Hi Histonet,
I just would like to thank everyone who responded to my question about
Warthin-Starry stain.
Your help is very appreciated!
Chakib
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I do histology in a small research lab. We use the tissue processor and
embedding station of a nearby lab to process specimens. I would like to
switch to manual in-lab processing of some our more delicate samples like
mouse embryos. I am wondering what histonetters use to store their melted
We receive and process all tonsils. We charge 88304.
Brandi Higgins, BS, HT (ASCP)
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Can you give me a ball park figure for a new paraffin embedding center? I
have a friend setting up a new lab and are working on their budget. They
need a cost of a unit equivalent to the Shandon Histocentre that has the
electric forceps. About 7 years ago I paid about $7000 for a unit such as
We use the low-profile microtome blades and love them. We actually found
Thermo-Fisher's brand of the low-profile to work just as well and they are less
expensive. We had problems with other manufacturers' low -profile blades that
had the oil on them. However we have several sample boxes and I
The oil serves a valuable purpose and shopuld be found on all blades of
high quality. It protects against oxidation and allows the blades to be
easily separated from one another, so the oil has always been there. I
have personally found no advantage to wiping or not wiping the oil from
the
Hello,
I have been asked to to prepare frozen sections of fish livers that have
been stored in RNAlater Solution at -80C. Tissues (about 10 um square x 5
um thick) were embedded in OCT, frozen on dry ice, and sectioned at 6 um.
My problem is that when I cut a section, the center of the tissue
The best blades we've used so far are from Mercedes Medical - MER DMBHPGP - and
are priced well below some competitors blades.
Tresa Goins
Veterinary Diagnostic Lab
Department of Livestock
Bozeman, Montana
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About 1/2 of my tech.'s wipe the oil off; otherwise it causes the first
2-3 ribbons to curl off the knife, rather than forming a nice ribbon.
Sandy Harrison
Histology Supervisor
Minneapolis VA
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