I will be out of the office starting 06/03/2010 and will not return until
06/07/2010.
.In my absence please ask for Mary Campbell . If this is urgent you can
contact me on my cell phone number 858-472-4266.
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Albert:
In histology there are only very few standards. That is why CAP has a check
list when inspecting histolabs.
If you are doing a "validation", get as many different tissue sections as you
can. Stain them "as usual" (namely using the method you are trying to
substitute).
Stain identical sec
We use paraplast Xtra supplied from Leica\Surgipath and have seen no problems
whatsoever.
Stacey Langenberg
Sent via BlackBerry from T-Mobile
-Original Message-
From: Karen Kay
Date: Fri, 4 Jun 2010 11:43:05
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] PARAPLAST QUALITY
G
Good Morning,
Our Paraplast has also been produced by McCormick and to this point have not
seen any issues. However it is my understanding that Leica(Surgipath) has
taken over as supplier and producer of the Paraplast (trademark). This
information was relayed to us by our Surgipath representa
Hello,
Does anyone out there have positive Pneumocystis control blocks that they may
want to trade with? We have some positive GRAM control blocks that we could
exchange. Please let me know. Thank you so much!
Deanne Knutson
Anatomic Pathology Supervisor
St. Alexius Medical Center
(701)-530
No.
Towns are like people. Old ones often have character, the new ones are
interchangeable.
--Wallace Stegner, Angle of Repose
On Fri, Jun 4, 2010 at 11:03 AM, McCormick, James wrote:
> Please unsubscribe my email from your list.
>
> Thank you,
> James B. McCormick, M.D.
>
> ___
Hello fellow Histonetters, I'm going to Demo a new H&E stainer and I was
wondering if anyone knows if there's a standard H&E Validation protocol
or if it just varies from lab to lab? Thanks for your help :-)
Albert Santiago, HT(ASCP)
Laboratory Manager
Dermatopathology
215-662-6759/6539-office
Please unsubscribe my email from your list.
Thank you,
James B. McCormick, M.D.
*** Confidentiality Statement ***
This e-mail is intended only for the use of the individual or entity to which
it is addressed and may contain information that is privileged and
con
We have been using an INI-1 from Cell Marque. With a 1/50 dilution and a low
pH heat retrieval. Although I accidently had a slide go through the higher pH
heat retrieval and I didn't see a big difference in the staining.
I use the Dako system so it was a 30 minute incubation with a linker add
Hi Hazel
We use INI-1, clone 25/BAF47, BD Transduction 612110. Working conc. is 2.5 mg
Ig/l (1:100), HIER at high pH with a somewhat strange buffer (800 mM urea, 100
mM Tris, pH 9.5), but you may try EDTA-based HIER solutions, pH 8.8-9.5.
Incubation at RT, 60 min. Visualization with polymer-bas
We use Richard Allen, Type 6 for both impregnation and embedding.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkb...@chs.net
"Rebecca Johnson"
Sent by: histo
We are having problems with this immunostain. If your lab is
successfully using this antibody will you please help me out?
Where do you purchase your antibody? Is it RTU? What type of antigen
retrieval? Incubation time?
Thanks.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histol
We love our CV5030 glass coverslipper. It integrates with their stainer
for a seamless flow. It's so nice to put the slides on to stain and
have them coverslipped when they come off.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySl
We are currently evaluating coverslippers and I'd appreciate any info/opinions
on the following:
Hacker HCM 6000
TBS SHUR/Mount
Thermo Scientific (Shandon) ClearVue
Tissue-Tek Glas g2
Leica CV5030
We will be looking at stainers within a couple of years so this is something
we need to consider a
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