A few years ago I spent a lot of effort on using Serotec's mouse anti-porcine CD31 for ffpe porcine tissue and I did get some positive results with unusual pretreatment methods, as I recall I had to use a waterbath for hier set at 70dc for 3 hours in hph buffer. I will look up the method at work on Monday and send it to you if you contact me again to remind me.
Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Wilson Sent: Friday, April 09, 2010 1:44 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Re: Cd31 in pigs Serotec's mouse anti-porcine CD31 (MCA1746) will work well in fresh, frozen, acetone-fixed pig tissues. It is very good for angiogenesis. It does not work with FFPE tissues. Liz Wilson Bethyl Labs ------------------------------ Message: 4 Date: Fri, 9 Apr 2010 11:59:44 -0400 From: "Jean-Martin Lapointe" <jm.lapoi...@accellab.com> Subject: [Histonet] Cd31 in pigs To: <histonet@lists.utsouthwestern.edu> Message-ID: <befd613bd39142499989f836556ddc8394a...@ace.accellab.lan> Content-Type: text/plain; charset="iso-8859-1" Hi Joel, we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had no luck. We currently use an antibody to vWF to stain pig endothelium. If someone has a working technique for CD31 in swine, I'd be happy to hear about it. Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc Quibec, Canada J7H 1N8 jm.lapoi...@accellab.com Date: Fri, 9 Apr 2010 09:40:00 -0400 From: "Joel Israel" <jo...@mcclainlab.com> Does anyone have a procedure that WORKS for cd31 in pigs? I can't seem to get it to work no matter what I do. Any help will be greatly appreciated. Thanks.- joel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet