[Histonet] Need help with FISH staining protocol on frozen tissue sections

Hi everyone, Has anyone done FISH staining on mice tissue frozen sections for Y chromosome? I need help with a successful protocol. Recently I started to do FISH staining on mice liver frozen sections for chromosome Y, but got no positive results even on male samples yet. The probe I used was

[Histonet] Masson Trichrome, Alligator Heart Tissue, colors turning out purple

To Histonet: This is my first time mailing the histonet community. Coincidentally, this is my first venture into histology. My lab is conducting research on alligator heart tissue. At the moment, I am attempting to stain my slides using Masson Trichrome to differentiate muscle from connective tiss

Re: [Histonet] Butyl alcohol

As an additional note: paraffin oil = mineral oil. René J. --- On Tue, 3/9/10, Michael Folsom wrote: From: Michael Folsom Subject: Re: [Histonet] Butyl alcohol To: "Krueger, Todd" Cc: histonet@lists.utsouthwestern.edu Date: Tuesday, March 9, 2010, 11:17 AM Hi: I'm not sure if this helps bu

[Histonet] p16

Hello fellow Histonetters, Does anyone run the antibody p16? If so, where do you purchase it from and maybe you can share your protocol with me? Thank you in advance. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknut...@primecare.org

[Histonet] Region II mtg addiiton and up-date:

Histonetter's, Region II up-date: Save the date for the 2010 Region II Symposium! June 10-12, 2010. Clarion Hotel, Altlantic City, N.J ...Come for a day... or come for the weekend. Excellent Speakers, new workshops, vendor fair and of course...there is always the beach. ..(for studying, of cou

Re: [Histonet] hydrometer

We ordered ours from Fisher Scientific. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net --

[Histonet] hydrometer

Anybody have any idea where to purchase a simple hydrometer for checking the water content in alcohols? I found one at TBS but do not know if it will fit our purposes since it says use with a specific recycler. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervi

Re: [Histonet] Eosin too pink

We add 100mL of 100% alcohol to 150mL of Eosin to tone it down each day.  ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,  AZ ~Don't let your life end before it begins~ OU Rocks From: "Smith, Lesley" To: histonet@l

Re: [Histonet] Helicobacter Pylori antibody

We use Cell Marque's Polyclonal H. Pylori  (CMC435) at a 1:100 dilution on the Ventana BenchMark for 32 minutes, Protease 1 for 12 minutes.   ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,  AZ ~Don't let your life end before it begins~ OU Rocks __

RE: [Histonet] Helicobacter Pylori antibody

We purchase it from Biocare. Protocol: pretreatment Diva (Biocare) 20 minutes water bath 20 minutes cool down. Detection system Mach 4 (Biocare) 10 minutes H2O2, 10 minute primary antibody dilution 1:100, 5 minutes mouse probe, 12 minutes polymer, 10 minutes DAB(Dako), 5 minutes hematoxylin(Dako).

[Histonet] Tissue Tek

I need the 2 parafin pots for a Tissue Tek/Sakura tissue processor working or not . Anyone has them ? Regards Ricky _ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. htt

RE: [Histonet] Helicobacter Pylori antibody

Hi Chakib, We use Ventana's (manufactured by Cell Marque) H. Pylori on their instruments. The protocol is specific to the instruments but involves a routine HIER step and Avidin/Biotin blocking. The antibody itself is ready-to-use but is available from Cell Marque as a concentrate as well. T

RE: [Histonet] Helicobacter Pylori antibody

I have ordered mine from Cell Marque. We use it at 1/50 dilution with a 30 minute incubation at RT on the Dako Autostainers. Works great. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 __

[Histonet] Helicobacter Pylori antibody

Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM ___ Histonet mailing list Histonet@lists.utsouthwestern.e

[Histonet] Please remove me from your list. Thank you

Please remove me from your list. Thank you This message and any included attachments are from ValleyCare Health System and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized rev

Re: [Histonet] Surgical veterinary reference manual

A Google search for "veterinary surgical pathology book" brings up lots! John Kiernan Anatomy, UWO London, Canada. = = = - Original Message - From: Victoria Baker Date: Tuesday, March 9, 2010 9:53 Subject: [Histonet] Surgical veterinary reference manual To: histonet > Hi > > I need to

Re: [Histonet] Butyl alcohol

Hi: I'm not sure if this helps but Botanists have long used tert-butanol (not N-butanol) to embed tissue in paraffin. This classic protocol was popularized by D. A. Johansen in the mid 1930's and involves transition from water to alcohol to t-butanol (I can supply more detailed info if you need

[Histonet] Summer enployment

Hello, I am attending school at this momment, but this coming summer I am looking for part/full time work only for the summer. I am on my way to getting an associates so I can take the histology test. I am a Mohs technician (OHSU) almost 9 years, but was laid off last June, I also do histol

[Histonet] Full Time Day Shift Position

At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday. Must be ASCP registered HT or HTL (or eligible...must become registered within 18 months of hire date). Lab Automation includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We

RE: [Histonet] TGF beta on FFPE

Which receptor? We run BR1,BR2and BR3 on FFPE as well as B1,B2 B3. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Region II Symposium

Histonetter's, Members of Region II and beyond.: Save the date for the 2010 Region II Symposium! June 10-12, 2010. Clarion Hotel, Altlantic City, N.J Come for a day. Come for the weekend. For more information about registration and accomodations contact Michele French: 609-818-3278 / michele.fre...

[Histonet] Surgical veterinary reference manual

Hi I need to locate a reference manual for veterinary surgical pathology - if anyone knows of one would you please contact me? Thanks Vikki Baker ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listin

[Histonet] Eosin too pink

Our Pathologists have, over the last few weeks, complained that the eosin is too pink. We have not altered our procedures or reagents in any way. It seems to be particularly bad in endoscopic biopsies. Thanks Lesley Smith Senior Biomedical Scientist Cellular Pathology The Yorkshire Clinic Bradf

[Histonet] Stainers/Coverslippers

Just a quick thank you to all who responded to my inquiry about stainers/coverslippers...I am bringing all the info to the powers that be so we can start checking out some of these units. I appreciate all your help, Vicki Gauch AMCH Albany, NY - CONFIDE