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I was wondering if anyone on the histonet list is using abCam Olig2 (ab33427)
on the bond max.
I have been trying various titration and enzyme treatment on surgical brain
tissue with not much success al we seams to achieve is nuclear
I had problems with this one too. It now seems that this particular antibody
has been removed from AbCam's product range due to some QC problems.
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From:
Dear Histologists,
Has anyone done TUNEL on 40 um flash-frozen rat brain sections? I have tried
tweaking the protocol (we use the Millipore Apoptag kit - S7101) from our
ongoing 20 um slices by increasing the incubation times from 1 hour to 2 and 4
hours but we still get poor staining. Also,
Gil,
I routinely incubate free-floating 40um rat brain sections overnight or
longer @ 4 degrees centigrade according to the particular antibody that
I am working with. Our sections are perfused in 4% paraformaldehyde and
cryoprotected with 30% sucrose prior to sectioning. How long are you
Hello all,
I am being asked to do immunostaining on fla= t mount sections of rat
eyes. First, what is a flat mount and does an= yone have a protocol
on how to make one? Second, what is the methodol= ogy to do immuno
stains on these? Do you have to fix them, cut them, =
Dorothy Webb wrote:
I would appreciate any feedback on what all are using in your
decalcification process. We get a lot of large bones in and the past 2-3
months have noticed a huge problem in our microtomy process with these
samples. We have been grossing the bones in and leaving the
We recycle formalin, xylene and alcohol and they all turn out very nicely. We
use the CBG Biotech formalin and solvent recycler and then assay/test each
batch and return the formalin back to 10% NBF by adding either distilled water
or formaldehyde depending on what end it came out on and then
