We are looking into new grossing stations. Can anybody give me their opinions
on which to choose, experiences, and what to avoid?
1. Mopec's Grossing Stations
2. ThermoFisher Gross Lab Senior
3. Accu-Edge Workstations by Sakura
James Vickroy BS, HT(ASCP)
Surgical and
I'm hoping someone can help me out. Our university has acquired a Microm HM
335 e Microtome, but we have no instruction manual. So far, the internet has
yielded no assistance. Does anyone know where I can find/get one, preferably
free?
Lindsey M. Minton
Thanks Histonet and all of you that responded! I almost immediately got a
response from a Thermo Fisher tech (Marganne you rock!) with the PDF.
Lindsey M. Minton
Jacksonville State University
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Hi Bret,
If you grab the first section as it comes off the blade with a pair of
forceps, then your ribbon never needs to touch the blade holder at all.
Just don't use your fingers!
Good luck,
-Eric
On Thu, Apr 21, 2011 at 12:07 PM, Clough, Bret
clo...@medicine.tamhsc.eduwrote:
While
Does anyone know what the positive control for MET is?
Thanks
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
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Histonet,
I like this site, but I am receiving way too much emails for one day. I have
requested to be removed from the mailing list several times to only receive
more emails. Am I inscribing incorrectly? Please help me dissolve this
relationship.
Thanks,
Kimberly Brown
Histology Lab
Go to the same website where your inscribed yourself, follow the instructions
and unscribe yourself. You are the ONLY one that can do that. Nobody can (or
will) do it for you.
René J.
--- On Tue, 4/26/11, Brown, Kimberly ksbr...@lmhosp.org wrote:
From: Brown, Kimberly ksbr...@lmhosp.org
Merci pour votre message.
Je suis actuellement absent du laboratoire.
Je repondrai a votre message a mon retour a partir du 3 mai 2011.
Thank you for your email.
I am currently out of the lab.
I will reply to your message after the 3rd of may 2011.
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Hi
I was given blocks today of a canine tooth that had been decaled for 8 days.
We processed it on our regular overnight program. It was too hard to cut and
popped out of the paraffin. The biopsy trim sheet did not indicate it was a
tooth so I put the pieces in ammonium hydroxide for 10
I would decal with TBD-2 for about two weeks. TBD-2 is a formic acid
decal solution. I would shake the specimen 24 hrs a day. Vacuum and
release the specimen several times a day. Change the decal every couple
of days. Do not remove the paraffin it helps to protect from over
decalcifying.
Sarah,
I worked this antibody up using normal colon. Uterine cervix, lung, and
gallbladder (all normal) should also stain nicely.
Michelle Mathews, HT(ASCP)
Research Specialist
TPL
Does anyone know what the positive control for MET is?
Thanks
Sarah Goebel, BA, HT(ASCP)
Being a MIXTURE of hydrocarbons, it will crack at different temperatures
(separately). You would have to reconstitute it using the same proportions
(usually proprietary).
René J.
--- On Tue, 4/26/11, Dawn Herron dwenze...@gmail.com wrote:
From: Dawn Herron dwenze...@gmail.com
Subject:
I work in a dermpath lab. One day last week we had a few slides that had
vacuoles or holes next to the nucleus. We had over 600 hundred cases, and it
only happened to a few of them. There seems to be nothing in common with them.
The biopsies came from different Dr's offices, different companies
This vacuolation was caused by you. The slides were not properly drained
(elimination of ALL the water from the back of the section) before they were
put on the oven to dry before staining.
Under separate cover I am sending you an article on the subject.
René J.
--- On Tue, 4/26/11, cindy dewar
Dr. Dean,
Histotechs are familiar with brain degeneration Silver Stains on FFPE cut
sections(slides). Common silver stains include Bielchowsky's method for
neuritic plaques and neurofibrillary tangles (most requested by my pathologists
for degenerative brain disorders), Bodian's method
I would think that the reason for the recut is relevant to the discussion. We
have a multi surgeon Mohs Surgery clinic and I've tracked recut requests by
surgeon and the data illustrates what I already knew. We have one surgeon who
prefers to go deeper into virtually every block. In this
I would appreciate hearing from anyone who has discovered an S100 primary
antibody that is confirmed to work on frozen sections using a conventional IHC
stain protocol. This will be utilized in a busy Mohs Surgery laboratory where
staining time must be kept as brief as possible.
The
Hey all,
We are looking for a PRN HT(ASCP) to help us out for holidays and the
unexpected suprises. Go to LUHCARES.ORG to get al the info and to apply.
thanks
Matt Lunetta
HT(ASCP)
Longmont United Hospital
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Contact Thermo at 800.522.7270.They own Microm.
Jim
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lindsey Minton
Sent: Tuesday, April 26, 2011 9:27 AM
To: histonet@lists.utsouthwestern.edu
Subject:
This is important.
Ensure that your fixation time is as long as possible.
Short processing will give poor results if fixation is shortened
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
An interesting article by Chun Gao, Ai-Ying Wang, and Ya-Jing Han (Appl
Immunohistochem Mol Morphol 2008;16:393-399) concluded that blockage of
endogenous peroxidase with H2O2 in immunostaining could be omitted and replaced
by microwave oven heating (using 0.01M citric acid buffer (pH 6.0)).
Bret - You can also check the back side of your blade. We wipe the front of
the blade often, but forget about the back. If paraffin builds up on the
blade the sections will stick.
Cheryl
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