Hello,
I'm trying to cut undecalcified bone (mouse knees) by using the tape
transfer method.
The problem is that the area that I'm interested in (the joint with
patella) is not transferred from the tape on my slide.
I've used slides with different coating (also those specially made for
Dear friends of Histonet,
I have send already a message last week considering problems with thionin
staining. I got a lot of protocols, thank you for that. We used different kind
of solutions, but the problem remains, independently of the solution: when our
samples go through the ethanol (50%,
Hi Histonet-
I recently ran a PAS/D stain and had some issues with it. Both with and
without slides came out looking the same so I'm guessing my digestion step
didn't work! I used a Malt Diastase solution (0.5g to 500mL water) for my
digestion. This is the procedure I used:
1.
You're not kidding Claire!
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
A
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Lee
Mayhew
Sent: Tuesday, February 08, 2011 4:55 PM
To: Histonet
Subject: [Histonet] Cassette labeling
Hi Histonetters,
At my hospital, we are having a
B
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC 29406
843-847-4586
843-847-4296 fax
This email and any files transmitted with it may contain PRIVILEGED or
CONFIDENTIAL information and may be read or used only by the intended
B.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkb...@chs.net
Lee Mayhew lmayh...@cogeco.ca
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/08/2011
We are looking to get a bar-coded system in our laboratory. We currently hand
write all cassettes and slides. What labelers do you have and do you like
them? Also does anyone have the Ventana Vantage system? If so, would you
recommend Vantage?
Carol Bryant, CT (ASCP)
Cytology/Histology
Spit for 5 minutes old school
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I am looking for a reliable method for staining mouse muscle with ATP'ase pH
4.3, 4.6 and 9.4.
Any help will be greatly appreciated.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent:
I would just like to thank everyone for all of your comments regarding the
high complexity testing issue. I obviously opened a can of worms on that
one! I think I understand it a little better now as I have come to the
following conclusion: Although CAP requires strict guidelines and
St. Anthony Hospital currently has an excellent opportunity for an experienced
Histologic Technician. This position requires Certification as an HT or HLT –
or – experience acceptable to the Laboratory Director. Two years of previous
histology experience required with IHC and/or grossing
I know I have asked about this in the past, but I'm finally getting
around to optimizing my antibody (Caspase3). First, tonsil will be a
good control?
Second, I ordered it from Abcam that is telling me the optimal dilution
is between 1/10 and 1/20. This seems a little crazy? I looked at some
Hello, my name is Caryn Cole, I am a recruiter with St Joseph's Hospital in
Atlanta GA and I have an opening for a Histology Technician at our Research
Institute. I have listed the job description and required qualifications about
the position below. If you are not interested please feel free
An Eerdekens overseas somewhere asks about thionin staining:
An, you may have a bad batch of thionin stain. Did you just open a new jar?
Here is the USA we use stains certified for use in histology by the Biological
Stain Commission. Each lot has been tested by them for use in histology.
So back at an old job we had an embedding station that had forceps that
plugged in and were constantly hot. Does anyone know where I can just
get the forceps that are always hot? I have the wells in my embedding
center, but it gets frustrating when embedding multiple tiny mouse
tissues and your
Hi Claire and all:
I think you are confusing this position with a different position in Madison.
The supervisor in this position will oversee Histology including IHC and the
gross room. There are no autopsies and no renal lab. The ideal candidate will
have experience with LEAN or workflow
Hello,
We started having a strange problem with our Ventana Benchmark XT. Out of the
blue, our slides stopped staining. With most antibodies we get no reaction,
some that were very strongly positive are now only very lightly staining. They
do appear to counterstain OK. Following Ventana's
Hi Mike,
Did you wash the bottles and rinse them and then pump the water through the
instrument before adding reagents and purging all again? We had a problem
like this about 2 weeks ago. I think someone loaded the wrong bulk reagent
onto the instrument. Cleaning it, purging with water and
Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md
From:
https://www.thermoscientific.com/wps/portal/ts/products/detail?navigationId=L10831categoryId=81937productId=12706060
http://www.medite.de/ebp50.html?L=1
Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen
A For filing reasons. When we file in the boxes on top of microtome they are
right side up and for final filing in the plastic drawers writing is right side
up.
Dawn
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
I think you can get from Thermo Scientific. Two sites found on line..j
http://www.medite.de/ebp50.html?L=1
https://www.thermoscientific.com/wps/portal/ts/products/detail?navigationId=L10831categoryId=81937productId=12706060
-Original Message-
From:
B
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu
-Original Message-
From:
I would highly recommend the Vantage system. We have had it in place for over
2 years and our accuracy rate went from about 94-94% to over 99.9%. We have
had only 2 numbering errors in Histo in over 2 years.(due to people not
following procedure correctly) The system does so much more that
I think they must stand on their head backwardsj:)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias
Sent: Wednesday, February 09, 2011 12:27
To: histonet@lists.utsouthwestern.edu
Subject:
Using the B method ends up with the case # right side up in the block
fileunless I completely misunderstood the orientations of A and
B; entirely possible!
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
Growing private lab searching for the following openings:
IHC Specialist (am)
Lead Histotech (8-4 T-S)
Histotechs for am T-S 3-11 M-f
Excellent pay - the highest out of any company we work with in the tri-state
area!
Part -time openings also available.
Please
Claire and I were thinking this was an advertisement for the University
of Wisconsin Hospital and Clinics Surgical Pathology manager position.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
We've used both systems here. Both can produce right side up filing,
depending on which way the wax faces (i.e. towards you, or away from you).
Because we hand label the cassettes here, I prefer to use A, because the
label bevel is at the top and easier to hold that way.
Jenee S. Odani,
Is anyone using the above CPT code for reviewing old pathology slides prior
to ordering IHC testing or can it only be used for slide review prior to
ordering molecular testing? Thanks.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection
Hello all-
We're seeking two experienced travel temps...both situations are day shift, for
up to 8 weeks. Please send resume for return call-
ad...@fullstaff.org
fax 800.756.3309
Permanent positions also available (perm, temp or temp-to-perm depending on
related experience and a few other
I believe the 25 cases are for ER/PR/Her-2 testing. We have done this recently
and we ran about 10 for each antibody. We have over 100 ourselves and it took
quite a while. There will be some antibodies that you will be unable to find
10 slides to test, so do as many (or as few) as your
Right. It is NOT with the University of Wisconsin.
On Feb 9, 2011, at 1:11 PM, Sebree Linda A wrote:
Claire and I were thinking this was an advertisement for the University
of Wisconsin Hospital and Clinics Surgical Pathology manager position.
Linda A. Sebree
University of Wisconsin
Only molecular... And I read that somewhere, but don't remember where. I'll
search. j
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Wednesday, February 09, 2011 13:12
To: Histonet
This can only be used for slide review prior to ordering molecular
testing.
Debra Lake MT(ASCP)
Manager Micro, Blood Bank, Pathology
Marion General Hospital
Marion, IN 46952
(765) 662-4648
If you are not the intended recipient(s), you are notified that any disclosure,
I just ordered one from VWR. It's for a thermo tome which I think will
fit on microms? It was $1600. I would call a tech help and see what
they would suggest.
Another thought would be to call IMEB (specifically Denise deVines
18005438496). I have had lots of good luck with the company and
In Tissue Tek block cabinets, front (smallest number) to back (larger numbers)
with numbers facing right side up as you pull out the drawer. Does that make
sense?
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC 29406
843-847-4586
Hello all,
I am trying to get a feel for how other core histology facilities are
charging. My main question is if or when technician's labor time is charged.
Currently we charge a fee per sample for embedding, whether it is paraffin,
plastic, or oct. Then we charge a fee per slide/grid for
We recently switched vendors for our formalin and while we have not experienced
any difference with our surgical specimens, our cell blocks from body fluids
have been giving us a great deal of trouble. The button that we get never
seems to harden, leaving it sort of gelatinous, even if left to
Sorry gang: the position in Madison wasn't the one I was thinking of. Disregard
warning. It is the position for the surgical path manager at UW Hospital in
Madison I was talking about.
Claire
I think you are confusing this position with a different position
Joe:
This is what I would do;
1- run 1 control slide per antibody you have in your arsenal
2- compare the result with a control slide already in your files.
3- show both slides to the chief pathologist (after all is his/her opinion the
one is going to be asked by CAP)
4- those antibodies whose
If when you finished the procedure the signal was strong, it is not a problem
with the antibody, it would have reacted with your working concentration, or
would not have worked.
I am inclined to think that the nickel solution you use to add to the DAB may
have deteriorated in that batch and
You CAN NOT use water to dissolve the diastase. You have to use diastase buffer
pH7 otherwise it will not work (as in your case).
René J.
--- On Wed, 2/9/11, Erin Sarricks esarri...@gmail.com wrote:
From: Erin Sarricks esarri...@gmail.com
Subject: [Histonet] PAS with Diastase Digestion
To:
It is my understanding that it used for examaination and selection of retrieved
archival (ie,previously diagnosed) tissue for molecular analysis (eg, KRAS
mutational analysis).
page 440 of the 2011 AMA CPT , professional edition.
Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory
What a sensible process! I agree with your approach.
Martha Ward, MT (ASCP) QIHC
Assistant Manager
Molecular Diagnostics Lab
Dept. of Pathology
Wake Forest University Baptist Medical Center
Winston-Salem, NC 27157
336-716-2104
-Original Message-
From:
As defined at the AMA site, you have a valid use of the CPT code 88363 to
claim for a review of old, previously diagnosed pathology slides prior to
ordering IHC testing. If reviewing for previously DX'd slides is not
enough, IHC is tagging the molecules in the cells at the binding site.
AMA-
Hi all
I am preparing to take the QIHC exam. Does anyone have any suggestions on
what I should study? What areas does the exam concentrate on and what
research material I can use.
Thanks
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Hi-
A flat fee per slide was charged which included all *technical* preparation
for the final slide. Also prior to the start of a project a cost estimate
was compiled and submitted for review/approval. As portions of projects
were completed we submit via the grant or project number the work
Dear Nele,
I hear of these problems very often. It would be worth your time looking for
any information on fresh frozen bone sections by Dr. Dodds, there should be a
number of papers he has published on good quality bone sectioning without
the use of the slow, expensive and troublesome tape
I would also see if Ventana can send you some test slides to test the
heating. At my old lab, we had a problem with the heating plates. They
repaired the entire platform. Just a thought.
Joe
- Original Message -
From: Mark Tarango marktara...@gmail.com
To: Dessoye, Michael J
Sakura also has the heated forceps that plug directly into the embedding
center.
Weems, Joyce jwe...@sjha.org
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/09/2011 09:23 AM
To
sgoe...@mirnarx.com sgoe...@mirnarx.com,
histonet@lists.utsouthwestern.edu
You Wrote (two messages)
Dear Nele,
I hear of these problems very often. It would be worth your time looking for
any information on fresh frozen bone sections by Dr. Dodds, there should be
a number of papers he has published on good quality bone sectioning
without the use of the slow,
Hello helfpul Histonetters!
I am curious about your formalin-fixed paraffin embedded (FFPE) tissue
protocols. If the tissue has been fixed with saline and paraformaldehyde during
perfusion, then further fixed in paraformaldehyde for 24 hours, do you still
utilise a sucrose wash before running
Dear Colleagues
Please remove my husband, Charles E Brown Jr. from you listings. My husband
passed away on 1/27/11.
Respectfully
Mrs. Charles E Brown, Jr.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Dear Histonet Colleagues
My husband, Charles E Brown Jr, passed away 1/27/11
Respectfully,
Mrs. Charles E Brown Jr.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Wednesday,
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