Hi folks - has anyone out there found a Cd79α clone and supplier which will
cover multiple species reliably?
I can find clones which will stain cats and dogs (and humans), and clones which
will cover horses, pigs, cows and primates (and humans, but not opossums for
some reason??) but i'm yet to
I will be out of the office starting 05/12/2011 and will not return until
05/16/2011.
Note: For Cytology issues, please call Molly (day) at 8-421-5487 or Eric
(eve) at 8-421-5405, For Histology issues, please call the general
histology lab 8-421- 5408, Mario 8-421-4961 (day), Kiran 8-421
The best tip I ever received was from a gentleman in Australia who suggested
adding a very small - so small it would appear to be useless - amount of Tween
20 to the floatation water bath. The entrapment of water is eliminated - no
more blebs of water at the base of the sections. Works like ma
Another individual called me and commented further, which clarified your
statement. It is actually poor lab practice to combine the drying/baking.
These steps should really be done separately so as to not "trap" the water
under the tissue. I have noticed bubbles of water on the bottom of my s
I did Thanks!
I remain yours truely,
Candice Camille
From: Richard Cartun
To: Candice Smoots
Sent: Thu, May 12, 2011 1:56:19 PM
Subject: Re: [Histonet] H and E tumor
Did you get what you needed?
Richard
Richard W. Cartun, MS, PhD
Director, Histolog
So there really isn't a point in using an oven to dry the slides? I currently
dry my slides for an hour at 55-60 degrees C.
Thanks,
Nacaela Johnson, B.S. HTL (ASCP)CM
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office: 913-234-0576
Fax: 913-433-7639
E
Nacaela:
Heat by itself is not the cause, but the heat applied to sections that have not
been properly drained and still have water left between them and the slide, is.
You have to drain the sections properly before heating them.
Using formalin is not the cause.
René J.
From: "Johnson, Nacaela"
thanks linda I think that sounds good, I am going to do that.
anita
> Subject: RE: [Histonet] ventana validation
> Date: Thu, 12 May 2011 13:44:52 -0500
> From: lseb...@uwhealth.org
> To: azdud...@hotmail.com; histonet@lists.utsouthwestern.edu
>
> We run a control with the new kit and compa
We did not plan well so getting the EBER up and running was priority
one. I just entered the 4 ml. Working up the protocol --what was best in
our hands was protease 2- 4 mins, probe, then 2 -8 min stringency washes
at 37 degrees. Perhaps now I can work on diluting it. If you get some
info on th
We run a control with the new kit and compare it with a control that has
been run with the current kit (kept in a detection kit QC file). We
keep it as simple as possible with the least amount of cost we can
manage.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
D
ProPath, a progressive, CAP accredited, high volume, pathology practice,
located in Dallas, Texas, is seeking a Histotechnologist. The hours for this
position are Monday through Friday, 2:00 pm to 10:30 pm. In this position
you will be responsible for embedding tissue specimens, microtomy of
para
http://decapoda.nhm.org/pdfs/1505/1505.pdf
Here you can find an assay about formalin fixation. On page 851 there is a
description of nuclear and cytoplasmic bubbling found few minutes after
addition of formaldehyd-solution to cultured cells.
My interpretation is, that too short fixation makes t
would someone send me a copy of their validation that they do for the dab or
red ultra view kits? I
am not sure how to do this on the machine, do you do a control one one kit and
the same on the new?
just not sure what to do. thanks so much in advance
anita dudley
providence hosp
mobile al
Has anyone one had problems with nuclear bubbling? I have read about
two different causes. (1) Formalin itself is known to cause the issue
and (2) heating the tissue at a high temp (70 degrees C or above) while
drying. The latter is definitely not happening, but I do use formalin.
I am in the pr
I never heard of any such recommendations. DMSO doesn't react with
plastics or metals, and those are the only surfaces the embedding medium
comes into contact with, so I wouldn't expect any problems. In any
case, from a personal perspective, I used Paraplast Plus, which contains
DMSO, in my proces
Kansas State University in Manhattan, KS, is looking for a lab
supervisor/histotechnician/immunohistochemistry candidate to work in a
veterinary diagnostic histology/immunohistochemistry laboratory in the College
of Veterinary Medicine. Manhattan, KS has been recognized as one of the top
place
Dear Histonet members:
I am interesting in see the filopodia of the endothelial cells in the
corpus luteum of mouse. I have stained 25um cryostat sections of ovary
with an antibody agaisnt CD31 (PECAM) and alexa fluor and see it in the
confocal microscope, but I haven't succes. someone can tel
DMSO is always used in very small amounts and I am not aware of any
restrictions about its use with any type of tissue processor.
René J.
From: Debra Siena
To: "histonet@lists.utsouthwestern.edu"
Sent: Wednesday, May 11, 2011 6:30 PM
Subject: [Histonet] DMSO in Tissue processor
Hi Everyone,
I
Hi guys,
I was wondering if anyone had a picture of a tumor stained with H and E.
Please reply with it if you do. It does not matter the tissue. Thanks
I remain yours truely,
Candice Camille
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