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Andre,
By looking at your method it does not look right from the start.
We use the Gordon and Sweet Method and it work wonder for demonstration
of reticulin fibers
Here it is Hope it helps
Malika
1. Sections to water.
2. Treat with
I have had one for a few years now and love it. You can also monitor it
from another location if you have analog internet. It is really easy
to change out the reagents and is way less messy than most other
processors. You need to make sure your reagents stay topped off. The
only real problems
Hi All,
We are in need of a used tissue processor. If anyone has one for sale in the
Philadelphia area, please email ros...@email.chop.edu. Budget is very small!!!
Thank you.
Joanne Mauger HT(ASCP)QIHC
Children's Hospital of Philadelphia
From:
Michelle,
We have two Excelsiors and absolutely love them. The tech time saved on
rotating solutions is well worth the cost. Solutions rotate out according
to your specifications or alcohol content. Solutions are loaded during
the next cycle. Tech time saved once again. We also are able to
I spoke with a patient recently who wanted his prostate biopsy tissue (from 2
years ago) tested for Chlamydia. Is anyone doing PCR for Chlamydia on
formalin-fixed, paraffin-embedded tissue? I am reluctant to do IHC testing
because my assay is not validated for prostate tissue. Thank you.
Ashley,
Phosphatases are relatively resistant to fixation which is why the
histochemical phosphatase methods work. You may want to try putting
phosphatase inhibitors in you fix or post-fix solutions to stop
dephosphorylation, and see if it makes a difference in your ihc. I
think Sigma has a
Here is a fixative we found in a paper on Phospho antibodies when we did a
WS at NSH in 2007.
2.0mM EDTA
2.0mM EGTA
2.0mM Vanadate
40mM NaF
20mMpNPP(para-nitrophenyl phosphate)
We used this fixative compared to routine 10% NBF and did not see any
differences in IHC staining with phospho
I have been using the Excelsior for over 4 yrs . Compared to the last VIP we
had this is wonderful!! We save on Reagents by rotating on the first Alcohol
quality. Very user friendly, Would suggest purchasing the Warranty Netmon.
A bit expensive but the PM any parts alone pretty much cover that
Hello histonetter's...I come one again to the experts in the field.
I have been give two HE slides from an autopsy case (have no idea how
old)...and have been asked to retieve this tissue into an eppy with
lysis buffer for DNA. Does anyone out there have an existing method for
doing this? I
Ashley,
When we have worked with phospho antibodies, we have used the following
pre-treatment to induce de-phosphorylation of the tissue sections:
1. _ Deparaffinize slides to distilled water.
2. _ Perform De-phosphorylation step. (Add 400 uL of alkaline phosphatase
(Sigma Type VII-L) to 600 uL
Andre:
1. Red shadows in reticulin maybe as results
too long steps with gold chloride. In your
protocol it is for 5 mins. Try this step as 1 min.
2. Try substitute in step 13) 20% formaline at 10%.
It can be prevent high gray background.
3. Chek pH of DW before nuclear fast red. It must be
approx.
hi histonetters...dont have experience using the supra for cytology
samples...any recommendation???
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If you prefer not to post to the list I'd appreciate hearing about your pros
and cons off list as we will be evaluating this instrument. Thank you Loralee.
Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue Suite 309
Charleston,
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