[Histonet] Frozen section ...HELP

Hi all, after more than a decade of NOT cutting frozen sections, I find myself back at the ice-face. To get my hand in (not literally) I thought I would do some trial sections on stored tissue - stuff that was in formalin and now in 70% alcohol. Horror ; dismay. The tissue, once frozen, is all

Re: [Histonet] Frozen section ...HELP

The freezing point of water is 0 degrees C. The freezing point of 100% ethanol is -114 degrees C. The freezing point of 70% alcohol is about -48 degrees C. Since most cryostats are at -20 to -25 degrees C, your tissue isn't freezing completely. You probably have slush ice inside the cells. Not

RE: [Histonet] shrinkage during IHC

In my experience, IHC usually plumps tissue back up during reteival. Is this noticed with every antibody or just a few? Certain tissue types? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

RE: [Histonet] Frozen section ...HELP

Hopefully you removed the antifreeze (alcohol) before freezing? :-) I have run into this a few times. I work in a core facility where people send me samples from all over. Recently someone sent me some samples fixed in Histofix, which is a commercially available aqueous fixative. But they

[Histonet] Distance Learning

We are thinking of using one of the online or distance learning programs to help in the training of new histotechs. Has anyone had any experience with any of these programs, what colleges offer the program, pros, cons. Any feedback is welcome. Thanks  Phyllis Thaxton HT(ASCP)QIHC DCH Regional

[Histonet] Microwave processing effect on DNA/RNA

Hello All, I have seen some posts on the possible damaging effects on DNA and RNA by microwave processing. Does anyone have a reference article that they can share with me? We are in the process of budgeting for next year and we are looking at microwave processors; but I want to verify if this

[Histonet] Re: 8. Distance Learning (Phyllis Thaxton)

Hi Everyone, I'm new to histonet and haven't participated in any discussions yet, but I noticed the question regarding distance/online programs for histotechnicians and I'm currently enrolled in an accredited Histology Technician program through Columbus State Community College. I'd be more

[Histonet] RE: Frozen Section Help

Did you cryoprotect the tissue before you cut it? JB Jo Ann Biedermann Research Assistant University of Arkansas for Medical Sciences Reynolds Institute on Aging 629 Jack Stephens Drive Room 3173    Mail Slot 807 Little Rock, AR 72205 Phone: 501-526-5803 FAX: 501-526-5830 jabiederm...@uams.edu

[Histonet] Re: Microwave processing effect on DNA/RNA

Matt, You asked an interesting question and it got me to googling. Since microwave radiation is non-ionizing it should not adversely affect things like DNA and RNA. I found this summary of a group of publications on this and apparently in 1995, Kakita demonstrated that microwaves were capable

[Histonet] p53 cytology controls

We receive requests from our pathologists for p53 IHC on urine but I am unsure as to what controls to use. We currently use a FFPE tissue control (I understand that it is not correct) but up until now this issue has not been addressed. Can anyone help with good cytology controls? Thanks.

Re: [Histonet] p53 cytology controls

Hi Justin, Do you run the p53 on a cell block or a cytospin or something different? Mark On Mon, Nov 8, 2010 at 12:36 PM, Justin Peters jpet...@bostwicklaboratories.com wrote: We receive requests from our pathologists for p53 IHC on urine but I am unsure as to what controls to use. We