Re: [Histonet] cryostat question

2010-11-01 Thread Alan Bright
Dear Susan, Our Starlet 2212 Bench Top Cryostat has an option for orientation. There are many used for Mohs, as holding a low temperature is not a problem. I can email you some user references if. You want. Regards: Alan Bright Bright Instrument Co. Ltd. England Sent from my BlackBerry®

[Histonet] FW: I love this story

2010-11-01 Thread Doughty, Adele
Good morning sure was getting to talk to you the other day. This past weekend was the first time I heard dad complain that he did not feel good, headache and nausea. His hair is getting thinner. Not much else happening here thank goodness. Love ya, A

[Histonet] Pax 8

2010-11-01 Thread Rae Staskiewicz
Does anyone know where to purchase Pax 8 antibody for formalin fixed paraffin embedded tissue? Rae Staskiewicz Methodist Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] RE: Medite Microtomes

2010-11-01 Thread Nails, Felton
I have had some experience with this company and their embedding station but not microtome. The small cold area where you orient the tissue tends to ice over. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

[Histonet] aluminum gutter guard

2010-11-01 Thread DiCarlo, Margaret
Hello Histonetters, I have been using aluminum gutter guard to wrap my bones when I manually process them- from increasing percentages of alcohols to xylene and paraffin. I am running very low on it and I have not been able to find it at any hardware stores. I looked on line and Amazon is out

RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 46

2010-11-01 Thread Feher, Stephen
http://www.cytopathnet.org/tiki-index.php Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miranda D. Felton Sent: Saturday, October 30, 2010 1:43 PM To: histonet@lists.utsouthwestern.edu Cc:

[Histonet] Nashville TN Ht Position

2010-11-01 Thread Hale, Meredith
Great opportunity for a Histotechnician in a brand new laboratory! Bellmeade Dermatology in Nashville, TN is looking for a certified HT or HTL to run their newly constructed laboratory. Bellmeade Dermatology has been in the dermatology business for 18 years with 3 physicians and 2 Nurse

RE: [Histonet] Pax 8

2010-11-01 Thread McMahon, Loralee A
Biocare, They have a prediluted. Works great. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] IHC Staining Problem

2010-11-01 Thread Reynolds,Donna M
We had a similar problem a few years back. We had used an antibody at 1:500 dilution for years. Got in a new bottle and no labeling. Called the manufacture and they assured me it was the same concentration. Finally went to 1:50 dilution and the staining was like our old stain. Over the last

[Histonet] Standard work

2010-11-01 Thread Marcia Funk
Hist We are looking at tracking system and would like some additional data from labs that are tracking standard work for sectioning, embedding, labeling. Also special stains and IHC would be helpful. Like to compare our lab with other labs to see if we are in the

RE: [Histonet] Standard work

2010-11-01 Thread Mahoney,Janice A
Hi Marcia, I'd be glad to help but could you be more specific as to what you need? We have the Ventana Vangage system and love it. We are a Lean Lab as I know you are and I think this system fits in perfectly. Please feel free to contact me for specifics. We have Job guidance sheets (i.e.

RE: [Histonet] Standard work

2010-11-01 Thread Kuhnla, Melissa
Hi Marcia, We are a core lab...doing the work of four hospitals. We have been live with Vantage (Ventana) for about nine months. We have 3 symphonies, 3 ultras, 1 XT, and two nexus special stainers all tied into the system. I would be happy to answer specifics for you. We are happy. Ventana

[Histonet] Frozen Section TAT

2010-11-01 Thread Scott, Allison D
Hello to all in histoland. How is everyone calculating the FS TAT. Does any anyone have a file that automatically calculates that tat when you plug in the data, that they would be willing to share. Any help would be appreciated. Allison Scott Histology Supervisor LBJ Hospital. CONFIDENTIALITY

RE: [Histonet] Frozen Section TAT

2010-11-01 Thread Rathborne, Toni
We have recently started using our LIS to help with this. When the specimen is accessioned (in Cerner Millennium) as a frozen section, tasks are generated. Additional tasks can be added depending on how many slides or additional blocks are prepared for the FS. These tasks can then be verified

[Histonet] RE: Frozen Section TAT

2010-11-01 Thread Weems, Joyce
Our pathologist dictate the times and we review it manually for 100% of the frozens!! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From:

Re: [Histonet] Frozen Section TAT

2010-11-01 Thread Victor Tobias
Allison, We have 2 fields in the database for when the frozen specimen was received and when the surgeon was notified. We can then run a report to show us the cases with frozens and the TAT. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of

[Histonet] IHC Staining Problem

2010-11-01 Thread Amos Brooks
Hi, I think you mentioned using citrate retrieval. Have you tried high pH retrieval with EDTA or an equivalent? I think CD79 works better with high pH retrieval. Amos Message: 1 Date: Sun, 31 Oct 2010 19:29:59 + From: Jennifer Hill jh...@vet.k-state.edu Subject: RE: [Histonet] IHC

RE: [Histonet] IHC Staining Problem

2010-11-01 Thread Ross Benik
We have developed a staining protocol for the CD79a antibody from two different vendors and both require a HIER TRIS/EDTA pH 9.0 retrieval. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent:

Re: [Histonet] Frozen Section TAT

2010-11-01 Thread Rene J Buesa
Under separate cover I am sending something on this subject. René J. --- On Mon, 11/1/10, Scott, Allison D allison_sc...@hchd.tmc.edu wrote: From: Scott, Allison D allison_sc...@hchd.tmc.edu Subject: [Histonet] Frozen Section TAT To: histonet@lists.utsouthwestern.edu Date: Monday, November 1,

[Histonet] HT/HTL Opening Tualatin, Oregon

2010-11-01 Thread Konni Black
An experienced HT or HTL is needed for a new histology lab in Tualatin, Oregon. Great company, very flexible schedule, excellent pay. Please contact Konni Black if you are interested. kbl...@digestivehlth.com or 253-503-2560. ___ Histonet mailing

Re: [Histonet] Frozen Section TAT

2010-11-01 Thread histot...@imagesbyhopper.com
Allison, I have created an excel spreadsheet where you put time started, time completed it will calculate the amount time spent doing the frozen. It highlights any time grater than 20 min. Let me know if you are interested in something like that. Michelle On Nov 1, 2010, at 2:54 PM,

[Histonet] THANK YOU !!!!

2010-11-01 Thread Lin Bustamante
To all of you for the answer to my questions concerning 100 um thick plastic sections and Absolute v/s absolute+methanol. Thanks again Lin. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station,