Christina,
The following might be useful:
Kappel et al (HUM PATHOL 27:1361--1364, 1996) attempted to grow TB from
formalin fixed lung tissue that had previously been shown to be positive by
sputum culture. They were unable to culture TB from these tissues.
Gerston et al (HUM PATHOL
Hello,
We do have a successful way to transfer tissue sections. It is using Mount
Quik liquid coverslip. We purchase this from Newcomber Supply. Are you doing
IHC testing?
1. HE sections first (so they are visible)(for IHC, retrieval will fade the
stain anyways)
2. After the stain, run slide
Our cassette printer finally gave up on us, and we are looking at available
options.
Does anyone have experience with the Brady cassette labeling system? We would
really appreciate ANY feedback about this product. We are also open to
suggestions. We are a veterinary lab with budget
Dear sirs.
I don't know whether I send this question to you.
For several weeks, I'm setting oil red O stain in fattty change liver (mouse).
Could you review my protocol?
1. Murine tissue,livers, is fixed with 10% NBF(10% neutral-buffered formalin).
2. Make frozen block
3. Blocks were cut 5um
We do this stain on frozen sections in our lab with no formalin fixation. We
stain in oil red o from American Mastertech for 30 minutes, rinse in water,
counterstain in hematoxylin, coverslip with aqueous mounting media.
Lisa
-Original Message-
From:
Hello,
I'm posting this request for a colleague, who is in need of normal monkey
placenta (preferably Rhesus). If anyone has some to spare, please let me know
and I'll forward your response.
Thanks,
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
Does anyone have an inexpensive vendor for activated carbon for the processor?
Thanks,
Travis
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We use pre-fixed tissue for oil red o. We rinse the section briefly to
remove the formalin from the outside of the tissue. We prepare our tissue
in OCT or the equivalent. We cut the frozen sections and mount them on
charged slides. We let dry the dry a minimum of 10 minute, rinse them in
Which processor?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Travis
Troyer
Sent: Thursday, April 14, 2011 12:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Activated Carbon
Does anyone
Go to any pet store and by activated carbon for a fish tank filter.
Empty out the old filter and replace the carbon. It's messy, but 90%
cheaper than buying med supply carbon.
Sincerely,
Jay A. Lundgren, M.S., HTL (ASCP)
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Histonet
Hello everyone, I have a question about hematoxylin hue on the HE stained
slides. We were not having this problem in the past but all of a sudden have
encountered it. We have changed out the entire solutions on the stainer and
checked protocols. Not sure why we are having this problem. Could
Hello,
Has anyone studied where un-nucleated squamous epithelial cells originate from?
I'm leaning towards thumbing blocks with an ungloved hand but don't know
for sure. Other possibilities I've come up with are dandruff from the head or
uncovered arms. I hate to see our histologist work in
Check the time in the acid alcohol after nuclear staining.
René J.
--- On Thu, 4/14/11, Michele Carr michelecar...@yahoo.com wrote:
From: Michele Carr michelecar...@yahoo.com
Subject: [Histonet] hematoxlin hue on slides
To: histonet@lists.utsouthwestern.edu
Date: Thursday, April 14, 2011, 1:36
Any extra-dried skin can shed cells. The best option is having your HTs using
some skin lotion (like Keri lotion) to prevent shedding of cells.
René J.
--- On Thu, 4/14/11, Charles, Roger rchar...@state.pa.us wrote:
From: Charles, Roger rchar...@state.pa.us
Subject: [Histonet] un-nucleated
If lotion gets in the waterbath, the tissue will not stick to the slides.
Shed skin cells can fall from any exposed skin, not just the hands. Like from
the face of those that do not wear make-up or lotion.
Paula
From: Rene J Buesa rjbu...@yahoo.com
To:
Can anyone give me any information Re: Thermo Scientific
Printmate?
I would like to get general information about this
equipment including maintenance experience, dependability, efficiency,
and any other experiences you have had either liked or dislike about
Robin,
Here is my take on the product from what I have observed. We are a high
volume teaching hospital.
ProsCons
SizeCost
Clean BarcodeService/Support
Low MaintenanceCost
Can be Networked
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