RE: [Histonet] fixation question for IHC

It is possible to freeze paraformaldehyde in aliquots, so you don't have to keep on making it up repeatedly as we did in the old days. I have also seen that you can buy Pure formaldehyde, but I don't remember who the supplier was. It was in sealed ampoules and was 16% so you could dilute it in

RE: [Histonet] Immunohistochemistry slides drying and baking protocol

Good Mornig, We currently dry slides at room temp for 15 minutes then bake for 2 hours at 60 degrees. Works well. Melissa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gomez, Milton Sent: Sunday, January

[Histonet] NYSHS Call for Award Nominees

Submission deadline extended until March 31, 2011! Every year, the New York State Histotechnological Society presents our colleagues and peers with awards that recognize their achievements, commitment and professionalism. We are very excited to have 7 student scholarships, 1 mentoring award as

[Histonet] IHC Reference Resources

Hi to everyone in Histoland, I'm working on beefing up my resource library for IHC. What are some publications that you just couldn't go without? Which provide the most complete antibody information that is on the market today? Thanks for your input! Randi Hayes Histology Supervisor, The

RE: [Histonet] IHC Reference Resources

I love the website nordiqc.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hayes, Randi (HorizonNB) Sent: Monday, January 31, 2011 9:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC

RE: [Histonet] fixation question for IHC

I routinely freeze Karnovsky's fixative (paraformaldehyde and glutaraldehyde mix) in small aliquots. When you thaw, just vigorously shake to make sure all is in solution. Have had no problems with fixation. Peggy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] New York Histology Jobs

Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career

[Histonet] Immunohistochemistry slides drying and baking protocol

Hi Milton, We've begun baking ALL IHC slides for 2 hours at 60 degrees C. This also applies to unstained extra slides that may potentially used for IHC, including extra sections on breast cores, lymph node cores, cytology cell blocks etc. No specific room temperature drying time before the

[Histonet] Dog Cartilage Safranin-O Stain

I am staining the joints of multiple species with Safranin-O/Fast Green/Hematoxylin and consistently see a much weaker stain with the SO/FG in dog cartilage. At first I thought it had to do with the thickness of the sections, but even as 6u it's still very light. I'm staining it at the same time

Re: [Histonet] IHC Reference Resources

In my library is: David Dabbs Diagnostic Immunohistochemistry: Theranostic and Genomic Applications which is one of the best books, Jules Elias Immunohistopathology: a practical approach to diagnosis Taylor and Cote Immunomicroscopy: a diagnostic tool for the surgical pathologist Shan-Rong Shi

[Histonet] Hematology stainers

Hi Histonetters, do any of you out there stain the peripheral, aspirate and touch prep slides of bone marrows in the histo lab? If you do, do any of you use an automated stainer and if so, what kind of stainers are being used? I have quotes for a Wescor Aeorspray Stat Slide stainer, a QuickSlide

[Histonet] Chromogenic ISH with riboprobes service

Can anyone recommend a laboratory or company that does ISH service using chromogenic detection with riboprobes we supply on slide mounted cryosections? Thanks for your help, as always. Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research

[Histonet] RE: Hematology stainers

I thought I was the only histology lab that got stuck staining bone marrow smears. I started with staining smears manually, then went to the hematek and finally discovered the Wescor stainer. The wescor stainer has really brought consistency to this area. The only potential problem is you have

[Histonet] Collagen X

Hello again everyone, First of all, just wanted to say thanks to everyone who provided advice to my dual fluorescent stain question, it was all very helpful. Now, I am wondering if anyone knows of a good positive control tissue for Collagen Type X IHC staining? Thanks in advance, Ross

[Histonet] Looking for a procedure

Looking for a procedure to embed tissue culture cells (speficially algae) in agar for frozen sections. Anyone have any? Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center 1900 N. 42nd Street Lincoln, NE 68583-0907 voice (402) 472-1434 FAX (402 472-3094

[Histonet] charge question (sorry)

I hate to bring up charges again but I was asked to find out how much is being charged for a HE on a slide that is already cut. Thanks! Andi Grantham___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Marvin Hanna

Hi All, Sorry to do this on the list but if Marvin Hanna is out there, could you contact me off line. Thanks Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369

Re: [Histonet] IHC Reference Resources

Patrick, Your list is valuable advice: buy some books for the lab. This is the best possible investment. People who understand their work quickly solve problems and shoot troubles. I know most of the books in your list, and agree with your recommendations. BUT: The first item (Dabbs) has