Are you sure that you don't introduce air bubbles when you put your slides into
the coverplates? The antibody will not touch the tissue if there is an air
bubble.
Willem Hoekert
Van: histonet-boun...@lists.utsouthwestern.edu namens Rene J Buesa
Verzonden: do
Dear Histonet Friends,
I just wanted to share an incident we recently had with an old paraffin pot.
One of my techs came in on Sunday to embed some tissues, went into the
processor room and smelled something burning. He noticed our old paraffin pot
had charred looking labels on the outside
Not exactly the same, but very similar. We had an automatic stainer by
the sink and one of the techs was washing glassware, the stainer was
running. The water apparently splashed on the wiring and a fire broke
out. We jumped into action. Just as we had been in-service. You are
correct
Hello,
I'm glad that you already solved the problem your way.
I didn't read your first post, but we had exactly the same problem (and
we're also using cover plates). This artifact is very likely caused by tiny
air bubbles which are trapped under the cover plate. The crucial step is
when you drip
Hello histotechs- please email me if you're interested in working a few
hours on Saturday mornings. We are flexible, and have another Saturday tech
that can fill in if you can not work on certain dates. I'll give you more
info privately. I'm only seeking histotechs who have at least 2 years
A few years back, I also had a fire break out while cleaning off the weighing
instrument. There was left over powders of other chemicals on there and my
gauze started on fire. So needless to say, please clean up after yourselves! It
was Scary, very scary! I tried to yell for help but
I did the same thing several years ago - caught my uniform on fire but
ran to the sink and all was well in a minute - techs ran to my rescue.
But then one of the gang said... Grab the marshmallows, the
supervisor's on fire!... Gotta love em...
Happy Friday!! J
Joyce Weems
Pathology Manager
Hello friends
I have some gill tissues for chloride cells localization.
Do you know a method for chloride cells coloration except
immunohistochemical using NKA antibody???
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
One of those chemicals could have been chromic acid which will ignite when
alcohol is introduced. One of my techs cleaned up the counter with alcohol
after measuring chromic acid and we all ran for the extinguisher. She just
stood there and shook her hands and stared at the flames. This was
Why you just don't Google it?
René J.
--- On Fri, 2/26/10, Yaskovich, Ruth A (NIH/NIDCR) [E]
ryaskov...@dir.nidcr.nih.gov wrote:
From: Yaskovich, Ruth A (NIH/NIDCR) [E] ryaskov...@dir.nidcr.nih.gov
Subject: [Histonet] Crystal/Mount
To: histonet@lists.utsouthwestern.edu
Does anyone have an SOP for monitoring room temp and humidity they can share
with me?
Thank you,
Ann
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Is there a minimum time for prostate biopsies to be fixed. Does
fixation times affect PIN4's?
Diana
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I work in a small research lab, and we are shopping for a new tissue embedding
machine. Our current unit is a very old AutoTechnicon carousel style system.
Space is limited, and we do not have large numbers or constant flow of samples
to embed, so we are happy to stay with the carousel
Thanks for catching my typo..., we are looking for a carousel style tissue
processor.
Cathy
From: Richard Yeo [mailto:r...@wchosp.org]
Sent: Friday, February 26, 2010 4:04 PM
To: Parsons, Catherine
Subject: RE: [Histonet] Replacing an AutoTechnicon - reviews on modern carousel
tissue embedding
A poor fixation will affect all the cell components. You may have a result but
if the fixation is incomplete, the results will not indicate the real amount.
At least 8 hours of fixation will be required.
René J.
--- On Fri, 2/26/10, Diana McCaig dmcc...@ckha.on.ca wrote:
From: Diana McCaig
The Leica TP1020 is a good and reliable instrument.
René J.
--- On Fri, 2/26/10, Parsons, Catherine cpars...@vt.edu wrote:
From: Parsons, Catherine cpars...@vt.edu
Subject: [Histonet] Replacing an AutoTechnicon - reviews on modern carousel
tissue embedding machines
To:
Thermo Fisher or whatever they are called this week has it
Joe
- Original Message -
From: Rene J Buesa rjbu...@yahoo.com
To: histonet@lists.utsouthwestern.edu; Ruth A (NIH/NIDCR) [E]Yaskovich
ryaskov...@dir.nidcr.nih.gov
Sent: Friday, February 26, 2010 2:28 PM
Subject: Re: [Histonet]
Once upon a time in a far away land, we used to boil our embedding molds in
boiling soapy water, over an open Bunsen burner, followed by an alcohol
rinse then air dry. One time the fire alarm was activated and we had to
evacuate the hospital. We were out there quit awhile. When we received the
You're quite right René .
Googling crystalmount brings up a helpful item (for a competing mounting
medium) at the top of the heap, with detailed instructions for use.
The third and fifth hits are also helpful. Unfortunately these are followed by
hundreds of items, most having nothing to do
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