Aureus Medical Staffing - Omaha NE
Club Staffing - FL
TravelMax - not sure where they are located
Ampian - Nevada
Full Staff - TX
CompHealth - not sure which site does travel work at this point
These are just a few of the many I'm sure.
Vikki
On Wed, Mar 2, 2011 at 12:37 AM, Jennifer MacDonald
Has anyone experience of cryostat sectioning of fat tissue? This would
be rodent fat pads, particularly the gonadal fat pad which usually
consists largely of adipocytes.
What temperature would you cut at? Is there anything that could be used
to infiltrate the tissue prior to sectioning to
Allison Scott HT(ASCP), Histology Supervisor at LBJ Hospital in
Houston, Texas asks about sentinel lymph node procedures.
About a month ago an article appeared in the New England Journal of
Medicine suggesting that a single section of a lymph node block,
without immunostains, was sufficient for
Yes, it is always best to run every conceivable control available. Then you can
be really, really, really sure. However, if you have practical issues that come
into play such as cost of reagents, or extra controls taking up valuable space
on the stainer then you might have to think about what
For the most part you cannot section fat on a cryostat because of the
oilyness (is that a word). You can fix it and section it?
Good Luck
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-Original
The CAP guidelines are pretty clear. Copied from latest checklist.. Isn't this
fun???! j:)
ANP.22570 Phase IIN/A YES NO
Are appropriate negative controls used?
NOTE: Negative controls must assess the presence of nonspecific staining in
patient tissue as
Thanks Joyce. This excerpt supports what I had said- noting the
difference between the negative (or deletion) control (which is what I
was addressing in my reply) and the negative tissue control.
Cheers!
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Hi All,
I would appreciate some insight as to how most labs are treating bone marrow
smears, in regards to fixation. I am not embarrassed to say that it has been
awhile since I worked with them. With the smears are most labs air drying,
methanol (alcohol fixation) or spray fixation (pap
Either air dry → methanol (most labs) or air dry → PAP fixative (I always
preferred methanol).
René J.
--- On Wed, 3/2/11, Debra Siena dsi...@statlab.com wrote:
From: Debra Siena dsi...@statlab.com
Subject: [Histonet] bone marrow aspirations
To: histonet@lists.utsouthwestern.edu
you all saw the little wink ;) at the end of his paragraph, right
Shirley A. Powell powell...@mercer.edu 3/1/2011 3:01 PM
Bet you he already does.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
I've generally used the methanol fixation.
Mark Turner
Rene J Buesa rjbu...@yahoo.com wrote:
Either air dry → methanol (most labs) or air dry → PAP fixative (I always
preferred methanol).
René J.
--- On Wed, 3/2/11, Debra Siena dsi...@statlab.com wrote:
From: Debra Siena
Fun no, impractical yes.
Weems, Joyce jwe...@sjha.org 3/2/2011 10:30 AM
The CAP guidelines are pretty clear. Copied from latest checklist..
Isn't this fun???! j:)
ANP.22570 Phase IIN/A YES NO
Are appropriate negative controls used?
NOTE: Negative controls
We always air dried them and fixed them in methanol, otherwise they washed
off.
Debra Siena dsi...@statlab.com
Sent by: histonet-boun...@lists.utsouthwestern.edu
03/02/2011 07:49 AM
To
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
cc
Subject
[Histonet] bone marrow
For breast sentinels:
Sentinels are received fresh.
Sentinels below 4 mm diameter are cut in two halfes - one for frozens, one
for paraffin.
Sentinels bigger than 4 mm diameter are cut in 2 mm slices - the
middle-slice for frozens, the rest for paraffin.
Frozens: 3 sections; 1. and 3. for HE;
Hi All,
It appears that air drying and then methanol fixation is the method most labs
are using on bone marrow smears. Thanks for all your help, I do appreciate all
the responses. Best Wishes
Debbie Siena HT(ASCP)QIHC
Technical Manager | StatLab Medical Products
407 Interchange St. |
Could I PLEASE have this information, too? I would really appreciate it.
Joyce Fortin
Histology Supervisor
Palmdale Regional Medical Center
38600 Medical Center Drive
Palmdale, California 93551
Phone 661-382-5723
Fax 661-382-5747
email: joyce.for...@uhsinc.com
Hi Everyone in histoland!
I would like to get your feedback on which alcohol / xylene recyling units you
prefer. I would like information regarding purity of end product, cost, size
of
footprint, and relyability. We currently do not have a recycling unit, and I
have been requested to gather
So...I have run down slides and done antigen retrieval on my FFPE
slides. They are currently in antigen retrieval that has come to room
temperature. I am not going to be able to finish the IHC stains until
tomorrow. Will it be better to keep the slides in the antigen retrieval
solution at 4
We've held slides post-retrieval in buffer (tris, PBS, etc.) overnight
with no problem.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From:
You can put them %3 H2O2 for 10 minutes. and then drop primary antibody and
incubate at 4 degrees overnight.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
one of the largest, most sophisticated, state-of-the-art laboratories in the
Northeast is looking for:
both a histology supervisor and a histotech for the 3rd shift with a
differential of $3.50 per hour
Both positions are eligible for a sign-on bonus (up to 5k) and relocation
assistance
Just hold them in buffer overnight.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@mirnarx.com
Sent: Wednesday, March 02, 2011 1:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC
Janice Mahoney is retiring from this job and thought you might find it
interesting.
Coordinator Laboratory Histology Cytology FT
Alegent Health System
System/Corporate
You can view and apply for this job at:
In buffer at 4ºC overnight
René J.
--- On Wed, 3/2/11, sgoe...@mirnarx.com sgoe...@mirnarx.com wrote:
From: sgoe...@mirnarx.com sgoe...@mirnarx.com
Subject: [Histonet] IHC slides
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, March 2, 2011, 2:24 PM
So...I have run down slides and done
I have been asked to stain mouse hearts for cardiac myosin. I have tried the
antibody from Abcam (ab50967-Mouse monoclonal to heavy chain cardiac myosin)
with no luck. I am using the Mouse on Mouse kit from Biocare and it worked well
for me for some other mouse antibodies on mouse tissue but
Mark
I took a brief look at the abcam antibody and in particular the review
with the paraffin staining. If I was purchasing the antibody I would
question the results of that review, since they used a goat anti-mouse
detection system which is not ideal for mouse tissue. Plus I'm not sure
that
We attempted to cut a knee without decalfication. We saw that we can do
that with technovit 9100.
This was our protocol:
- Fixation with 4% formalin 24h room temp
- Ethanol 50% 1h room temp
- Ethanol 70% 1 h room temp
- Ethanol 80% 1h room temp
-
We attempted to cut a knee without decalfication. We saw that we can do
that with technovit 9100.
This was our protocol:
- Fixation with 4% formalin 24h room temp
- Ethanol 50% 1h room temp
- Ethanol 70% 1 h room temp
- Ethanol 80% 1h room temp
-
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