You did not say WHICH plastic?
Immunostaining on tissues embedded in methyl methacrylate are successful
since one can totally remove this plastic from the tissue with various
solvents. There may be some stringent antigen retrieval needed . Neil Hand
had a panel of over 200 antibodies wit
for those of you that are doing her2 immunos, are you charging more for these
than for other antibodies? I pay way more for these, and it seems we should be
able to charge more? thanks, everyone have a great weekend!!
anita dudley
providence hospital
mobile, alabama
Hi Angie,
Jan M has nailed it. Have a good weekend. -
William (Bill) O'Donnell, HT (ASCP) QIHC
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthweste
Check the MSDS, they should contain storage guidelines. Just follow them
René J.
--- On Fri, 1/21/11, Laurie Colbert
wrote:
From: Laurie Colbert
Subject: [Histonet] Storing Acetic Acid
To: histonet@lists.utsouthwestern.edu
Date: Friday, January 21, 2011, 3:48 PM
I am trying to find out how
I am trying to find out how to store acetic acid. We were storing it in
our acid cabinet with the other acids, but we were inspected by the fire
department last week and they said that acetic acid is flammable and a
corrosive and that it should be stored with the flammables. But my
manager is say
Hello Everyone!!
I am looking for Two Histotechnologists to perform various routine duties
and/or highly complex analysis (immunoperoxidase and cutting tissue) within the
Histology Section of Pathology and Laboratory Medicine at a hospital in
Columbia, SC. Work hours are 8a-4:30pm M-F, no week
Hi All,
There is a part-time opening in a great new lab in sunny Scottsdale, AZ..
Licensed HT or HTL must have 3 or more years experience and be able to work
independently. Great pay, very flexible schedule.
Please contact Konni Black for more details. Thank you for your interest.
253-503-2560
I have worked in several labs where lab assistants were embedding. They were
even doing it in the midst of CAP inspection. It was not an issue. Proper
training documentation was done and each block was cut by an experienced tech.
As a matter of fact, in one lab it was so basic that symbol
Hi Jan
IHC on plastic-embedded specimens is difficult, I think the antigens can get
rather damaged from the embedding and/or deplastifying processes. In our
experience, it's possible to do some markers, while others are irretrievable.
One thing I notice is that our protocol for deplastification
I recently had an animal from a previously undescribed mouse model die
suddenly during the weekend. Since I couldn't send it off to necropsy, I
stashed it in the refrigerator until Monday morning. According to the person
who read the histology "Post-mortem degeneration limited evaluation of most
ti
Dr. Cartun,
We are using the C3d and C4d from Cell Marque. On the Dako platform.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwe
If the tissue is not fixed soon after death the microscopic morphology
will be terrible, no matter whether the animal is refrigerated or
frozen. The person suggesting refrigeration/freezing is (obviously) not
familiar with histological technique.
As someone else on the list suggested, better pla
My apologies! Please use this corrected link to learn more about becoming a
MediaLab author for histology courses, visit our online information page at
www.medialabinc.net/authors.aspx . Please contact Judi Bennett at
j...@medialabinc.net or call 877-776-8460, ext. 721.
--
Judi Bennett, BSM,
Actively seeking authors to write and review online *histology courses* for
*MediaLab*! MediaLab is a leading publisher of online continuing education
(CE) courses and competency assessments. Our online products are used at
more than 2,000 laboratories and university CLS programs worldwide.
This
Great opportunity for a Histotechnician in a brand new laboratory!
Avamar Gastroenterology in Warren Ohio is looking for a certified HT or
HTL to run their newly constructed laboratory. Candidate must be ASCP
certified and CLIA certified to perform gross dissection, prior
supervisory experience pre
As long as you document guidelines, training and continued competencies
you will be okay.
-Original Message-
From: Angela Bitting [mailto:akbitt...@geisinger.edu]
Sent: Friday, January 21, 2011 9:14 AM
To: Janice A Mahoney; Mike Pence;
histonet@lists.utsout
Hi Histonet Members,
I am currently working on a Laboratory Manager position in MIY. the ideal
candidates must have strong supervisory/management experience, be HT ot
HTL(ASCP certified, and have strong experience in dermatopathology. This job
opportunity is with a leading national diagnos
Yes, I wondered about that reference too.
This is embedding, not grossing that I'm asking about.
>>> "Mike Pence" 1/21/2011 9:49 AM >>>
Why, ANP11610 has to do with Gross Examination Qualifications for techs
doing grossing.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.e
Kirshna,
I wouldn't consider this to be a good practice. I would expect that if the
experimental study is important, thought and planning would have dictated that,
at time of necropsy, enough resources would be available to properly cover the
necropsy procedure. If an animal unexpectantly die
Why, ANP11610 has to do with Gross Examination Qualifications for techs
doing grossing.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Rathborne, Toni
Sent: Friday, January 21, 2011 8:34 AM
To: Mahoney,Jani
I'm wondering if anyone has experience with immunohistochemistry using plastic
sections. I am currently working with 4um sections, and have tried doing
staining with and without Dako antigen retrieval, but I am not getting very
good results. Tissues (mostly mouse bones, but some soft tissues a
If you are a CAP inspected lab, be sure to determine if your lab assistants
qualify by meeting the guidelines in ANP11610.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of
Mahoney,Janice A
Sent: Friday, Janua
As long as you are in a state that does not require licensure for Histo techs
you are fine. Just be sure that you have training and competency performed and
documented according to CAP. We have done this successfully in my lab.
Jan,
Omaha, NE
-Original Message-
From: histonet-boun...@l
Hello Ever-helpful Histofriends,
I want to train my lab assistants to embed simple tissues like breast
resection specimens, placentas, etc.
My manager feels that this will break some kind of regulations and won't sit
well with our doctoral staff.
I feel pretty confident that other Labs are do
I am not a CAP inspected lab, but I do QC my ventana detection kits in
two different ways. They are both very simple and manual. Both came to
be because of faulty detections kits and actually events we experienced.
1. Load the detection kit on a wedge and manually dispense each
dispenser onto a pa
Dear Group Members,
I have a strange query, One person suggested, if the animals for necropsy are
more and one can not handle the load of necropsy on the same day.
Then simply after sacrificing the animals refrigerate the dead animals to
reduce the autolysis process and then proceed with the
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