Re: [Histonet] Poor nuclear artifact

2011-02-25 Thread Rene J Buesa
My problem in understanding your difficulty is the definition: what is "poor nuclear artifact"? No detail? Weak staining? Strong staining? "Empty" nuclus?  The cause of the problem will vary depending on its definition. Unless you are more specific I think it will be very difficult in trying to h

[Histonet] Poor nuclear artifact

2011-02-25 Thread Marshall, Kimberly K
Happy Friday Histo people!!! I need some opinions on a processing issue I cant seem to fix. I run a short processing run each night for my small biopsies. With two processors, I rotate so the machine with the newest reagents is the one we run as the biopsy machine. For several weeks now my

Re: [Histonet] Checks and balances for specimen accessioning

2011-02-25 Thread Victoria Baker
Do you recall the source of pick up for the specimen? If it is picked up by Histology either in the OR or a lab site the pick up person should be checking the name on requisition, container and label in the book or ledger prior to initialing and dating. Also the person bringing the specimen to th

RE: [Histonet] cdj

2011-02-25 Thread Horn, Hazel V
This is what I found at the CDC website: "Table 9. Tissue Preparation for Human CJD and Related Diseases 1. Histology technicians wear gloves, apron, laboratory coat, and face protection. 2. Adequate fixation of small tissue samples (e.g., biopsies) from a patient with suspected prion disease ca

RE: [Histonet] Checks and balances for specimen accessioning

2011-02-25 Thread Blazek, Linda
That's my goal in the next year! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwes

RE: [Histonet] Checks and balances for specimen accessioning

2011-02-25 Thread Mike Pence
But you have to hope the whom ever is putting the barcode label on uses the correct patient barcode. That has been the problem that I have seen. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Miha

RE: [Histonet] Checks and balances for specimen accessioning

2011-02-25 Thread Michael Mihalik
One quick clarification: If the OR or whomever collects the specimen would put a barcoded label on the specimen container at collection time, all of this would be avoided. That just seems to be a difficult thing for those departments to do. I know that it's not a technical issue. It's something

RE: [Histonet] Checks and balances for specimen accessioning

2011-02-25 Thread Mahoney,Janice A
Same here Sue, thanks for the reply. Jan -Original Message- From: Finley, Sue [PH] [mailto:sfin...@providencehealth.bc.ca] Sent: Friday, February 25, 2011 12:08 PM To: 'Michael Mihalik'; Mahoney,Janice A; 'Nita Searcy'; 'Allison D' 'Scott'; histonet@lists.utsouthwestern.edu Subject: RE: [

RE: [Histonet] Checks and balances for specimen accessioning

2011-02-25 Thread Finley, Sue [PH]
Hi All We too receive samples from our OR clinics and wards, referred-in etc. The example that you have illustrated in this e-mail thread is a very common occurrence and as Michael states bar coding cannot control the initial point of entry into the lab. It is the responsibility of the person

RE: [Histonet] Checks and balances for specimen accessioning

2011-02-25 Thread Michael Mihalik
..but Janice, Vantage won't help with accessioning issues will it? I'm just going back to this issue because that was the initial point of the thread and it's really, really important to get things right at accessioning. The other aspect of this is that accessioning is the point at which materi

[Histonet] kits for LCM & archival FFPE tissue

2011-02-25 Thread Margaryan, Naira
Hi all, I am about to perform PCR on archival FFPE tissue after LCM. My LCM system is from Arcturus and my tissue is on membraned glass slides. My questions are: 1. What Kit to use to get the highest yields and quality RNA? 2. Can anyone who has been doing it for the past 2-3 years suggest m

RE: [Histonet] Checks and balances for specimen accessioning

2011-02-25 Thread Mahoney,Janice A
Allison and all, As part of LEAN we use standard work. This means we have best practice written down, step by step and every person does it the same way on every specimen. No matter what! This gets "hard-wired" after a while. The inspection of the requisition against the container is one of th

Re: [Histonet] Thanks

2011-02-25 Thread Paula Pierce
Texas Rocks! 2 years and 2 months until I'm home.   Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: "sgoe...@mirnarx.com" To: cp...@x-celllab.

Re: [Histonet] Thanks

2011-02-25 Thread Mark Tarango
So cruel! On Fri, Feb 25, 2011 at 8:13 AM, wrote: > It's sunshine and 75 here in Texas...nanny nanny...I do love my state's > weather!! We had shorts on yesterday =) > > Sarah Goebel, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744

RE: [Histonet] Checks and balances for specimen accessioning

2011-02-25 Thread Nita Searcy
You are absolutely correct. Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 >>> "Michael Mihalik" 2/25/2011 10:22 AM >>> To me, there is only one 100% solution -

Re: [Histonet] Telepathology

2011-02-25 Thread Patrick Laurie
I believe that Telepathology should be the name of what one of our pathologists wanted us to do. "Cut to the small focus of tumor and stop". On Fri, Feb 25, 2011 at 8:12 AM, wrote: > It's not nice to make fun of people's typing boo-boo's, but thanks for the > Friday chuckle!! > > Sarah Goebel,

RE: [Histonet] Checks and balances for specimen accessioning

2011-02-25 Thread Michael Mihalik
To me, there is only one 100% solution -- barcodes. I'm quite anxious to hear other people's thoughts, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369       -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histon

RE: [Histonet] Thanks

2011-02-25 Thread sgoebel
It's sunshine and 75 here in Texas...nanny nanny...I do love my state's weather!! We had shorts on yesterday =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-b

RE: [Histonet] Telepathology

2011-02-25 Thread sgoebel
It's not nice to make fun of people's typing boo-boo's, but thanks for the Friday chuckle!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouth

[Histonet] Checks and balances for specimen accessioning

2011-02-25 Thread Scott, Allison D
Hello to all in histoland. What types of checks and balances do you have in place for specimen accessioning. We had a incidcent where I was accessioning a case and I did not catch that the name on the container was different from the name on the requisition. The resident grossing did not catch i

Re: [Histonet] IHC Equipment

2011-02-25 Thread Victoria Baker
Sheila It is impossible to always get everything into an e-mail when you recieve a general info request. I thought what you said was great. Have a wonderful weekend. Vikki On Fri, Feb 25, 2011 at 10:25 AM, Sheila Fonner wrote: > Vikki, > > > > You are absolutely right! We do all of our ISH

Re: [Histonet] Telepathology

2011-02-25 Thread Emily Sours
Okay, it's Friday so I have to write that my first thought was telepathology? Is that like telekinesis? Like processing slides and coverslipping with your mind? Would this extend to sectioning to? How about pouring your much needed cup of coffee, or even making the coffee? Emily It has become alm

[Histonet] Thanks

2011-02-25 Thread Cynthia Pyse
Happy Friday Histonetters I want to thank everyone for the information on IHC strainers. This is just what I needed. People who actually use the machines on a daily basis giving their opinions. What a great forum we have. Hope everyone has a great weekend. Currently we are receiving 6-10 inches of

[Histonet] Deadline extended

2011-02-25 Thread Shirley A. Powell
Hi Everyone, Good news, the hotel extended the deadline for the GSH discount rate. As long as they have rooms they will honor the $99 @ night rate that includes continental breakfast and entrance to the park. There is no deadline for registering for the meeting, but for us to have name tags re

RE: [Histonet] IHC Equipment

2011-02-25 Thread Sheila Fonner
Vikki, You are absolutely right! We do all of our ISH as overnight runs to avoid the problem of space constraint with the detection kits, etc. I agree that all of the instruments have their good and bad points, and it really depends on the lab and what kind of specimens/volume you have. D

RE: [Histonet] procedural safeguards when accessioning identical sources

2011-02-25 Thread Cynthia Pyse
Carol We mark all our prostates with dye for ease when embedding. For prostates that need to be accessioned in order we alternate the color of the dyes, hematoxylin, safranin, eosin. When grossing, the color of the dye used is in the dictation. Hope this helps. Cindy Cindy Pyse, CLT, HT (ASCP) Lab

[Histonet] Telepathology

2011-02-25 Thread Andrew Byrnes
Dear Histonet, Thank you for your responses to my email this past week! If you have any additional thoughts about telepathology or digital pathology, please email me or call me directly. Have a nice weekend! Andrew Andrew Byrnes VP Sales and Marketing AccelPath, LLC M: 732-312-8008 www.A

RE: [Histonet] IHC Equipment

2011-02-25 Thread Sheila Fonner
Sorry if I confused anyone. It may have been FISH that we couldn't do on the Leica instrument. There was some reason that the docs chose to go with the Ventana. Anyway, I'm sure they are both fine instruments! Sheila From: Greg Dobbin [mailto:gvdob...@ihis.org] Sent: Friday, February 25

Re: [Histonet] IHC Equipment

2011-02-25 Thread Victoria Baker
In regards to the Ventana Ultra - if you are running PIN-4 or ISH simultaneously on this instrument, the kits/ab's/probes they require take up a lot of space on the reagent carousel which limits the number of antibodies you can put on and as runs can go some where around 6-hours you could find you

Re: [Histonet] cdj

2011-02-25 Thread Pamela Marcum
The handling of CJD and other prion diseases is on the CDC website and that is the best place to understand the issues with equipment usage and contamination as well as procedures.  Many people have made comments however; the true issue is not just how to handle it but what you can not use aga

Re: [Histonet] cdj

2011-02-25 Thread Emily Sours
Now I'm curious!! Please let us know what this mysterious processing is!! Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On

[Histonet] Diff quik and rapid H&E billing for touch preps

2011-02-25 Thread Konop, Nicole
Happy Friday! I am wondering if anyone can give me feedback in regards to touch prep billing when using diff quik and rapid H&E staining. Our facility uses both during frozen section. I am wondering if I can bill 88333 twice per part (one for rapid H&E, one for Diff quik) and then additional

RE: [Histonet] IHC Equipment

2011-02-25 Thread Greg Dobbin
The Bond does do ISH. In fact it utilizes the same detection kit so you have only to buy the probes, not the additional detection kit that could (depending on your volume of ISH requests) expire. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen E

RE: [Histonet] IHC Equipment

2011-02-25 Thread Sheila Fonner
Cindy, We used to use the Dako stainer, and we still have it as a back-up if necessary, but we have recently (in the last year) bought a Ventana Ultra. You can put 30 slides at a time on it, but you do not have to batch the slides. It is a continuous feed machine, which means that as soon as a sl

[Histonet] procedure guidelines

2011-02-25 Thread Hutton, Allison
We are in the process of revamping our procedures to be in the same format throughout the entire laboratory. I have been asked to find out if there is any format guidelines particularly for histology. We are using the CLSI standards but histo and cyto are finding that we don't quite "fit the m

RE: [Histonet] Question from LIS vendor

2011-02-25 Thread Rathborne, Toni
Yes. After the final report is signed out, any changes, additions, or corrections have to be put through as an addendum. This becomes part of the patient's permanent record. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.

[Histonet] leaking specimens

2011-02-25 Thread Stacy McLaughlin
Happy Friday Histonet! I'm wondering how folks out there deal with specimen containers that are leaking when received into the lab. Do you inform the physician's office, send it back, not inform anyone, etc? Thanks! Stacy McLaughlin, HT(ASCP) __

RE: [Histonet] procedural safeguards when accessioning identical sources

2011-02-25 Thread Horn, Hazel V
We use different colored cassettes. We have 6 different colors and rotate them with the cases. The color of the cassette is also dictated as part of the gross description. We use the same color of slides as the cassettes to carry this one step further. Hazel Horn Hazel Horn, HT/HTL (ASCP) Su