RE: [Histonet] Information Needed

2011-02-18 Thread Liz Chlipala
Pamela We use a hepa filtered vacuum from Marmed, Inc. (440) 572-5175 LiZ Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Lon

[Histonet] Job Opening

2011-02-18 Thread Michael Hillmer
We are looking for a certified HT or HTL to join our pathology lab in Manitowoc, WI. We have a currently 2nd shift position open. We have excellent benefits, state-of-the-are lab (less than 3 months old) and a great team to work with. We will assist with relocation. Please contact me if you hav

[Histonet] LKB Bromma 7800 knifemaker

2011-02-18 Thread kgrobert
Yes, I know this is ancient. But does anyone know of a service company that can repair this machine? Ours works, sort of, but it's very frustrating. Thanks and have a good weekend, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmaco

[Histonet] Job Descriptions

2011-02-18 Thread Cristi stephenson
Hello Histoland, I was wondering if anyone out there may have their job decriptions for histologists, lab assistants, supervisor and/or medical director available and would be willing to share.  I am currently revamping mine and am struggling with some of the verbiage.  Thank you all and have

[Histonet] Information Needed

2011-02-18 Thread Marcum, Pamela A
Hi, I need information for purchasing a good vacuum with proper biological filtration for cleaning a cryostat. We are not buying a new cryostat and I do know of several with a decontamination feature, it is not in the budget for the next two years. Thanks, Pamela A Marcum Anatomic Pathology

RE: [Histonet] Re: IHC pretreatment with NaOH/H2O2

2011-02-18 Thread Montina Van Meter
Neil, When I read the paper in question, it reminded me of the pretreatments one would use in an In Situ Hybridization protocol. I have used this protocol in the past and it doesn't seem to harm the tissue as long as the tissue has been adequately perfused. We use the pSTAT3 #9131 (Cell Signaling

[Histonet] Re: IHC pretreatment with NaOH/H2O2

2011-02-18 Thread Johnson, Teri
Dear Neil, I was able to find a protocol like you described published where they had done a regular IHC protocol on free-floating tissue sections for a-MSH antibody using 0.3% H2O2, normal serum block, etc. They go on to describe that for P-STAT3, "the tissue needed to be pretreated with 1% NaO

[Histonet] RE: Histonet Digest, Vol 87, Issue 33

2011-02-18 Thread Goodwin, Diana
We are also fans of the Leica ASP 300 processor. Diana G. Goodwin, BS, HT(ASCP)QIHC Department of Pathology Robert Wood Johnson University Hospital at Hamilton Hamilton, NJ 08610 609-631-6996 dgood...@rwjuhh.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto

[Histonet] Microwave Usage, Monitoring procedure

2011-02-18 Thread Scott, Allison D
Hello to all in histoland. I am in need of a procedure for the usgae, monitoring and container venting of the mcrowave. I have a procedure that I wrote several years ago, but my boss wants it updated. Any help will be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston

RE: [Histonet] Tissue Processors

2011-02-18 Thread sgoebel
Pathcenters in my opinion don't last as long? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utso

[Histonet] Histotechnologist with a FL HTL license for Fort Myers, FL

2011-02-18 Thread Brian- Prometheus
Looking for a Histotechnologist with a FL HTL license for Fort Myers, FL. This will be a late second or third shift. Experience with IHC and Ventanna is preferred. Please contact me today for immediate consideration. Brian Feldman Principal Prometheus Healthcare Office 301-693-9057

Re: [Histonet] Eosinophilic new bone formation

2011-02-18 Thread Jack Ratliff
In decalcified sections of bone, yes the osteoid or dense unmineralized collagen matrix (mostly type I) will stain darker than mature native demineralized bone. Even though the mature bone has been demineralized, it is still more densely compact as compared to the newly formed bone that has not

RE: [Histonet] Tissue Processors

2011-02-18 Thread Houston, Ronald
I would probably have gone along with you on this Rene but since we demo'ed, and subsequently purchased, the Peloris my opinion has changed Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@l

RE: [Histonet] Tissue Processors

2011-02-18 Thread Nails, Felton
Joe, I just purchased a VIP6 because of my experience with this brand both while in the military and civilian life. Have never had a problem with them. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe N

Re: [Histonet] Tissue Processors

2011-02-18 Thread Rene J Buesa
Sahura is still the best buy (for me). René J. --- On Thu, 2/17/11, Joe Nocito wrote: From: Joe Nocito Subject: [Histonet] Tissue Processors To: "Histonet" Date: Thursday, February 17, 2011, 8:55 PM Greetings all, if you had to purchase new tissue processors, which one would you choose? Mi

[Histonet] Eosinophilic new bone formation

2011-02-18 Thread Keller, Pat
We have always noticed, at least in the middle/inner ear, that newly deposited bone stains darker than mature bone, both with H&E and toluidine blue. Is this increased eosinophilic quality due to a lack of mineralization and therefore higher density of osteoid components in the new bone or some ot