up of paraffin on them. If a visitor (or