We had the same problem years ago using Clear-rite. It turned out to be
the mounting medium was not compatible. We use Clear-rite routinely and
do not have problems with the eosin bleeding. We use Permount or on our
coverslipper we use Sakura brand.
Jennifer
Sent by: histonet-boun...@li
Hello John,
Just reading through some histonet archives and found your Elastic- Masson
issue.
Some notes on the elastic masson stain The fibers should darken when the
slides go into running water after the 2% ferric chloride. So the color before
the ferric chloride will not be the same inte
Oh yes, how to fix it.
Hydrate through clearite, 100% ETOH, 95% ETOH, and water. Spend an extra 5
mins in running tap water -- that should wash out most of the eosin. Then
counterstain again with eosin.
Dehydrate with the clean ETOHs and Clearite.
That should do it.
Another possibility I jus
Sounds like your dehydrating alcohol is not dehydrating completely. I would
switch out the 100%'s and the clearite. Either they got "wet" during the course
of the day or someone made an oops when changing the stain line.
Hope that helps.
Will
Sent from my iPhone
On Apr 7, 2011, at 2:06 PM,
So I just stained a group of slides all at the same time with the same
conditions. About 40 of 150 the eosin looks like it is bleeding out of
the sections...this has never happened before? What could be the cause
and how do I fix it? Everything is as normal, but again I am being
forced to use th
What LIS are you using? This sounds great.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Jesus
Ellin
Sent: Thursday, April 07, 2011 2:32 PM
To: Victor Tobias; Webb, Dorothy L
Cc: histonet@lists.utsouthweste
We currently use our LIS to support this issue,, the tech are able to go
into the case and make comments about controls, staining, technical work
etc. There are places for comment section, etc. I also use the LIS to
document Fixation time for specimens. Multiple specimens are an issue,
but we are
Thanks all who responded. Problem solved.
At 7:45 AM -0500 4/3/11, Bill B. wrote:
>I lost my histotech this weekend and need an immediate full time replacement
>for basic surgical pathology histology and low volume frozen sections.
>
>Our lab is near Cape Girardeau.
>
>Contact
>--
>
Dorothy,
I'm only going to address what we are doing for IHC. We have a custom
worksheet that is printed from the LIS for each patient. We were totally
manual until 3 weeks ago when we went live with the Bond. We are still
using our custom worksheet for the techs and pathologists. Boxes for
Does anyone know of a good antibody to detect rabbit heterophils (neutrophils)?
I'd love one that works for FFPE, but we can go frozen if necessary.
Thanks in advance!
Beth Villarreal
Preclinical Safety, Discovery Pathology
Novartis Institutes for BioMedicalResearch, Inc.
300 Technology Squar
How does everyone handle their QC documentation on special stains and IHC? We
currently print out the run information from our stainer(s) and have the tech
initial for her QC and the pathologist sign after they review the slides. I am
hoping that someone has a way of doing this electronically.
Hi please remove me from the mailing list
-- Original --
From: "Vanessa Avalos";
Date: Thu, Apr 7, 2011 05:54 AM
To: "HISTONET LISTS";
Subject: RE: [Histonet] Slides sent out for outside consult.
Our practice does not bill the patient. Before sendin
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