It's just crazy the wage differences around the country. But we should
fight for the higher wages in whatever location we live in. Not
everyone can do this type of job and be good at it.
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San
Christi
We run a diff stain on all gastric bx suspected of H. pylori as routine, it
saves us time.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
For gastric biopsies we routinely did modified Steiner for H. pylori
René J.
--- On Wed, 5/12/10, Cristi stephenson cls71...@sbcglobal.net wrote:
From: Cristi stephenson cls71...@sbcglobal.net
Subject: [Histonet] Gastric biopsies
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, May 12,
Dear Histonetters,
Does anyone out there have experience with processing whole tilapia fish eye?
We formalin fixed our specimen, dehydrated with EtOH and cleared with Citrisolv
before embedding in paraffin wax. At the microtome we encountered all kinds of
chatter. Any advice is greatly
We do Iron,Trichrome, Pas with and without Diastase and Retic on liver biopsies
if requested by the grossing pathologist. We do Diff-Quik on all stomach
biopsies, and if requested by the pathologist an Hp immno. A tricchrome is done
on colon with a diagnosis of microscopic or collagenous
The simple 1-2% methylene blue solution..(deparaffinize,wash n water,1 dip
m.blue,air dry,xylene,coverslip)..or the DIFF Kit of any brand..
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Histonet@lists.utsouthwestern.edu
I will be out of the office starting 05/13/2010 and will not return until
05/18/2010.
I will be on vacation from Thursday, May 13 through Tuesday, May 18. If you
need immediate assistance please contact Suzanne Mahoney at 800-248-0665
x5440.
HELLO..I wonder what reference lab i can send unstained slide for nk cells
marker like cd 56 etc...im n bahrain..
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We are having an issue with our paraplast (manufacturer McCormick). There are
black flecks and what looks like a dark sand in the paraplast. The issue has
continued for @8 weeks and has happened with several lot numbers. Is anyone
else experiencing this issue?
Sherry S. Hayes
Laboratory
Yes, we had constant trash or sand in ours for months with different lot
numbers, we finally had to change companies
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Hayes,
Sherry
Sent: Thursday, May 13,
Jennifer,
I read my response and realize I made the mistake of stating on all esophageal
biopsies. We only do candida
when the Endoscopy physicians list it on the paperwork. I ran out of our
control for fungus so I am purchasing controls from American Master Tech. (they
use superfrost plus
It's very hard to fight for better wages when the only options are minimum wage
jobs. I was in a similar position as Joelle's in Missouri and had to fight to
be paid over $10/hour after going back to my old job due to a divorce. It was 5
years before my pay was increased to what it had been
Who are you all using to do your PM on your microtomes? Not necessarily
the manufacturer, but an independent contractor.
Thanks.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
Hi,
I need help quickly. Dako, our supplier, for Prion protein clone 3F4
(mouse anti-human), that we use for PrP IHC testing, is not supplying it
anymore .
Mine has expired I have a ?positive to test. I am in Canada, does
anyone who does this testing know of a supplier I could contact.
Any help
I'm not sure where you live but we use a company called DOLBEY-JAMISON.
Phone number is 610-495-2010 or 1-800-220-3073. They operate out of
Pennsylvania. I have found them to be thorough and very good at their job.
Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore
Hello Everyone!
I'm planning to try some IF co-staining with 2 antibodies, one is a
rat-anti-mouse and the other one is rabbit anti-human on a xenograft, each
has an appropriate secondary, donkey anti rat and donkey anti-rabbit,
conjugated to 488 and 593. Can someone advise the best way to
Multiple openings with a reference lab in Teterboro, NJ.
Looking for experienced technologists to work the day shift.
These positions are temporary.
ASCP and NY license preferred.
Contact me today if you may know anyone interested!
Brian Feldman
Principal
Hi Bernadette,
I don¹t know how complicated it would be for you to send something to the
US, but we run CD56 and all sorts of other hematologic markers on a routine
basis...(we¹re a reference lab, too!)
You can find information about specimen submission and the tests we have
available at our
In my experience, most fluorophores are quite stable to many washes. Here is
what I would try at first:
1) Stain with rat anti-mouse and rabbit anti-human overnight at 4C in a
humidified chamber. Dilute each antibody in a single volume of your favorite
staining buffer (PBS, TBS, TBS-T). For
You could try incubating both together (primaries and then secondaries). I
have done this before with primaries of differing isotypes. I know others
have, too.
Regards,
Merced
--On Thursday, May 13, 2010 2:00 PM -0400 Igor Deyneko
igor.deyn...@gmail.com wrote:
Hello Everyone!
I'm planning
I agree with Karen about a decent pay scale for California - $16.15 is a
ridiculous wage for California. Although San Francisco is on the high end
because of our insanely high cost of living (San Francisco even has it's own
minimum wage of $9.75/hr), $16.15 seems horribly low for anywhere in
Next day, pellet cells and resuspend them in a small amount (100-200
microliters) of Histogel or Agar. When the gel cools cut the microcentrifuge
tube open with a scalpel or razor blade. Now you have a nice cell pellet
shaped like the bottom of your centrifuge tube, and you can treat it just
Yes, I do double staining very frequently the way Adam stated. I hardly ever
have to do the sequential staining.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adam .
Sent: Thursday, May 13, 2010
Grumpy old pathologist trying to pay attention to costs:
Livers: if the biopsy was for anything other than metastatic ca, then retic,
trichrome, and iron are pretty much standard. If for met ca, you are wasting
money.
Gastrics for h pylori: Just because the GI guys asked for it doesn't mean
I'm not sure if that was clear looking back on it:
Incubate both primaries together.
Then incubate both secondaries together.
--On Thursday, May 13, 2010 2:46 PM -0400 Merced M Leiker
lei...@buffalo.edu wrote:
You could try incubating both together (primaries and then secondaries).
I have
A growing dermatology/pathology practice in Phoenix seeks a part-time
certified and fully accredited
histotechnologist for its growing pathology business as well as a Mohs tech
for Mohs surgery.
The ideal applicant would be one person competent in both, although
individuals qualified in
I will be out of the office starting 05/13/2010 and will not return until
05/17/2010.
I will respond to your message when I return.
__
This email has been scanned by the MessageLabs Email Security System.
For more information
I completely agree with all Adam has said.
The essential key is to make sure your secondaries are highly cross adsorbed to
each other and including mouse since you are staining mouse stroma. Also run
each as singles alongside your double - that will give you great confidence in
what you are
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