Hello, Robert, Michael and all those keeping an eye on this list,
Thank you very much for your fast (instant, I'll say, taking into account
our respective timezones) answer to my question.
Best regards,
Jorge Tornero
Instituto Español de Oceanografía-Cádiz
Spain
www.ieo.es
2011/1/28 Robert Rich
Ada Feldman sent me an article on Oil Red O staining and I wrote the
following protocol for my lab based on the article. It works exceptionally
well, frozen adrenal controls have brightly stained positive cortex and
clear negative medulla. Best of all it has no precipitate like all the other
met
I think it is a very small minority of markers that would be sensitive to the
methanol residues present in formalin. So it depends on what you plan on
staining. For general purposes, I would always go with formalin, unless there
is a proven need for formaldehyde.
__
Gilson - whoever he may have been - the name is probably French,
pronounced zheelSAW - apparently published both mercury and zinc forms
of the fixative, more than a century ago. My source is the venerable
Microtomist's Vade-Mecum, 11th ed. 1950, pages 40 and 43.
The mercury formula can be convenie
Great opportunity for a Histotechnician in a brand new laboratory!
Avamar Gastroenterology in Warren Ohio is looking for a certified HT or
HTL to run their newly constructed laboratory. Candidate must be ASCP
certified and CLIA certified to perform gross dissection, prior
supervisory experience pre
We have had this problem occasionally too, but I must say, not with the same
frequency we had with the Benchmark XT. Faulty heaters were replaced next
working day.
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message
Hi,
I see this issue from time to time. One thing to note, does the same heater
stay out or do they jump around? If they jump around, this is probably due to
an issue with the power for the instrument. Make sure all your line
conditioners and battery backups are performing properly and this
Jan,
Depends on your material, further processing and antigens. Surprisingly, some
antigens are better preserved after formaldehyde fixation than after buffered
formalin (probably, due to high sensitivity of particular epitope to alcohol
traces from standard formalin solution). I know at least o
Hi Nancy,
For labeling and identification our company provides a LEAN solution that
allows you to print-on-demand using barcodes from cassettes, specimen
containers, or requisition forms. This can eliminate batch processing,
keyboard entry, handwriting, and adhesive labels from your workflow.
Is there a difference between using paraformaldehyde and neutral buffered
formalin when choosing a fixative for IHC? I would prefer to use formalin
because of easier preparation, but am willing to put in the extra time to make
fresh paraformaldehyde solution if there is a compelling reason.
Ja
Jorge Tornero asks about Gilson's fluid.
The original Gilson's fluid was made up of Mercuric chloride 2 grams, glacial
acetic acid 0.4ml, nitric acid 1.8 ml, 95% ethyl alcohol 10 ml, and distilled
water 88ml.
I don't know about usining zinc chloride in the formula
Michael Titford
USA Patholo
Sorry it would not go through for me on a regular post. Pam
Good Morning,
We have two positions open in Little Rock, Arkansas at UAMS. I will preface
this by saying we are not able to pay relocation or other bonuses so please be
aware of this before answering. I will also state we ar
Our 2 closed system processors are located under a hood. We do a remote
drain and fill. This is all completed under the hood.
Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590
Speak only well of people and you need never whisper
Curt
We fill our bottles in the hood. They are emptied into a vented
flammable cabinet that contains a 55 gallon drum. You know you can do
your own chemical badges for both xylene and formalin. I like to run a
couple TWA (8 hour) exposures and then I badge a lot of STEL (15
minutes) exposures o
Yesterday I asked for some direction on ventilation firms to evaluate my lab
and the fumes. I think I've got some decent leads but I have another quick
question, does any change the processor bottles, formalin, alcohols,
xylem/subs, inside a ventilated hood?
Thank for your input,
Curt
I remember a post about a dermpath job opening. Is this still
available?
Thanks,
Nacaela Johnson
Histology Technician
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office: 913-234-0576
Fax: 913-433-7639
Email: nacaela.john...@usoncology.com
The contents of this electron
Hi Histonetters!
Did you make a New Years Resolution? How is it going so far?
Of course I resolved that I will try to eat right and exercise and I am
trying.
But
my most important resolution is to help even more histo techs find
the right opportunity in the right place at the right time.
1-Make sure that your instruments are located in the same sequence as your
procedure allowing the readily transfer of specimens between them.
2-Get a tissue processor and processing protocol that will allow you to spend
the same time preparing and processing the specimens, as the time it takes yo
Still have an immediate need in the East Bay of San Francisco for a histotech.
Must have hospital experience. Hours can be flexible. Ongoing contract
position. Great opportunity!
Contact me today for more details!
Heidi Hawthorne
Sr. Account Executive
On Assignment, Inc.
t: (510) 663-8622
Hi Folks,
Someone from Leica should answer this question for you (Sara, are you listening
in?), but I have a Bondmax and from time-to-time one of the heating pads will
burnout. The other 9 positions continue to work. It used to be that the whole
assembly was automatically replaced but now they m
We have a Bond III that's about a year old. I'm also interested in the answer.
What does Leica say is the normal replacement period for this item? Is it
covered under your service contract?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.
Has anyone had any problems with the Leica Bond Max's
Heater assembly. We have 2 Bonds that are less than
2 years old and one of the heater assembles went.
That is 10 less slides that we have to work with.
Thank you,
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
973-972-5232
-Original
Hi Nancy,
We built a brand new pathology lab from the bottom up last year. Drop
me an email off line or give me a call and we can discuss our lessons
learned etc. I worked with a LEAN workflow specialist on the design and
equipment placement prior to the plans being approved. We included the
ar
Hi Histonetters-
I am looking for input, pros and cons or anything else you can offer. We are
designing a new histology lab to move to and I am interested in how others have
handled this along with using LEAN processes. What would you do differently?
What would you do the same? Are you in th
LinkedIn
James Dooley requested to add you as a connection on LinkedIn:
--
Jackie,
I'd like to add you to my professional network on LinkedIn.
- James
Accept invitation from James Dooley
http://www.linkedin.com/e/yvpgd1-gjh624wd-5a/qXtGZ0-QiF7
Do you know if people inflate with OCT and then successfully get protein
out of the tissue or look at collagen via hydroxyproline assay? I know
we have been able to extract mRNA but not sure if the gycols in oct
would have any effect on getting protein out to look at collagen via the
assay mention
Hi Guys,
I am sure that there are New Orleans histonetters out there. I am going there
in a couple of weeks for a few days with the kids (boys, age 9 and 14). I was
wondering if anyone could suggest some good places to go, for both kids and
grownups (we are going with other families and have
Hi An,
You did not mention a clearing step (xylene, or xylene substitute) between your
alcohol and paraffin steps. Maybe you just forgot to write it, but if you
actually missed this step, your samples would be improperly processed leading
to the problems you have.
Nancy Thomas
Stowers Institut
Dear collegues,
I am experiencing following problem.
I have embedded hypothalamus tissue in paraffin using the following procedure:
-fixation in 4% paraformaldehyde for 48 hours, fixation of the tissue in 50%
alcohol, next day in 70% alcohol,next day paraffin embedding.
During the paraffin embed
Hello all,
Does anyone know about a Gilson's fluid formula which is mercury-free? I've
been searching and I've found a paper on it in Histo-Logic Vol VI, Num 4,
October 1976 by Carolyn A. Barsczcz who reccomends the use oz zinc chloride
in substitution of mercury chloride in zenker type fixatives
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