Classification: UNCLASSIFIED
Caveats: NONE
Dear Histonetters,
Our 11 year old cryostat is beginning to experience some unusual problems. Our
Medical Maintenance says that they won't put a lot of money into fixing it
because of the age of the unit. Therefore, I am asking for opinions, pros,
Hi Diane,
We have been happy with the Leica cryostats over the years. We have an 1800
that is about 15 years old, an 1850 that is about 5 years old with the UV light
for decontamination.
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL
I would make smears and a cell block(if enough specimen) and charge 88104 and
88305. Pap on the smear, HE on the cell block.
Jan
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Tuesday,
To all of you Histonetter's out there I need to know for those of you that are
using the new Ventana antibody, CK34BE12 + p63, Basal Cell Cocktail, are you
charging these as as one charge or two? Any information will be greatly
appreciated. Thanks.
Sharon Davis-Devine, CT (ASCP)
We also transport smears from the clinical lab to our Pathologist and transport
them back. The Hematology techs put the Pathologist's comments into our
computer system for review by the clinicians.
Wanda
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza
I will be out of the office starting 03/25/2011 and will not return until
04/04/2011.
I am on vacation until Monday, April 4th. Please contact David Archambault
if you need immediate assistance. Thanks!!!
__
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Breast Processing: Looking to set up a breast protocol on our Sakura VIP
processor. Looking for times/duration of specific solutions and types of
solutions used (ie. PEN-FIX).
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Sharon,
We're actually doing a triple stain of HMWCK + p63 + p504S but charging
as a single stain. I am advocating charging for at least 2 of the 3
stains (done in 2 separate runs) since one can easily differentiate
between 2 different chromogen colors. The jury's still out.
Linda A. Sebree
Gary Martin (where?) asks: Our pathologist[s] review peripheral
smears for the clinical lab, and I'm wondering how other facilities
handle the reporting. We presently receive the smear from a tech, the
pathologist provides a comment and returns it to the lab.
I take these VERY seriously. First,
To add to my earlier diatribe: I do not want a slide dripping with
immersion oil to mess up my microscope. I expect you to mount the
smear in resin. Many histotechnologists are uncomfortable doing this,
so I often coverslip the slide myself. I'll use immersion oil on the
coverslipped slide if I
Hello Histoland!
Does anyone know how to get in touch with Rita Humphrey, previously
with Childrens Hospital, Birmingham, AL? I lost touch with her after
she left Childrens and would like to re-connect.
Thanks in advance,
Akemi
Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab
Thank to everyone who responded to my request for information concerning
peripheral smear review. It seems that most respondents are doing this
the way we presently do. I have to say that I agree totally with Dr.
Richmond's method. I will be presenting the idea that these slides are
consults
We accession the peripheral smear into our Clinical Microscopy module of
the computer program. We stain the slide, coverslip it and give it to the
pathologist with a hematology CBC print out. The pathologist interprets
the slide and dictates the report. The transcriptionist types the report
Hi Histonetters!
I posted this the other day, but I guess it nested itself in the April Fools
email. So, I thought I would post it again under its own heading!
Can anyone tell me (maybe even provide a reg) as to whether or not a lab
aide can run the Histos 5 microwave tissue processor? It's
Hi Michelle,
This issue has been discussed at the clinical board meeting and it has been
said
that moving a carousel from one beaker to another is considered part of the
specimen processing steps, making it part of a licensed task. The histology
scope of practice includes as a licensed task
Dr. Sendelbach,
We had a microwave processor in the past, and switched back to a routine
shortened biopsy run for several reasons. It is possible that the technology
since then has improved to where you would not experience issues. However,
microwave processing usually carries additional
Hi Histonetters,
I have a quick question regarding Weigert's Iron Hematoxylin and Gill's
3 Hematoxylin. One of the guys here thinks that we can interchange these
2 stains. I am attempting to perform a Masson Trichrome stain and it
calls for Weigert's Iron Hematoxylin. This person in my lab
Iron hematoxylin is more resistant to decolorization than aluminum
hematoxylin. Since there are so many acid steps that follow the
hematoxylin, it is wise to use the Weigert's so you do not have to worry
about decolorizing the other tissue elements too far. However, if you
optimize your stain to
Sharon,
I do a CK34BE12 P63 cocktail for basal cells charge for only one stain.
That being said, you can absolutely charge for 2 as P63 in nuclear the
CK34BE12 is cytoplasmic, so the pathologist can discern one from the other. I
am considering setting it up as a double charge in the
Hi Kimberly,
Imho because of the color, Weigert's Iron Hematoxylin is dark brown. Gill's
3 is blue. anilin blue is also blue. imho it would be better stain with
Weigert's Iron Hematoxylin.
On Wed, Mar 30, 2011 at 10:55 PM, Blundon, Kimberly
kimberly.blun...@drdc-rddc.gc.ca wrote:
Hi
In our lab, the hematology dept has a form that they present to the pathologist
with a manual differential( if only an automated one was done originally) and
CBC with abnormal results , and a list of check boxes indicating whether they
agree with technologist interp or if a written consult is
Hi,
Could anyone suggest a good anti-mouse CD3?
I am looking at staining aortic root frozen sections fixed with formalin.
Thanks!
Delphine
Delphine Eberlé PhD
UCSF Department of Vascular Surgery
VA Medical Center - NCIRE
Building 2 - room 410
4150 Clement Street
San Francisco, CA 94121,
Hi Diane,
We just purchased a cryostat from Sakura, we love it. Great features.
Date: Wed, 30 Mar 2011 06:38:41 -0700
From: dch...@yahoo.com
To: diane.glad...@us.army.mil; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)
CC:
Hi Diane,
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