I use the Puchtler's Picro-Sirius Red Stain that I got from Stains File online. This stain works beautifully and the collagen is really pretty when using polorized light microscopy.

The Sirius Red that I use was here in the lab so I didn't have to buy it but it is Sirius Red F3B.

Andi



Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

[email protected]
Tel: 520.626.4415     Fax: 520.626.2097

"happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello
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On Feb 24, 2010, at 10:22 AM, Nails, Felton wrote:

Is there a good Sirius Red procedure used to stain collagen
And where can I find the powder
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