[Histonet] Mouse brain mitochondrial marker?

Dear everyone, I'm trying to double-label mouse brain mitochondria with my rabbit antibody on paraffin section. I had tried anti-Tom20 (mouse, Santa Cruz) with biotin-streptavidin amplification, but the signal is very weak. So far, I had tried this antibody on cells and it was super-strong. I'm go

RE: SPAM-LOW: Re: [Histonet] Cd31 in pigs

Patsy How do you order that rat anti-mouse CD31. Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorad

RE: SPAM-LOW: Re: [Histonet] Cd31 in pigs

Yea you can use F8 and SMA for vessel lining cells but those will not pick up all the developing endothelial cells like cd31 or cd34. There is a new rat anti mouse CD31 from Japan, I wonder it if works on pig, I will try it and see, it works great on mouse tissue ffpe. Regards, Patsy Patsy Rueg

RE: SPAM-LOW: [Histonet] Re: Cd31 in pigs

A few years ago I spent a lot of effort on using Serotec's mouse anti-porcine CD31 for ffpe porcine tissue and I did get some positive results with unusual pretreatment methods, as I recall I had to use a waterbath for hier set at 70dc for 3 hours in hph buffer. I will look up the method at work o

RE: SPAM-LOW: [Histonet] enzymes significance?

I think Sigma still sells enzyme histochemical kits for Alk.Phos and TRAP, they are listed as Leukocyte acid phosphotase or Leukocyte alkaline phosphotase, contact me at work next week and I can give you the order info as I do still have these kits in my fridge. Regards, Patsy Patsy Ruegg, HT(ASC