[Histonet] Beta Galactosidase antibody

Hi Everyone, Does anyone have a favourite antibody for beta Galactosidase that they would be confident to recommend? Thanks a lot. Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's

[Histonet] Over process prostate biopsies tissue?

Does anyone have any solutions on resolving over process prostate tissue. What different kinds of reagent and program for your processor that you used to help with the problem. Lovetta M Adams ___ Histonet mailing list

[Histonet] RE: Beta Galactosidase antibody

Hi Margaert, Try this one from Aves lab: http://www.aveslab.com/products/epitope-tag-and-gfp-antibodies/b-gal-b-galactosidase-lacz-antibody/ I did not try them on paraffin, but it worked fine on formaldehyde-fixed cells and cryo-sections from formaldehyde-fixed embryonic tissue. Anatoli

[Histonet] RE: dual fluorescent staining

Hi Ross, Use sequential staining with monovalent anti-mouse Fab fragments (donkey or goat) from Jackson ImmunoResearch labeled with different fluorochromes - DyLight488 and DyLight594, for example. As blocking step I use 5% normal donkey serum. Usually it works fine. Anatoli Gleiberman, PhD

[Histonet] HT Position

Great opportunity for a Histotechnician in a brand new laboratory! Avamar Gastroenterology in Warren Ohio is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience

[Histonet] NYSHS Call for Award Nominees

Every year, the New York State Histotechnological Society presents our colleagues and peers with awards that recognize their achievements, commitment and professionalism. We are very excited to have 7 student scholarships, 1 mentoring award as well as 2 registration awards. Please visit our

RE: [Histonet] Cytoplasmic staining with Ki67 in frozen sections

James I believe the nuclear staining pattern that you describe is normal for Ki-67 in the spleen, not sure about the cytoplasmic or particulate staining. Since you viewing with fluorescence the particulate staining may be autofluorescence of red blood cells. Normally our primary antibody

[Histonet] Rat Skin

Can anybody share their protocol on processing rat skin I seem to be having a problem with it being under processed. I fix the tissue for 11hrs in zinc formalin and then run a 12 hour processing schedule the skin size is 2.5cmx1cm. Thanks, Sharon ___

[Histonet] RE: Rat Skin

The section of skin is too large. It should be no thicker than approximately 3mm. If you need to see the entire piece of skin, you should be able to submit multiple thin sections. A standard 12hr processing schedule works. I am not familiar with zinc formalin, but the standard fixation time

[Histonet] RE: Cytoplasmic staining with Ki67 in frozen sections

Dear Sonya,Acetone is an excellent fixative for many antibody to stain frozens, however nuclear antigens like Ki67 after acetone fixation get a bit fuzzy. I would strongly recommend to fix the slides 5 min at room temp in standard 4% buffered formalin, wash with PBS (or TBS) and start your IHC.

AW: [Histonet] Oil Red O for FS on Muscle

Hi Akemi, we don't do Oil Red O, but the Sudan III in an aceton-ethanol mixture. To prevent the precipitates on the slide, we stain in a plastic coplin jar with a screwed tap. The dye-precipitates accumulate on the ground. We take care not to mix them up while putting in the slide. So we need no

Re: [Histonet] Bacterial contamination

One place you need to look is the floatation bath where you cut your slides. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper

[Histonet] Paraffin

HI all Just wanted to get peoples opinion's on the paraffin you use for processing animal tissues. I am interested in why you use a particular type, pros and cons. This query would apply to mouse and rat but I would also be interested in other species specific information if you have. You do

RE: [Histonet] Paraffin

I have always liked paraplast. It's good because you don't seem to have as much tissue separation from the paraffin in the block. It also seems to hold up longer on the water bath to give sections a chance to de-wrinkle themselves without having to pull so much with forceps. It also doesn't

[Histonet] GSH meeting - hotel deadline

Hi Georgia, Alabama, ALL histotechs, The Georgia Society for Histotechnology invites you to our meeting March 25-27, 2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. and very convenient to Alabama folks, so come across the line. The invitation extends to any

[Histonet] Bacterial Contamination

Thanks to everyone that sent in their suggestions. I especially liked the one that suggested I inform all my pathologists that special stains are unconstitutional. Seriously, though, I'll give all your suggestions a shot and see if I can get rid of the little rascals. Thanks again! Sally

[Histonet] Oil Red O for FS on Muscle

Hi Akemi, Order the stain from Electron Microscopy Sciences - cat # 26503-02. It comes as 250 ml solution and is a lot less expensive than some other sources. I guess you can probably get it through VWR. Mix 3 parts of this stock with 2 parts dist. Water and let it stand for 10 min.

[Histonet] Oil Red O for FS on Muscle

Few more things: 1. The mixed/working solution is good for 1-2 hours only. If you filter it for a long time, this might use the good working time and then you have trouble, because it just won't work well for you. I just poor the stain and the water in a glass cylinder and shake it.

Re: [Histonet] Bacterial contamination

Another place to check is the holding water that the slides rest in just prior to staining. We run our slides to water on the HE stainer and then transfer the rack to a holding water dish. We bleach this dish nightly, as we have found it contaminated in the past. We don't know what caused

[Histonet] Cytology vs. Histology

I am a Cytotech that now functions as the Pathology Manager over both Histology and Cytology. There is no easy answer and it totally depends on the work environment. At our hospital, the Cytotechs also prep their work, and we do no GYN cytology. So they spend an equal time between bench work

[Histonet] PROCESSORS

We are looking at purchasing a new processor. I would like any feed back negative and positive for the Shandon Excelsior ES vs. the VIP 6. Thanks you. You are always so helpful. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold

[Histonet] RE: Bacterial contamination

Sally, It is possible that the bugs are coming from the sectioning water bath. We found that if sections for microbiological staining were cut late in the day, the critters appeared over the slide (not up near the slide label). The controls, which were cut as a batch early morning did not

Re: [Histonet] PROCESSORS

I always recommend ANY VIP over ANY other. My experience with several makes me do it. René J. --- On Thu, 1/27/11, Kathy M. Gorham km...@grh.org wrote: From: Kathy M. Gorham km...@grh.org Subject: [Histonet] PROCESSORS To: histonet@lists.utsouthwestern.edu Date: Thursday, January 27, 2011,

[Histonet] FOXP1

I'm wondering who in IHC land may be using FOXP1? Are you using the polyclonal or the mouse monoclonal (which clone)? Why do you prefer one over the other? Any advvice will be more than welcome. Thank you. Debbie Nannenga, HTL(ASCP), QIHC InCyte Pathology Spokane Valley, WA 99216

[Histonet] Invitation to connect on LinkedIn

LinkedIn Douglas Gregg requested to add you as a connection on LinkedIn: -- Jackie, I'd like to add you to my professional network on LinkedIn. - Douglas Accept invitation from Douglas Gregg

RE: [Histonet] PROCESSORS

I demoed the VIP 6 and it is a nice machine. I ended up purchasing the ASP 300 from Leica. We liked it so much we are purchasing a second one this week. My 2 cents worth! People are not an interruption of our business. People are our business. Stacey Langenberg HT (ASCP) QIHC Laboratory Manager