Dear everyone,
I'm trying to double-label mouse brain mitochondria with my rabbit antibody
on paraffin section. I had tried anti-Tom20 (mouse, Santa Cruz) with
biotin-streptavidin amplification, but the signal is very weak. So far, I
had tried this antibody on cells and it was super-strong. I'm go
Patsy
How do you order that rat anti-mouse CD31.
Thanks
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorad
Yea you can use F8 and SMA for vessel lining cells but those will not pick
up all the developing endothelial cells like cd31 or cd34.
There is a new rat anti mouse CD31 from Japan, I wonder it if works on pig,
I will try it and see, it works great on mouse tissue ffpe.
Regards,
Patsy
Patsy Rueg
A few years ago I spent a lot of effort on using Serotec's mouse
anti-porcine CD31 for ffpe porcine tissue and I did get some positive
results with unusual pretreatment methods, as I recall I had to use a
waterbath for hier set at 70dc for 3 hours in hph buffer. I will look up
the method at work o
I think Sigma still sells enzyme histochemical kits for Alk.Phos and TRAP,
they are listed as Leukocyte acid phosphotase or Leukocyte alkaline
phosphotase, contact me at work next week and I can give you the order info
as I do still have these kits in my fridge.
Regards,
Patsy
Patsy Ruegg, HT(ASC