[Histonet] EGFP expression in mouse testis cryosections

dear all, i am relatively new to histochemistry. i would like to visualize EGFP expression mouse testis. i am not sure if this is expressed in cytoplasm or nucleus. what kind of staining i should follow and are there some protocols that can be of help for me. please let me know if i need to

[Histonet] Re: NSH - One Day VIR Forum VIR Teleconference Series

I have been promoting this NSH program in my last three shows! Well worth signing up and attending. But, then again all of the NSH conferences are worth attending. :- ) David Kemler, SLS, HT(ASCP)HTL Lab Manager, Gary L. Heller Dermatology Laboratory and Host of www.HistoTALK.com

[Histonet] (no subject)

There is a Pathologist Assistant I know who is looking for a position in the Baltimore/Maryland area. If anyone knows of any Pathologist Assistant opportunities in this area, please contact me at wilderpa...@hotmail.com Thank you so much!

RE: [Histonet] EGFP expression in mouse testis cryosections

Hi Muralidhar, Fix your samples in 4% formaldehyde on PBS (you can prepare it from powder or from 20% solution in water sold by Electron Microscopy Sciences) about 4h at room temp, wash with PBS (at this step you can store your samples at +4 C for several days or even weeks, cryoprotect by

[Histonet] STAINING CONTAINER LIDS

We are looking for SAKURA Tissue Tek DRS stainer - container lids. ( This is the dual stainer). Does anyone know who have them. Thanks Nirmala Srishan Histology Supervisor Holy Name Medical Center. Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in

[Histonet] Alcian Blue-Acid mucosaccharides with and without hyaluronidase

Does anyone out there do this stain? Alcian Blue-Acid mucosaccharides with and without hyaluronidase? Where do you get the testicular hyaluronidase? Thank you so much. This for a student. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA

Re: [Histonet] Alcian Blue-Acid mucosaccharides with and without hyaluronidase

On 3/3/2011 1:47 PM, Kathy M. Gorham wrote: Does anyone out there do this stain? Alcian Blue-Acid mucosaccharides with and without hyaluronidase? Where do you get the testicular hyaluronidase? Thank you so much. This for a student. Kathy Gorham H.T. You can get the enzyme from Sigma. Note

[Histonet] SOX11 antibody

Hello all, I have been asked to try to work up the SOX11 antibody for a research project. The antibody they gave me is from Sigma Life Science. I am having difficulty getting any staining at all. If anyone has any suggestions I would be very grateful.We are using a Leica Bond 3.

RE: [Histonet] SOX11 antibody

Hi Martha, I haven't used it, but you can find some info on it from the human protein atlas here: http://www.proteinatlas.org/ENSG0176887/antibody They recommend 1:100 and you can check out Sigma's recommended procedure for using their Prestige antibodies here:

RE: [Histonet] Competancies for handling hazardous material

We did a self audit about three or four years ago. My area is considered a small waste storage area and yes there is required training. In New Hampshire, we can get that training through the state. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare

[Histonet] JOB POSITION PENNSYLVANIA

We have a full time position in a GI practice laboratory in Easton Pennsylvania.  Candidate must have 2 years experience.  Must be proficient in grossing and  have ASCP certification.  Position is Mon-Fri, flexiable hours with benefits.  Possibility of future supervisor position .  

[Histonet] Need to compare prices

Hi all, We do Histology and Immunology primarily on liver tissue and only for research. I need to check the prices of other cores and present numbers for comparison as soon as possible. I remember that some time ago there was a question about prices for Histology in a research

[Histonet] Processing Specimens by CPTs

Do CPTs (phlebotomist) do any type of specimen processing at your facility? Specifically, if a cytology specimen, for example, one liter of pleural is received in the lab and there are multiple orders for the specimen (cultures, cell count, perhaps a test that has to be sent out, etc), who

Re: [Histonet] negative controls on immunos

yes. I'm in the beginning stages of validating a new immuno machine. Because we have over 120 antibodies, I'm guessing about 2500 slides. Guess who pays for that testing? Certainly not CAP. I hate monopolies. Even though I know labs who dropped CAP and went with JCAHO, CAP is still a monopoly.